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Biochemistry 37, 4420-4428.. Silent nucleotide substitution in the sterol 27-hydrosylase gene (CYP 27) leads to alternative pre-mRNA splicing by activating a crytic 5' splice site at the mutant codon in cerebrotendinous xanthomatosis patients. 1998

Chen, W., Kubota, S., Teramoto, T., Nishimura, Y., Yonemoto, K., Seyama, Y.

Notes: Genomic analysis noted a single base change between wild type and mutant. To see if this base change was enough for alternative mRNA splicing, minigenes were constructed and subcloned into the pTargeT™ Vector. The mutant and wild-type minigenes were transfected into COS-1 cells. Forty-eight hours post-transfection total RNA was isolated and analyzed by RT-PCR. Transfectants with the mutant sequence did produce a different product due to alternative splicing. (1331)

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Proc. Natl. Acad. Sci. USA 95, 15803-15808. Cloning of the cDNA encoding the urotensin II precursor in frog and human reveals intense expression of the urotensin II gene in motoneurons of the spinal cord. 1998

Coulouarn, Y. , Lihrmann, I. , Jegou, S. , Anouar, Y. , Tostivint, H. , Beauvillain, J. C. , Conlon, J. M. , Bern, H. A. , Vaudry, H.

Notes: In this paper, Tfl DNA polymerase was used for two-step RT-PCR. The amplified fragments were cloned into the pGEM®-T Vector. Promega's Terminal Deoxynucleotidyl Transferase (TdT) was used to end label specific oligos for screening a frog brain cDNA library. T7 and T3 RNA polymerases were used to make digoxigenin labeled riboprobes for in situ hybridization. (1281)

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Genetics 150, 1125-1131. Mouse Brachyury the Second (T2) is a gene next to classical T and a candidate gene for tct. 1998

Rennebeck, G., Lader, E., Fujimoto, A., Lei, E.P., Artzt, K.

Notes: Streptavidin MagneSphere® Paramagnetic Particles (SA-PMPs) were used to capture biotinylated primer probe in a random access retrieval of genetic information by PCR (rargip) screening [ABE, K., (1992) Rapid isolation of desired sequences from lone linker PCR amplified cDNA mixtures: application to identification and recovery of expressed sequences in cloned genomic DNA. Mamm. Genome 2:252-259]. The pGEM®-T Vector System and PolyATract® mRNA Isolation System were also used. (0513)

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J. Biol. Chem. 273, 25680-25685. The human chitotriosidase gene: Nature of inherited enzyme deficiency. 1998

Boot, R.G., Renkema, G.H., Verhoek, M., Strijland, A., Bliek, J., de Meulemeester, T.M.A.M.O., Mannens, M.M.A.M., Aerts, J.M.F.G.

Notes: The pGEM®-5Zf(+) Vector was used for subcloning of a PCR fragment. The fragment was used to make a probe for RNase protection assays. The pGEM®-7Zf(+) Vector was used for routine subcloning of restriction fragments from the chitotriosidase gene. (1427)

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Proc. Natl. Acad. Sci. USA 95, 229-234. A mitochondrial-like chaperonin 60 gene in Giardia lamblia: Evidence that diplomonads once harbored an endosymbiont related to the progenitor of mitochondria 1998

Roger, A., Svard, S., Tovar, J., Clark, C., Smith, M., Gillin, F. and Sogin, M.

Notes: pGEM®-T Easy Vector Systems (2068)

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Proc. Natl. Acad. Sci. USA 95, 2761-2766. Insect juvenile hormone resistance gene homology with the bHLH-PAS family of transcriptional regulators. 1998

Ashok, M., Turner, C. and Wilson, T.G.

Notes: In this paper, a 538bp fragment was amplified and subcloned into the pGEM®-T Easy Vector. (1483)

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Plant Cell 10, 297-308. a-Tubulin Missense Mutations Correlate with Antimicrotubule Drug Resistance in Eleusine indica. 1998

Yamamoto, E., Zeng, L. and Baird, W.

Notes: The pGEM® -T Easy Vector Systems, Wizard® PCR Preps DNA Purification System and Wizard® Plus Minipreps DNA Purification Systems were used in this study. (1959)

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J. Biol. Chem. 273, 27420-27429. Characterization of the muring fatty acid transport protein gene and its insulin response sequence. 1998

Hui, T.Y., Frohnert, B.I., Smith, A.J., Schaffer, J.E., Bernlohr, D.A.

