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Genetics 150, 1625-1637. Extraordinary ribosomal spacer length heterogeneity in a neotyphodium endophyte hybrid: implications for concerted evolution. 1998

Ganley, A. R. , Scott, B.

Notes: Serial deletions of a 4.1kb insert were made by using the Erase-a-Base® System for DNA sequencing. (1138)

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Genetics 149, 1081-1088. Gene silencing by DNA methylation and dual inheritance in Chinese hamster ovary cells. 1998

Paulin, R. P., Ho, T., Balzer, H. J., Holliday, R.

Notes: Bisulphite-treated DNA was purified using the Wizard® DNA Clean-Up System prior to PCR. The amplified DNA was then cleaned using a Wizard® PCR Preps DNA Purification System prior to cloning and the cloned DNAs were sequenced using the fmol® DNA Cycle Sequencing System. (0001)

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Proc. Natl. Acad. Sci. USA 95, 9654-9659. Human endplate acetylcholinesterase deficiency caused by mutations in the collagen-like tail subunit (ColQ) of the asymmetric enzymes. 1998

Ohno, K., Brengman, J., Tsujino, A., Engel, A.G.

Notes: The entire coding region of the T isoform of the catalytic subunit of the acetycholinesterase (ACHET)was amplified and cloned into the pTargeT™ Vector and sequenced. The ColQ cDNA was cloned into the pTargeT™ Vector as well and mutated with a Pfu DNA polymerase-based mutagenesis system. The ACHET and ColQ mutants were coexpressed in COS cells and interactions examined with sedimentation analysis through sucrose gradients. (0595)

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Proc. Natl. Acad. Sci. USA 95, 2761-2766. Insect juvenile hormone resistance gene homology with the bHLH-PAS family of transcriptional regulators. 1998

Ashok, M., Turner, C. and Wilson, T.G.

Notes: In this paper, a 538bp fragment was amplified and subcloned into the pGEM®-T Easy Vector. (1483)

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Genetics 150, 1125-1131. Mouse Brachyury the Second (T2) is a gene next to classical T and a candidate gene for tct. 1998

Rennebeck, G., Lader, E., Fujimoto, A., Lei, E.P., Artzt, K.

Notes: Streptavidin MagneSphere® Paramagnetic Particles (SA-PMPs) were used to capture biotinylated primer probe in a random access retrieval of genetic information by PCR (rargip) screening [ABE, K., (1992) Rapid isolation of desired sequences from lone linker PCR amplified cDNA mixtures: application to identification and recovery of expressed sequences in cloned genomic DNA. Mamm. Genome 2:252-259]. The pGEM®-T Vector System and PolyATract® mRNA Isolation System were also used. (0513)

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J. Neurosci. 18, 16-25. Regulation of Ca2+-dependent K+ channel expression in rat cerebellum during postnatal development. 1998

Muller, Y.L., Reitstetter, R., Yool, A.J.

Notes: Poly-A+ RNA was isolated from rat cerebellar total RNA with the PolyATtract® mRNA Isolation System and used for semi-quantitative RT-PCR. The targets of the RT-PCR were cloned with the pGEM®-T Vector System and sequenced to show they quantitated the correct targets. (0671)

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Biochemistry 37, 4420-4428.. Silent nucleotide substitution in the sterol 27-hydrosylase gene (CYP 27) leads to alternative pre-mRNA splicing by activating a crytic 5' splice site at the mutant codon in cerebrotendinous xanthomatosis patients. 1998

Chen, W., Kubota, S., Teramoto, T., Nishimura, Y., Yonemoto, K., Seyama, Y.

Notes: Genomic analysis noted a single base change between wild type and mutant. To see if this base change was enough for alternative mRNA splicing, minigenes were constructed and subcloned into the pTargeT™ Vector. The mutant and wild-type minigenes were transfected into COS-1 cells. Forty-eight hours post-transfection total RNA was isolated and analyzed by RT-PCR. Transfectants with the mutant sequence did produce a different product due to alternative splicing. (1331)

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J. Biol. Chem. 273, 25680-25685. The human chitotriosidase gene: Nature of inherited enzyme deficiency. 1998

Boot, R.G., Renkema, G.H., Verhoek, M., Strijland, A., Bliek, J., de Meulemeester, T.M.A.M.O., Mannens, M.M.A.M., Aerts, J.M.F.G.

