Karlsson, M., Contreras, J.A., Hellman, U., Tornqvist, H., Holm, C.
Notes: Tryptic peptides, produced with Sequencing Grade Modified Trypsin, of the purified lipase were used to generate primers for RT-PCR. The largest amplimer was purified and used to screen a lambda gt11 library. The isolated 303 amino acid clone and site-specific mutants were put into the pCI-neo Mammalian Expression Vector and expressed in COS cells. The transiently expressed proteins were assayed for esterase and lipase activity. (0960)