Notes: Rat hippocampal neurons were used to demonstrate that vasoactive intestinal polypeptide promotes neuronal differentiation through activity-dependent neurotrophic factor (ADNF) which causes secretion of neurotrophin 3. NT-3 levels in the cultured medium were quantitated using Promega's NT-3 E_max® ImmunoAssay System. (2338)

Notes: TGFβ2 and proinflammatory cytokine levels were determined in serum and in the aqueous humor of mice afflicted with experimental autoimmune uveitis. Aqueous humor samples from at least five mice were pooled, centrifuged at 3000rpm for 3 minutes, and the cell-free supernatant was frozen immediately at  –70°C. TGFβ2 was quantitated using Promega's TGFβ2 E_max® ImmunoAssay System. (2347)

Notes: The secretion of TGFβ2 in monocultures and cocultures of rat mesenchymal peritubular and epithelial Sertoli cells was examined. Treatment with follicle stimulating hormone revealed a reduced secretion of TGFβ2 in cocultures. Total TGFβ2 was quantitated by acid activation of the conditioned media with HCl at pH 2.0–3.0 for 15 minutes followed by neutralization with NaOH to approximately pH 7.6. Bioactive TGFβ2 levels were determined without acid activation. TGFβ2 levels were determined using Promega's TGFβ2 E_max® ImmunoAssay System. (2349)

Notes: The TGFβ2 E_max^® ImmunoAssay System (TGFβ2 ELISA) was used to determine the amount of TGF-β2 secreted from cultured splenocytes isolated for Listeria-infected mice. Splenocytes obtained from anti-CD1-treated mice secreted less TGFβ2 than control IgG-treated mice. (0290)

Notes: Rats were fed a 8.0% NaCl diet; aorta explants were taken from these rats and levels TGFβ1 were measured using Promega's TGFβ1 E_max® ImmunoAssay System. Both total and active TGF beta1 levels increased. The Prime-a-Gene® Labeling System was used to make radiolabeled DNA probes. (2340)

Notes: Mice were injected with murine ovarian teratocarcinoma cells to form peritoneal tumors as a model for advanced ovarian cancer. The concentration of numerous cytokines, including naturally processed (not acid-activated in vitro) TGFβ2 in the ascites was determined using Promega's TGFβ2 E_max® Immunoassay System. The ascites samples were spun at 14,000 rpm for 2 minutes and the supernatant was assayed. (2348)

Notes: The TGFβ₂ E_max^® ImmunoAssay System was used to monitor TGF-β₂ secreted from mixed cultures containing mouse T cells and allogenic or syngenic microglia or peritoneal exudate cells. The level of the factor was examined every 24 hours through a 96-hour period. The greatest secretion of TGFβ₂ (~225pg/ml) from 48-hour cultures of T cell and syngenic microglia. (0759)

Notes: The NT-3 Emax ImmunoAssay System was used to determine NT-3 levels in mouse muscle and serum from animals expressing NT-3 from an adenoviral vector. Good detail is given for muscle tissue extraction. (1576)

Notes: The GDNF E_max ImmunoAssay System was used to quantify the level of GDNF in the conditioned media of HeLa cells transduced with a recombinant adenovirus expressing GDNF. The RQ1 DNase was used to treat RNA sample to remove genomic DNA and the AMV reverse transcriptase was used for first strand cDNA synthesis. (1605)