Notes: The pGEM®-5Zf(+) Vector was used for subcloning of a PCR fragment. The fragment was used to make a probe for RNase protection assays. The pGEM®-7Zf(+) Vector was used for routine subcloning of restriction fragments from the chitotriosidase gene. (1427)

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Proc. Natl. Acad. Sci. USA 94, 6216-6221. A new member of the tumor necrosis factor/nerve growth factor receptor family inhibits T cell receptor–induced apoptosis. 1997

Nocentini, G., Giunchi, L., Ronchetti, S., Krausz, L.T., Bartoli, A., Moraca, R., Migliorati, G. and Riccardi, C.

Notes: The TNT® Coupled Transcription/Translation System was used to verify the 25.3kDa size of the cloned receptor. AMV reverse transcriptase was used for first strand cDNA synthesis prior to PCR. (1520)

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Blood 90(12), 5013-5502. A novel five-transmembrane hematopoietic stem cell antigen: Isolation, characterization, and molecular cloning. 1997

Miraglia, S., Godfrey, W., Yin, A.H., Atkins, K., Warnke, R., Holden, J.T., Bray, R.A., Waller, E.K. and Buck, D.W.

Notes: Promega's Access RT-PCR system, Taq DNA Polymerase and PolyATtract® mRNA Isolation System were used in this study. (1991)

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Proc. Natl. Acad. Sci. USA 94, 1069-1073. Assembly of an active enzyme by the linkage of two protein modules. 1997

Nixon, A. E. , Warren, M. S. , Benkovic, S. J.

Notes: Taq DNA Polymerase was used for PCR. The PCR products were purified with the Wizard® PCR Preps DNA Purification System and cloned with the aid of the pGEM®-T Vector System. (0624)

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Immunity 6, 119-129. cDNA cloning and primary structure analysis of C1qR(P), the human C1q/MBL/SPA receptor that mediates enhanced phagocytosis in vitro. 1997

Nepomuceno, R.R., Henschen Edman, A.H., Burgess, W.H., Tenner, A.J.

Notes: RT-PCR was performed with degenerate primers and the resulting 110bp product was subcloned with the pGEM®-T Vector System. The 110bp fragment was used to screen a cDNA library and four positive plaques were identified. The lambda clones were sequenced with the fmol® DNA Cycle Sequencing System. To sequence across the entire lambda clone nested deletions were made with the Erase-a-Base® System. (0650)

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J. Biol. Chem. 272, 27218-27223. cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases. 1997

Karlsson, M., Contreras, J.A., Hellman, U., Tornqvist, H., Holm, C.

Notes: Tryptic peptides, produced with Sequencing Grade Modified Trypsin, of the purified lipase were used to generate primers for RT-PCR. The largest amplimer was purified and used to screen a lambda gt11 library. The isolated 303 amino acid clone and site-specific mutants were put into the pCI-neo Mammalian Expression Vector and expressed in COS cells. The transiently expressed proteins were assayed for esterase and lipase activity. (0960)

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Proc. Natl. Acad. Sci. USA 94(7), 3206-3210. Cloning and characterization of the extreme 5'-terminal sequences of the RNA genomes of GB virus C/hepatitis G virus. 1997

Hsieh, S. Y., Yang, P. Y., Chen, H. C., and Liaw, Y. F.

Notes: The RNasin® Ribonuclease Inhibitor and the pGEM®-T Vector System were used in this study. The inhibitor was used to protect RNA during the T4 RNA ligase step for 5' RACE analysis. (1643)

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J. Biol. Chem. 272(9), 5464-5468. Cloning and localization of a glutathione S-transferase class I gene from Anopheles gambiae. 1997

Ranson, H., Cornel, A.J., Fournier, D., Vaughan, A., Collins, F.H. and Hemingway, J.

Notes: The PolyATtract® mRNA Isolation System was used to isolate poly A+ RNA from Anopheles gambiae (mosquito) total RNA. The isolated RNA was used for cDNA synthesis with the Universal Riboclone System. (1683)

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J. Neurosci. 17, 765-773. Consequences of nigrostriatal denervation of the functioning of the basal ganglia in human and nonhuman primates: An in situ hybridization study of cytochrome oxidase subunit I mRNA. 1997

Vila, M., Levy, R., Herrero, M.T., Ruberg, M., Faucheux, B., Obeso, J.A., Agid, Y. and Hirsch, E.C.

