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Technical Resources

Protocols

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Amplification

Routine PCR
Real-Time qPCR and qRT-PCR
RT-PCR
cDNA Synthesis and Primers
Nucleotides
PCR Cloning
PCR Purification

ROUTINE PCR PDF (size)
9PIM500 GoTaq® Hot Start Polymerase 63kb
TB254 GoTaq® PCR Core Systems 252kb
9PIM750 PCR Master Mix 59kb
9PIC114 PCR Nucleotide Mix 74kb
TB247 TaqBead™ Hot Start Polymerase 215kb

REAL-TIME qPCR and qRT-PCR PDF (size)
TM262 Plexor™ qPCR System 323kb
TM263 Plexor™ One-Step qRT-PCR System 281kb
TM264 Plexor™ Two-Step qRT-PCR System 274kb
TM265 Plexor™ Systems Instrument Setup and Data Analysis for the Applied Biosystems 7300 and 7500 Real-Time PCR Systems 5,592kb
TM266 Plexor™ Systems Instrument Setup and Data Analysis for the ABI PRISM® 7000 Sequence Detection System 4,142kb
TM267 Plexor™ Systems Instrument Setup and Data Analysis for the ABI PRISM® 7700 Sequence Detection System 3,648kb
TM268 Plexor™ Systems Instrument Setup and Data Analysis for the Applied Biosystems 7900HT Real-Time PCR System 4,187kb
TM269 Plexor® Systems Instrument Setup and Data Analysis for the Roche LightCycler® 1.0 and LightCycler® 1.5 Systems using LightCycler® Software Version 3.5 2,024kb
TM270 Plexor™ Systems Instrument Setup and Data Analysis for the Bio-Rad iCycler iQ™ Real-Time PCR Detection System 5,795kb
TM271 Plexor™ Systems Instrument Setup and Data Analysis for the DNA Engine Opticon® 2 Real-Time PCR Detection System 6,759kb
TM272 Plexor™ Systems Instrument Setup and Data Analysis for the SmartCycler® System 5,885kb
TM273 Plexor™ Systems Instrument Setup and Data Analysis for the Stratagene Mx3000P™ and Mx3005P™ Real-Time PCR Systems and Mx4000® Multiplex Quantitative PCR System 4,285kb
TM277 Plexor™ Systems Instrument Setup and Data Analysis for the Roche LightCycler® 2.0 System and LightCycler® 1.5 Systems using LightCycler® Software Version 4.0 2,312kb
TM290 Plexor™ Systems Instrument Setup and Data Analysis for the Roche LightCycler® 480 System 3,013kb
TM293 Plexor® HY System for the Applied Biosystems 7500 and 7500 FAST Real-Time PCR Systems 9,279kb
TM294 Plexor® HY System for the Stratagene Mx3000P® and Mx3005P™ Quantitative PCR Systems 9,548kb
TM296 Plexor® HY System for the Bio-Rad iQ™5 Real-Time PCR Detection System 10,879kb
TM299 Plexor® HY System for the Corbett Rotor-Gene 6000 Series Detection System 4,679kb
TM302 Plexor® HY System for the Roche LightCycler® 480 3,173kb
 
RT-PCR PDF (size)
9PIA170 AccessQuick™ RT-PCR System 46kb
TB220 Access RT-PCR System 260kb
TM236 ImProm-II™ Reverse Transcription System 388kb
TB099 Reverse Transcription System 147kb
9PIM210 Tth DNA Polymerase 60kb

cDNA SYNTHESIS AND PRIMERS PDF (size)
TM038 Universal RiboClone® cDNA Synthesis System 323kb

NUCLEOTIDES PDF (size)
9PIC114 PCR Nucleotide Mix 74kb

PCR CLONING PDF (size)
TM042 pGEM®-T and pGEM®-T Easy Vector Systems 292kb
TM044 pTARGET™  Mammalian Expression Vector System 480kb

PCR PURIFICATION PDF (size)
TB118 Wizard® PCR Preps DNA Purification System 163kb
TB311 Wizard® SV 96 PCR Clean-Up System 196kb
TB308 Wizard® SV Gel and PCR Clean-Up System 191kb