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Nucl. Acids Res. 31 (7), e35. Light-directed 5´-->3´ synthesis of complex oligonucleotide microarrays. 2003

Albert, T.J., Norton, J., Ott, M., Richmond, T., Nuwaysir, K., Nuwaysir, E.F., Stengele, K.P. and Green, R.D.

Notes: The PolyATtract® System 1000 was used to isolate mRNA from mouse spleen. The researchers used 3μg of the isolated poly(A)+ RNA in reverse transcriptase reactions to make cDNAs. The cDNAs were ultimately used as templates in in vitro transcription reactions to produce biotin labeled cRNAs that were used as targets in microarray analysis experiments.  (2743)

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Genes Dev. 14, 2015-2027. PML is induced by oncogenic ras and promotes premature senescence. 2000

Ferbeyre G., de Stanchina E., Querido E., Baptiste N., Prives C. and Lowe S.W.

Notes: The PolyATtract® System 1000 was used to isolated polyA+ RNA directly from IMR90 cells infected with retroviruses expressing the oncogenic ras or an empty vector control. The isolated polyA+ RNA was used as template in RT-PCR reactions containing 33P-αdCTP. The radiolabeled targets were then hybridized to microarrays (Genome Discoveries) for differential analysis of IMR90 fibroblast gene expression with the ras oncogene expressing retrovirus or an empty retrovirus control. (2695)

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Brain Res. Mol. Brain Res. 67, 46-52.. Cultures of astrocytes and microglia express interleukin 18. 1999

Conti, B., Park, L.C.H., Calingasan, N.Y., Kim, Y., Kim, H., Bae, Y., Gibson, G.E., Joh, T.H.

Notes: The PolyATtract® System 1000 was used to isolate polyA+ RNA directly from cultures of primary mouse astrocytes and the mouse microglia cell line BV-2. The isolated RNA was used for Northern analysis. (1275)

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Biol. Reprod. 60, 322-329. Effect of oxytocin receptor and beta2-adrenoceptor blockade on myometrial oxytocin receptors in parturient rats. 1999

Engstrom, T. , Bratholm, P. , Vilhardt, H. , Christensen, N.J.

Notes: Authors use the PolyATtract® 1000 System to isolate mRNA from rat myometrium and use this in quantitative RT-PCR experiments. (1220)

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Stem Cells 17, 121-124. Expression of platelet-activating factor receptor transcript-1 but not transcript-2 by human bone marrow cells 1999

Desplat, V., Besse, A., Faucher, J.L., Praloran, V., Denizot, Y.

Notes: The PolyATtract® System 1000 was used to isolate poly(A)+ RNA directly from 5637 human bladder cacinoma cells. The isolated RNA was used for RT-PCR. (1272)

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J. Biol. Chem. 274, 3017-3025. Isolation, cloning, and characterization of a new mammalian coronin family member, coroninse, which is regulated within the protein kinase C signaling pathway. 1999

Parente, J. A., Jr., Chen, X., Zhou, C., Petropoulos, A.C., Chew, C.S.

Notes: These authors used Promega's PolyATtract® System 1000 to isolate mRNA from rabbit primary parietal cells. (0585)

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J. Virol. 73, 8559-8570. Mutations within the Autographa californica nucleopolyhedrovirus FP25K gene decrease the accumulation of ODV-E66 and alter its intranuclear transport. 1999

Braunagel, S.C., Burks, J.K., Rosas-Acosta, G., Harrison, R.L., Ma, H., Summers, M.D.

Notes: Sf9 Spodoptera frugiperda cells were infected with the AcMNPV virus. Poly(A)+ RNA was isolated directly from the cells using the PolyATtract® System 1000. The isolated RNA was used for cDNA library construction. (1401)

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J. Virol. 73, 2376-2384. Myxoma virus encodes an α2,3-sialyltransferase that enhances virulence. 1999

Jackson, R.J., Hall, D.F., Kerr, P.J.

Notes: The PolyATtract® System 1000 was used to isolate poly(A)+ RNA directly from myxoma virus-infected RK13 rabbit kidney cells. To obtain late viral RNAs, the RNA was isolated from the cells 16 hours after infection. For the early viral RNAs, the cells were treated with cycloheximide for 10 hours after infection prior to RNA isolation. The isolated RNA was used for Northern blot analysis with RNA probes prepared using the Riboprobe® System. (0966)

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J. Biol. Chem. 274, 37340-37378. The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1 1999

Singh, B.B., Patel, H.H., Roepman, R., Schick, D., Ferreira, P.A.

Notes: The PolyATtract® System 1000 was used to isolate poly A+ RNA directly from bovine retinas. The isolated RNA was used for RT-PCR amplification. (0369)

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Am. J. Physiol. 275, C56-C67. Lasp-1 is a regulated phosphoprotein within the cAMP signaling pathway in the gastric parietal cell. 1998

Chew, C.S., Parente, J.A. Jr, Zhou, C., Baranco, E. and Chen, X.