Notes: A portion of the cytochrome oxidase subunit I cDNA was amplified and cloned into the pGEM®-T Vector. RNA probes were produced from the pGEM®-T Vector for in situ hybridization studies. (1547)

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J. Neurosci. 17, 6094-6104.. Detection of functional nicotinic receptors blocked by alpha-bungarotoxin on PC12 cells and dependence of their expression on post-translational events. 1997

Blumenthal, E.M., Conroy, W.G., Romano, S.J., Kassner, P. D. and Berg, D. K.

Notes: NGF was used to induce PC12 cell differentiation 3-5 days prior to assay. PCR amplimers representing various subunits of the acetylcholine receptor were subcloned into the pGEM®-T Vector. The resulting clones were used to produce RNA probes for RNase protection assays. Also, a chimeric a5/7-HT3 receptor was generated by PCR, subcloned into the pGEM®-T Vector and sequenced. (1419)

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J. Biol. Chem. 272, 19538-19546. Disassembly of RanGTP-Karyopherin beta Complex, an Intermediate in Nuclear Protein Import. 1997

Floer, M. , Blobel, G. , Rexach, M.

Notes: Promega's Saccharomyces cerevisiae genomic DNA was used as the template for PCR amplification of several genes. (1161)

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Products

J. Neurosci. 17, 9145-9156. Estradiol Enhances Prostaglandin E2 Receptor Gene Expression in Luteinizing Hormone-Releasing Hormone (LHRH) Neurons and Facilitates the LHRH Response to PGE2 by Activating a Glia-to-Neuron Signaling Pathway 1997

Rage, F., Lee, B.J., Ma, Y. J., Ojeda, S. R.

Notes: Taq DNA Polymerase was used in RT-PCR reactions and the products examined by Southern hybridization. Promising bands were cut from the agarose gel and cloned with the pGEM®-T Vector System. (0529)

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Proc. Natl. Acad. Sci. USA 94, 4211-4216. Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6- desaturated fatty acids in transgenic tobacco. 1997

Sayanova, O., Smith, M.A., Lapinskas, P., Stobart, A.K., Dobson, G., Christie, W.W., Shewry, P.R., Napier, J.A.

Notes: The authors use the Reverse Transcription System, Wizard® PCR Preps DNA Purification Systems, Wizard® Plus Minipreps DNA Purification System and the pGEM®-T Vector System in their studies. (0445)

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J. Mol. Neurosci. 8, 13-18. Identification of a rat brain gene associated with aging by PCR differential display method. 1997

Wu, H.C. and Lee, E.H.Y.

Notes: The pGEM®-T Vector System was used to clone the products from PCR differential display. Sequencing of the insert was performed. (1550)

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J. Biol. Chem. 272, 9464-9473. Intracellular Ca2+ pool content and signaling and expression of a calcium pump are linked to virulence in Leishmania mexicana amazonesis amastigotes. 1997

Lu, H.G., Zhong, L., Chang, K.P., Docampo, R.

Notes: PolyATtract® mRNA Isolation System was used to isolate Poly A+ RNA from the protozoan Leishmania mexicanna amazonesis total RNA. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM®-T Vector. (0748)

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J. Biol. Chem. 272(14), 9464-9473. Intracellular Ca2+ pool content and signaling and expression of a calcium pump are linked to vrulence in Leishmania mexicana amazonesis amastigotes. 1997

Lu, H.G., Zhong, L., Chang, K.P. and Docampo, R.

Notes: Poly A+ RNA was isolated from the protozoan Leishmania mexicanna amazonesis total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM-T Vector. (1678)

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Proc. Natl. Acad. Sci. USA 94, 713-718. Molecular characterization of two mammalian bHLH-PAS domain proteins selectively expressed in the central nervous system. 1997

Zhou, Y.D., Barnard, M., Tian, H., Li, X., Ring, H.Z., Francke, U., Shelton, J., Richardson, J., Russell, D.W. and McKnight, S.L.

Notes: PCR products of 372bp and 426bp were subcloned into the pGEM®-T Vector and used to produce RNA probes for Northern blotting and in situ hybridization. (1552)

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J. Biol. Chem. 272, 18842-18848. Molecular cloning of a novel polypeptide, DP5, induced during programmed neuronal death. 1997

Imaizumi, K., Tsuda, M., Imai, Y., Wanaka, A., Takagi, T., Tohyama, M.

Notes: Superior cervical ganglia were maintained in culture with or without the 2.5S NGF. RNA was isolated from these cultures and used for differential display with RT-PCR. The RT-PCR products were cloned with the pGEM®-T Vector System. (0998)

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