Notes: The SV Total RNA Isolation System was used to isolate total RNA from gingivostomatitis tissue. The RNA was eluted from the basket with 35µl of water, which was passed through the basket three times. Eleven microliters of the eluted material was used for two-step RT-PCR. One microliter of each eluted sample was analyzed for genomic DNA contamination by PCR, and any samples that did amplify were put through the SV RNA System again. As reported by the authors, 'Genomic DNA contamination was undetectable in the vast majority of sample eluates after a single RNA extraction procedure. The remaining samples were subsequently shown to be free from detectable genomic DNA after RNA reextraction.' (1064)

Notes: The ReadyAmp™ Genomic DNA Purification System was used to isolate genomic DNA from 200µl of whole blood. The PCR-quality DNA was used for nested PCR amplification. (0527)

Notes: The factors BDNF and NT-3 were used to maintain cultures. RNasin^® Ribonuclease Inhibitor and Taq DNA Polymerase were used for RT-PCR of rat total RNA derived from arcuate and periventricular cultures from newborn rats as well as intermediate lobe cells from adult rats. (0745)

Notes: Total RNA was extracted from HEL-5J20 and used to generate a full length cDNA (576bp) of the calcium- and intergrin-binding protein using the Access RT-PCR System. The subsequent product was purified with the Wizard^® PCR Preps System, digested with restriction enzymes and cloned into a GST-fusion expression vector. (0214)

Notes: The Apoptosis Detection System, Fluorescein was used to identify apoptotic cells in C6 glioma cultures in response to various reagents. TUNEL Update: The Apoptosis Detection System, Fluorescein has been renamed to DeadEnd™ Fluorimetric TUNEL System. (0504)

Notes: Authors use the PolyATtract^® 1000 System to isolate mRNA from rat myometrium and use this in quantitative RT-PCR experiments. (1220)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from human skin biopsies. Two hundred nanograms of the isolated RNA was used for RT-PCR amplification with the Access RT-PCR System. For added sensitivity, nested PCR was performed as well. (0108)

Notes: The fmol^® DNA Cycle Sequencing System was used to sequence the PCR-amplified G3PDH alleles from a number of haplotypes of cassava. (0604)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate total DNA from virus-infected primary monkey fibroblasts. The isolated DNA was used for PCR. The standard protocol was modified somewhat in that the cells were lysed in 300µl of Nuclei Lysis Solution containing proteinase K overnight at 37°C. The DNA was resuspended at the equivalent of 500, 1000 and 2000 cells per microliter. (0729)

Notes: Subunits of the cDNA for methionine adenosyltransferase were amplified from a plasmid using Taq DNA Polymerase and directly cloned into the pTARGET™ Mammalian Expression T-Vector. The sequence of the cloned inserts were confirmed with the fmol^® DNA Cycle Sequencing System. The constructs were transiently transfected into COS-1 cells with the TransFast™ Reagent at a 1:1 ratio. Excellent detail is provided for the transfection. (1094)

Notes: The PolyATtract® System 1000 was used to isolate poly(A)+ RNA directly from 5637 human bladder carcinoma cells. The isolated RNA was used for RT-PCR. (1272)

Notes: RNA probes were generated with 35S-UTP using the Riboprobe® in vitro Transcription System. The labeled probes were used for in situ hybridization of 18µm sections of the hippocampus. (1298)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate genomic DNA from Campylobacter sp. Prior to isolation, the bacteria were washed in TE and resuspended in 1ml of TE at ~1 x 10⁹ cells/ml. The isolated DNA was used for AFLP analysis. (1203)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from RAW 264.7 macrophages treated for various times with endotoxin. The isolated RNA was used for RT-PCR analysis of High Mobility Group-1 gene expression as well as β-actin. RT-PCR was performed with the Access RT-PCR System. (0198)

Notes: Total RNA was isolated from canine left ventricle muscle with the RNAgents^® Total RNA Isolation System. The RNA was used for RT-PCR. T4 Polynucleotide Kinase was used to 5´-end label an oligo to generate a probe for cDNA library screening. (0912)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate total DNA from virus-immortalized T cells. The isolated DNA was used for PCR. (0483)

Notes: The RNAgents^® Total RNA Isolation System was used to isolated total RNA from cultured human gingival fibroblasts. The isolated RNA was used for real-time quantitative PCR. (0648)

Notes: Genomic DNA was isolated from mouse brain with the Wizard^® Genomic DNA Purification Kit. The isolated DNA was used for PCR. The RNasin^® Ribonuclease Inhibitor was used to protect RNA in RT-PCR. (0372)

Notes: In this paper, DNA visualized on a sequencing gel is stained with the SILVER SEQUENCE™ DNA Sequencing Systems, and PCR products are cloned into the pGEM^®-T Vector. (0187)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate total RNA from immortalized human marrow stromal cell lines, HS-5 and HS-27a, that were incubated with 2 ng/mL IL-1β or 5% conditioned media from HS-5 cell cultures. Poly (A)+ RNA was then isolated from total RNA with the PolyATtract^® mRNA Isolation System. The Poly (A)+ RNA was used in RT-PCR  to create amplimers containing T7 promoters. The PCR products were used as templates in in vitro transcription reactions to generate biotin-labeled probes for microarrays. (2760)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate viral DNA and genomic DNA from Vero cells infected with HSV-1. The isolated DNA was used for PCR. (1393)

Notes: The p53 cDNAs expressed from thymic lymphoma cells were amplified with the Access RT-PCR System and the resulting products were cloned with the pGEM^®-T Vector System. No details of the reaction or whether or not total RNA was used were provided. (0812)

Notes: Synthesis of the mod-D1 cDNA and PCR amplification was performed using the Access RT-PCR System. (0744)

Notes: The complete cDNA for the CD1.1 gene was amplified and subcloned into the pTARGET™ Mammalian Expression Vector. The 1020bp, 339 amino acid protein was stably expressed in CHO cells following selection with G-418 sulfate. Expression was confirmed by Northern blot and FACS analysis with an mAb to the CD1.1 protein. The pGEM®-T Vector System was used for routine cloning of both PCR and RT-PCR products. The Prime-a-gene® Labeling System was used create probes for cosmid library screening. (1303)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate total RNA from Sertoli cells and germ cells. The isolated RNA was used for RT-PCR-based differential display. (0288)