Notes: In this paper, the PolyATtract® System 1000 was used to isolate mRNA from gastric mucosae cells isolated from rabbits. The mRNA was used for 3' RACE. (2010)

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Oncogene 16(2), 249-255. Mutations of the p53 gene in canine lymphoma and evidence for germ line p53 mutations in the dog. 1998

Veldhoen, N., Stewart, J., Brown, R. and Milner, J.

Notes: The PolyATtract® System 1000 was used to isolate polyA+ RNA directly from peripheral blood leukocytes. A 30µl preparation of mRNA was isolated from 1.2 x109 cells. Three microliters of the isolated RNA was used for RT-PCR amplification of the p53 message with the Access RT-PCR System. (1656)

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J. Immunol. 161, 474-480. System administration of endotoxin induces bronchopulmonary hyperactivity dissociated from TNF-alpha formation and neutrophil sequestration into the murine lungs. 1998

Lefort, J., Singer, M., Leduc, D., Renesto, P., Nahori, M.A., Huerre, M., Créminon, Chignard, M., Vargaftig, B.B.

Notes: Poly A+ RNA was isolated directly from PBS-perfused mouse lungs with the PolyATtract® System 1000. The isolate poly A+ RNA was used for quantitative two-step RT-PCR using M-MLV RNase Hminus Reverse Transcriptase as well as RNasin® Ribonuclease inhibitor for first step cDNA synthesis. (0821)

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Am. J. Physiol. 275, L288-L293. Thioredoxin overexpression prevents NO-induced reduction of NO synthase activity in lung endothelial cells. 1998

Zhang, J. , Li, Y. D. , Patel, J. M. , Block, E. R.

Notes: The PolyATtract® System 1000 is used to isolation polyA+ mRNA from pig main pulmonary artery endothelial cells. The isolated mRNA is used for non-radioactive Northern blots. (0090)

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J. Biol. Chem. 272(4), 2035-2037. Induction of interferon-γ inducing factor in the adrenal cortex. 1997

Conti, B., Jahng, J.W., Tinti, C., Son, J.H. and Joh, T.H.

Notes: The PolyATtract® System 1000 was used to isolate poly A+ RNA directly from the adrenal glands of rats treated with reserpine and from nontreated controls. The isolated poly A+ RNA was used for differential display RT-PCR (1652)

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J. Biol. Chem. 272, 2035-2037.. Induction of interferon-gamma inducing factor in the adrenal cortex. 1997

Conti, B. , Jahng, J. W. , Tinti, C. , Son, J. H. , Joh, T. H.

Notes: Poly(A)+ RNA was isolated directly from differentially treated rat adrenal glands using the PolyATtract® System 1000 and used for reverse transcription and differential display analysis. (1274)

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Proc. Natl. Acad. Sci. USA 94, 8907-11. Mendel's dwarfing gene: cDNAs from the Le alleles and function of the expressed proteins. 1997

Martin, D.N., Proebsting, W.M., Hedden, P.

Notes: The PolyATtract® System 1000 was used to isolate polyA+ RNA from Pisium sp. plant tissue. Many details of the isolation are given in the paper. The isolated polyA+ RNA was used for RT-PCR and Northern blot analyses. (0736)

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Proc. Natl. Acad. Sci. USA 94(16), 8812-8817. Transcription initiation is controlled by three core promoter elements in the hgl5 gene of the protozoan parasite Entamoeba histolytica. 1997

Singh, U., Rogers, J.B., Mann, B.J. and Petri, W.A., Jr.

Notes: Poly A+ RNA was isolated directly from E. histolytica using the PolyATtract® System 1000 and used for Northern and primer extension analysis. The RNAgents® Total RNA Isolation System was used to isolate total RNA from E. histolytica. The isolated RNA was used for nuclear run-on transcription. (1654)

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J. Biol. Chem. 272, 13019-13025. TrkB variants with deletions in the leucine-rich motifs of the extracellular domain. 1997

Ninkina, N. , Grashchuck, M. , Buchman, V. L. , Davies, A. M.

Notes: The PolyATtract® System 1000 was used to isolate polyA+ RNA directly from newborn mouse brains. The RNA was used in RT-PCR for detection of specific TrkB variants. Taq DNA Polymerase and the pGEM®-T Vector System were used to produce and subclone novel TrkB variants that differ in the extracellular domain. The CellTiter 96® Non-Radioactive Cell Proliferation Assay was performed on NIH 3T3 cells transfected with the TrkB receptors and challenged with BDNF, NT3 and NT4/5. (0615)

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Proc. Natl. Acad. Sci. USA 93(23), 12920-12925. Complementation of methylation deficiency in embryonic stem cells by a DNA methyltransferase minigene. 1996

Tucker, K.L., Talbot, D., Lee, M.A., Leonhardt, H. and Jaenisch, R.

Notes: Poly A+ RNA was isolated directly from mouse kidney using PolyATtract® System 1000 and used in 5’-RACE analysis. (1655)

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