CytoTox-ONE™ Homogeneous Membrane Integrity Assay

The CytoTox-ONE™ Homogeneous Membrane Integrity Assay is a fluorometric method for estimating the number of nonviable cells present in multiwell plates. The assay rapidly measures the release of lactate dehydrogenase (LDH) from cells with a damaged membrane. LDH released into the culture medium is measured with a 10-minute coupled enzymatic assay that results in the conversion of resazurin into a fluorescent resorufin product. The amount of fluorescence produced is proportional to the number of lysed cells using a 96- or 384-well format. The CytoTox-ONE™ Reagent does not damage normal healthy cells; therefore the reactions set up to measure released LDH can be performed directly in a homogeneous format in assay wells containing a mixed population of viable and damaged cells.

The CytoTox-ONE™ Homogeneous Membrane Integrity Assay, HTP (Cat.# G7892), offers convenient, alternative packaging for processing multiple plates. Each bottle of reagent supplied with the system is sufficient to perform 500 assays in a 96-well format or 2,000 assays in a 384-well format when the recommended volumes are used.

Save time by setting up the assay in a cell culture plate; this eliminates the sample transfer step common in many LDH assays. Plates are then incubated for just 10 minutes before reading data, compared to 30 minutes or more with classic LDH assays.

Multiplexing capability allows you to perform multiple assays on one sample with other homogeneous cell-based assays from Promega.

Adapt the assay protocol to your needs; completed assays can be read over several hours after the provided stop solution has been added while still maintaining good signal.