Notes: Luciferase reporter constructs were cotransfected into 3T3-L1 preadipocytes with the pRL-CMV Vector at a 25:1 ratio and luciferase activities were determined with the Dual-Luciferase® Reporter Assay System. RT-PCR was accomplished with AMV Reverse Transcriptase and Taq DNA Polymerase. RNase Protection Assay were performed with the aid of the RNase ONE™ Ribonuclease. (0988)

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Biochemistry 37, 15050-15056. A novel Arg362Ser mutation in the sterol 27-hydroxylase gene (CYP27): Its effects on pre-mRNA splicing and enzyme activity. 1998

Chen, W., Kubota, S., Ujike, H., Ishihara, T., Seyama, Y.

Notes: A minigene was constructed with five exons and four introns from exons 5-9 of the CYP27 gene. The 2111bp product was generated with primers containing a start codon and a stop codon. The product was directly cloned into the pTARGET™ Mammalian expression vector and transfected into COS-1cells. RNA was isolated from these cells and RT-PCR was performed to amplify the spliced product. This assay could distinguish between normal splicing of the third intron and aberrant splicing of due to the Arg362Ser mutation. The proteins produced by the normal and mutant minigenes were also assessed by immunoblotting. (1333)

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Proc. Natl. Acad. Sci. USA 94, 1069-1073. Assembly of an active enzyme by the linkage of two protein modules. 1997

Nixon, A. E. , Warren, M. S. , Benkovic, S. J.

Notes: Taq DNA Polymerase was used for PCR. The PCR products were purified with the Wizard® PCR Preps DNA Purification System and cloned with the aid of the pGEM®-T Vector System. (0624)

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J. Biol. Chem. 272(14), 9464-9473. Intracellular Ca2+ pool content and signaling and expression of a calcium pump are linked to virulence in Leishmania mexicana amazonesis amastigotes. 1997

Lu, H.G., Zhong, L., Chang, K.P. and Docampo, R.

Notes: Poly A+ RNA was isolated from the protozoan Leishmania mexicanna amazonesis total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM-T Vector. (1678)

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J. Biol. Chem. 272, 19538-19546. Disassembly of RanGTP-Karyopherin beta Complex, an Intermediate in Nuclear Protein Import. 1997

Floer, M. , Blobel, G. , Rexach, M.

Notes: Promega's Saccharomyces cerevisiae genomic DNA was used as the template for PCR amplification of several genes. (1161)

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Proc. Natl. Acad. Sci. USA 94, 713-718. Molecular characterization of two mammalian bHLH-PAS domain proteins selectively expressed in the central nervous system. 1997

Zhou, Y.D., Barnard, M., Tian, H., Li, X., Ring, H.Z., Francke, U., Shelton, J., Richardson, J., Russell, D.W. and McKnight, S.L.

Notes: PCR products of 372bp and 426bp were subcloned into the pGEM®-T Vector and used to produce RNA probes for Northern blotting and in situ hybridization. (1552)

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J. Mol. Neurosci. 8, 13-18. Identification of a rat brain gene associated with aging by PCR differential display method. 1997

Wu, H.C. and Lee, E.H.Y.

Notes: The pGEM®-T Vector System was used to clone the products from PCR differential display. Sequencing of the insert was performed. (1550)

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J. Biol. Chem. 272(26), 16637-16643. Novel progesterone target genes identified by an improved differential display technique suggest that progestin-induced growth inhibition of breast cancer cells coincides with enhancement of differentiation. 1997

Kester, H.A., van der Leede, B.M., van der Saag, P.T. and van der Burg, B.

Notes: Poly(A+) RNA was isolated from total RNA extracted from T47D mammary carcinoma cell line using the PolyATtract® mRNA Isolation System. The RNA was used for northern blots. RQ1 was used to digest genomic DNA away from total RNA. The pGEM®-T Vector was used to subclone PCR products generated by differential display. (1673)

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J. Biol. Chem. 272(9), 5464-5468. Cloning and localization of a glutathione S-transferase class I gene from Anopheles gambiae. 1997

Ranson, H., Cornel, A.J., Fournier, D., Vaughan, A., Collins, F.H. and Hemingway, J.

Notes: The PolyATtract® mRNA Isolation System was used to isolate poly A+ RNA from Anopheles gambiae (mosquito) total RNA. The isolated RNA was used for cDNA synthesis with the Universal Riboclone System. (1683)

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Blood 90(12), 5013-5502. A novel five-transmembrane hematopoietic stem cell antigen: Isolation, characterization, and molecular cloning. 1997

Miraglia, S., Godfrey, W., Yin, A.H., Atkins, K., Warnke, R., Holden, J.T., Bray, R.A., Waller, E.K. and Buck, D.W.

