Coelenterazines

Luciferases from Renilla, Aequorea and other marine organisms are commonly used as indicators or reporters for studying cellular phenomena in expression assays in eukaryotic cells. Renilla luciferase is often used as a reporter of transcription regulation, whereas apoaequorin is often used as a calcium indicator. Other uses of coelenterazines include chemiluminescent detection of Reactive Oxygen Species (ROS) in cells or tissues. We offer the following coelenterazine analogs:

Coelenterazine (native) is the luminescent substrate for Renilla luciferase and apoaequorin. Formula: C₂₆H₂₁N₃O₃. Formula Weight: 423.5. Form: Film.

Coelenterazine-h imparts a luminescent intensity with its aequorin complex that is reported to be 10–20 times higher than that of native coelenterazine, making this derivative a useful tool for measuring small changes in Ca²⁺ concentrations. Formula: C₂₆H₂₁N₃O₂. Formula Weight: 407.5. Form: Film.

Learn more about custom options for this product at: www.promega.com/custom/

References

1. Inouye, S. et al. (1997) Biochem. and Biophys. Res. Comm. 233, 349–53.
2. Blanpain, C. et al. (2001) J. Biol. Chem. 276, 23795–804.
3. Shimomura, O. et al. (1989) Biochem. J. 261, 913–20.