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J. Biol. Chem. 272, 9464-9473. Intracellular Ca2+ pool content and signaling and expression of a calcium pump are linked to virulence in Leishmania mexicana amazonesis amastigotes. 1997

Lu, H.G., Zhong, L., Chang, K.P., Docampo, R.

Notes: PolyATtract® mRNA Isolation System was used to isolate Poly A+ RNA from the protozoan Leishmania mexicanna amazonesis total RNA. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM®-T Vector. (0748)

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Proc. Natl. Acad. Sci. USA 94, 713-718. Molecular characterization of two mammalian bHLH-PAS domain proteins selectively expressed in the central nervous system. 1997

Zhou, Y.D., Barnard, M., Tian, H., Li, X., Ring, H.Z., Francke, U., Shelton, J., Richardson, J., Russell, D.W. and McKnight, S.L.

Notes: PCR products of 372bp and 426bp were subcloned into the pGEM®-T Vector and used to produce RNA probes for Northern blotting and in situ hybridization. (1552)

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J. Biol. Chem. 272, 18842-18848. Molecular cloning of a novel polypeptide, DP5, induced during programmed neuronal death. 1997

Imaizumi, K., Tsuda, M., Imai, Y., Wanaka, A., Takagi, T., Tohyama, M.

Notes: Superior cervical ganglia were maintained in culture with or without the 2.5S NGF. RNA was isolated from these cultures and used for differential display with RT-PCR. The RT-PCR products were cloned with the pGEM®-T Vector System. (0998)

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J. Biochem. (Tokyo) 272, 1842-1848. Molecular Cloning, Characterization, and Regulation of the Human Mitochondrial Serine Hydroxymethyltransferase Gene. 1997

Stover, P., Chen, L., Suh, J., Stover, D., Keyomarsi, K., Shane, B.

Notes: Tth DNA Polymerase was used to perform RT-PCR with a two-buffer protocol. (1936)

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J. Biol. Chem. 272, 5936-5942. Nerve growth factor up-regulates the N-methyl-D-aspartate receptor subunit 1 promoter in PC12 cells. 1997

Bai, G. and Kusiak, J.W.

Notes: Reporter vectors (produced with the pGL2 Basic Vector) were transiently transfected into PC12 cells and promoter activity was measured in response to 2.5S NGF. Promoter constructs were generated by PCR, subcloned into the pGEM®-T Vector and sequenced. The pGEM®-luc Vector was used to generated an antisense luc RNA probe used in RNase protection assays (using RNase ONE™ Ribonuclease) to follow the transcription of luciferase DNA following NGF treatment. The early growth reaction transcription factor and variants were expressed in the TNT® SP6 Coupled Reticulocyte Lysate System and used to perform gel shifts with promoter elements. The SP1 Consensus Oligonucleotide was used to compete transcription factor binding to the promoter elements. (1493)

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J. Neurosci. 17, 8156-8168. Nervous system-specific expression of a novel serine protease: Regulation in the adult rat spinal cord by excitotoxic injury 1997

Scarisbrick, I.A., Towner, M.D., Isackson, P.J.

Notes: PolyA+ RNA was isolated from rat total RNA using the PolyATtract® mRNA Isolation System and used for Northern analysis. The pGEM®-T Vector System was used to clone products from RT-PCR. (0447)

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J. Biol. Chem. 272(26), 16637-16643. Novel progesterone target genes identified by an improved differential display technique suggest that progestin-induced growth inhibition of breast cancer cells coincides with enhancement of differentiation. 1997

Kester, H.A., van der Leede, B.M., van der Saag, P.T. and van der Burg, B.

Notes: Poly(A+) RNA was isolated from total RNA extracted from T47D mammary carcinoma cell line using the PolyATtract® mRNA Isolation System. The RNA was used for northern blots. RQ1 was used to digest genomic DNA away from total RNA. The pGEM®-T Vector was used to subclone PCR products generated by differential display. (1673)

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Mol. Pharmacol. 51, 620-629. Promoter analysis of the rat β1-adrenergic receptor gene identifies sequences involved in basal expression. 1997

Bahouth, S.W., Cui, X., Beauchamp, M.J., Shimomura, H., George, S.T. and Park, E.A.