Notes: Promega's Access RT-PCR system, Taq DNA Polymerase and PolyATtract® mRNA Isolation System were used in this study. (1991)

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Proc. Natl. Acad. Sci. USA 94, 4149-4154. Target-independent cholinergic differentiation in the rat sympathetic nervous system. 1997

Schäfer, M., Schütz, B., Weihe, E. and Eiden, L.

Notes: The pGEM®-T Vector System was used to clone a 270bp RT-PCR product from rat spinal cord mRNA.. (2007)

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Proc. Natl. Acad. Sci. USA 94(7), 3206-3210. Cloning and characterization of the extreme 5'-terminal sequences of the RNA genomes of GB virus C/hepatitis G virus. 1997

Hsieh, S. Y., Yang, P. Y., Chen, H. C., and Liaw, Y. F.

Notes: The RNasin® Ribonuclease Inhibitor and the pGEM®-T Vector System were used in this study. The inhibitor was used to protect RNA during the T4 RNA ligase step for 5' RACE analysis. (1643)

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Immunity 6, 119-129. cDNA cloning and primary structure analysis of C1qR(P), the human C1q/MBL/SPA receptor that mediates enhanced phagocytosis in vitro. 1997

Nepomuceno, R.R., Henschen Edman, A.H., Burgess, W.H., Tenner, A.J.

Notes: RT-PCR was performed with degenerate primers and the resulting 110bp product was subcloned with the pGEM®-T Vector System. The 110bp fragment was used to screen a cDNA library and four positive plaques were identified. (0650)

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J. Biol. Chem. 272, 13019-13025. TrkB variants with deletions in the leucine-rich motifs of the extracellular domain. 1997

Ninkina, N. , Grashchuck, M. , Buchman, V. L. , Davies, A. M.

Notes: The PolyATtract® System 1000 was used to isolate polyA+ RNA directly from newborn mouse brains. The RNA was used in RT-PCR for detection of specific TrkB variants. Taq DNA Polymerase and the pGEM®-T Vector System were used to produce and subclone novel TrkB variants that differ in the extracellular domain. The CellTiter 96® Non-Radioactive Cell Proliferation Assay was performed on NIH 3T3 cells transfected with the TrkB receptors and challenged with BDNF, NT3 and NT4/5. (0615)

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J. Neurosci. 17, 6094-6104.. Detection of functional nicotinic receptors blocked by alpha-bungarotoxin on PC12 cells and dependence of their expression on post-translational events. 1997

Blumenthal, E.M., Conroy, W.G., Romano, S.J., Kassner, P. D. and Berg, D. K.

Notes: NGF was used to induce PC12 cell differentiation 3-5 days prior to assay. PCR amplimers representing various subunits of the acetylcholine receptor were subcloned into the pGEM®-T Vector. The resulting clones were used to produce RNA probes for RNase protection assays. Also, a chimeric a5/7-HT3 receptor was generated by PCR, subcloned into the pGEM®-T Vector and sequenced. (1419)

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J. Neurosci. 17, 8156-8168. Nervous system-specific expression of a novel serine protease: Regulation in the adult rat spinal cord by excitotoxic injury 1997

Scarisbrick, I.A., Towner, M.D., Isackson, P.J.

Notes: PolyA+ RNA was isolated from rat total RNA using the PolyATtract® mRNA Isolation System and used for Northern analysis. The pGEM®-T Vector System was used to clone products from RT-PCR. (0447)

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J. Biol. Chem. 272, 9464-9473. Intracellular Ca2+ pool content and signaling and expression of a calcium pump are linked to virulence in Leishmania mexicana amazonesis amastigotes. 1997

Lu, H.G., Zhong, L., Chang, K.P., Docampo, R.

Notes: PolyATtract® mRNA Isolation System was used to isolate Poly A+ RNA from the protozoan Leishmania mexicanna amazonesis total RNA. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM®-T Vector. (0748)

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J. Biol. Chem. 272, 18842-18848. Molecular cloning of a novel polypeptide, DP5, induced during programmed neuronal death. 1997

Imaizumi, K., Tsuda, M., Imai, Y., Wanaka, A., Takagi, T., Tohyama, M.

Notes: Superior cervical ganglia were maintained in culture with or without the 2.5S NGF. RNA was isolated from these cultures and used for differential display with RT-PCR. The RT-PCR products were cloned with the pGEM®-T Vector System. (0998)

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