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Lu, H.G., Zhong, L., Chang, K.P. and Docampo, R.
Notes: Poly A+ RNA was isolated from the protozoan Leishmania mexicanna amazonesis total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM-T Vector. (1678)
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pGEM®-T Vector System I
pGEM®-T Vector System II
PolyATtract® mRNA Isolation System II with Magnetic Stand
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Zhou, Y.D., Barnard, M., Tian, H., Li, X., Ring, H.Z., Francke, U., Shelton, J., Richardson, J., Russell, D.W. and McKnight, S.L.
Notes: PCR products of 372bp and 426bp were subcloned into the pGEM®-T Vector and used to produce RNA probes for Northern blotting and in situ hybridization. (1552)
Imaizumi, K., Tsuda, M., Imai, Y., Wanaka, A., Takagi, T., Tohyama, M.
Notes: Superior cervical ganglia were maintained in culture with or without the 2.5S NGF. RNA was isolated from these cultures and used for differential display with RT-PCR. The RT-PCR products were cloned with the pGEM®-T Vector System. (0998)
Stover, P., Chen, L., Suh, J., Stover, D., Keyomarsi, K., Shane, B.
Notes: Tth DNA Polymerase was used to perform RT-PCR with a two-buffer protocol. (1936)
Tth DNA Polymerase
Bai, G. and Kusiak, J.W.
Notes: Reporter vectors (produced with the pGL2 Basic Vector) were transiently transfected into PC12 cells and promoter activity was measured in response to 2.5S NGF. Promoter constructs were generated by PCR, subcloned into the pGEM®-T Vector and sequenced. The pGEM®-luc Vector was used to generated an antisense luc RNA probe used in RNase protection assays (using RNase ONE™ Ribonuclease) to follow the transcription of luciferase DNA following NGF treatment. The early growth reaction transcription factor and variants were expressed in the TNT® SP6 Coupled Reticulocyte Lysate System and used to perform gel shifts with promoter elements. The SP1 Consensus Oligonucleotide was used to compete transcription factor binding to the promoter elements. (1493)
RNase ONE™ Ribonuclease
SP1 Consensus Oligonucleotide
TnT® SP6 Coupled Reticulocyte Lysate System
TnT® SP6 Coupled Reticulocyte Lysate System, Trial Size
Scarisbrick, I.A., Towner, M.D., Isackson, P.J.
Notes: PolyA+ RNA was isolated from rat total RNA using the PolyATtract® mRNA Isolation System and used for Northern analysis. The pGEM®-T Vector System was used to clone products from RT-PCR. (0447)
PolyATtract® mRNA Isolation System I (Refill for Z5200)
PolyATtract® mRNA Isolation System III with Magnetic Stand
PolyATtract® mRNA Isolation System IV (Refill for Z5300)
Kester, H.A., van der Leede, B.M., van der Saag, P.T. and van der Burg, B.
Notes: Poly(A+) RNA was isolated from total RNA extracted from T47D mammary carcinoma cell line using the PolyATtract® mRNA Isolation System. The RNA was used for northern blots. RQ1 was used to digest genomic DNA away from total RNA. The pGEM®-T Vector was used to subclone PCR products generated by differential display. (1673)
RQ1 RNase-Free DNase
Bahouth, S.W., Cui, X., Beauchamp, M.J., Shimomura, H., George, S.T. and Park, E.A.
Notes: Reporter studies were performed in primary rat ventricular myocytes, HepG2 cells and SK-N-MC cells using the pGL3 Basic Vector. All luciferase values were normalized to β-galactosidase activity provided by the pSV-β-Galactosidase Control Vector. PCR-generated deletion mutants of promoter constructs were subcloned into the pGEM®-T Vector and sequenced. (1491)
Luciferase Assay System
Luciferase Assay System with Reporter Lysis Buffer
pSV-β-Galactosidase Control Vector
Schäfer, M., Schütz, B., Weihe, E. and Eiden, L.
Notes: The pGEM®-T Vector System was used to clone a 270bp RT-PCR product from rat spinal cord mRNA.. (2007)
Ninkina, N. , Grashchuck, M. , Buchman, V. L. , Davies, A. M.
Notes: The PolyATtract® System 1000 was used to isolate polyA+ RNA directly from newborn mouse brains. The RNA was used in RT-PCR for detection of specific TrkB variants. Taq DNA Polymerase and the pGEM®-T Vector System were used to produce and subclone novel TrkB variants that differ in the extracellular domain. The CellTiter 96® Non-Radioactive Cell Proliferation Assay was performed on NIH 3T3 cells transfected with the TrkB receptors and challenged with BDNF, NT3 and NT4/5. (0615)
CellTiter 96® Non-Radioactive Cell Proliferation Assay
PolyATtract® System 1000 with Magnetic Stand
PolyATtract® System 1000 without Magnetic Stand
Laville, M., Auboeuf, D., Khalfallah, Y., Vega, N., Riou, J.P. and Vidal H.
Notes: Promega's Tth DNA Polymerase, pGEM®-T Vector System and Riboprobe® System were used in this study. (2002)
Riboprobe® Combination System-SP6/T7 RNA Polymerase
Riboprobe® Combination System-T3/T7 RNA Polymerase
Croteau, W., Davey, J.C., Galton, V.A. and St. Germain, D.L.
Notes: Promega's Access RT-PCR System was used in this study. (1997)
Access RT-PCR System
Zardoya, R. , Abouheif, E. , Meyer, A.
Notes: PCR products were cloned into the pGEM®-T Vector System. (0082)
Forleo, P., Couchie, D., Chabas, S. and Nunez, J.
Notes: The system was used to clone the amplimers generated by RT-PCR. Sequencing of the clones was performed in the vector. (1569)
Yang, Y. and Klessig, D.F.
Notes: The pGEM®-T Vector System was used to clone PCR products amplified from a tobacco cDNA library. (1968)
Firth, J. D. , Ebert, B. L. , Ratcliffe, P. J.
Notes: The Altered Sites® II in vitro Mutagenesis System was used to produce 14 consecutive 4bp deletions in a putative response element. (1157)
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