Notes: Reporter studies were performed in primary rat ventricular myocytes, HepG2 cells and SK-N-MC cells using the pGL3 Basic Vector. All luciferase values were normalized to β-galactosidase activity provided by the pSV-β-Galactosidase Control Vector. PCR-generated deletion mutants of promoter constructs were subcloned into the pGEM®-T Vector and sequenced. (1491)

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Proc. Natl. Acad. Sci. USA 94, 4149-4154. Target-independent cholinergic differentiation in the rat sympathetic nervous system. 1997

Schäfer, M., Schütz, B., Weihe, E. and Eiden, L.

Notes: The pGEM®-T Vector System was used to clone a 270bp RT-PCR product from rat spinal cord mRNA.. (2007)

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J. Biol. Chem. 272, 13019-13025. TrkB variants with deletions in the leucine-rich motifs of the extracellular domain. 1997

Ninkina, N. , Grashchuck, M. , Buchman, V. L. , Davies, A. M.

Notes: The PolyATtract® System 1000 was used to isolate polyA+ RNA directly from newborn mouse brains. The RNA was used in RT-PCR for detection of specific TrkB variants. Taq DNA Polymerase and the pGEM®-T Vector System were used to produce and subclone novel TrkB variants that differ in the extracellular domain. The CellTiter 96® Non-Radioactive Cell Proliferation Assay was performed on NIH 3T3 cells transfected with the TrkB receptors and challenged with BDNF, NT3 and NT4/5. (0615)

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J. Clin. Invest. 98(1), 43-49. Acute regulation by insulin of phosphatidylinositol-3-kinase, Rad, Glut 4, and lipoprotein lipase mRNA levels in human muscle. 1996

Laville, M., Auboeuf, D., Khalfallah, Y., Vega, N., Riou, J.P. and Vidal H.

Notes: Promega's Tth DNA Polymerase, pGEM®-T Vector System and Riboprobe® System were used in this study. (2002)

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J. Clin. Invest. 98(2), 405-417. Cloning of the mammalian Type II Iodothyronine Deiodinase: A selenoprotein differentially expressed and regulated in human and rat brain and other tissues. 1996

Croteau, W., Davey, J.C., Galton, V.A. and St. Germain, D.L.

Notes: Promega's Access RT-PCR System was used in this study. (1997)

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Proc. Natl. Acad. Sci. USA 93, 13036-13041. Evolutionary analyses of hedgehog and Hoxd-10 genes in fish species closely related to the zebrafish. 1996

Zardoya, R. , Abouheif, E. , Meyer, A.

Notes: PCR products were cloned into the pGEM®-T Vector System. (0082)

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J. Mol. Neurosci. 7, 193-201. Four repeat high-mol-wt MAP2 forms in rat dorsal root ganglia. 1996

Forleo, P., Couchie, D., Chabas, S. and Nunez, J.

Notes: The system was used to clone the amplimers generated by RT-PCR. Sequencing of the clones was performed in the vector. (1569)

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Proc. Natl. Acad. Sci. USA 93, 14972-14977. Isolation and characterization of a tobacco mosaic virus-inducible myb oncogene homolog from tobacco. 1996

Yang, Y. and Klessig, D.F.

Notes: The pGEM®-T Vector System was used to clone PCR products amplified from a tobacco cDNA library. (1968)

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J. Biol. Chem. 270, 21021-21027. Hypoxic regulation of lactate dehydrogenase A. Interaction between hypoxia-inducible factor 1 and cAMP response elements. 1995

Firth, J. D. , Ebert, B. L. , Ratcliffe, P. J.

Notes: The Altered Sites® II in vitro Mutagenesis System was used to produce 14 consecutive 4bp deletions in a putative response element. (1157)

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