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Circ. Res. 83, 508-515. Lysophosphatidylcholine enhances cytokine-induced interferon gamma expression in human T lymphocytes. 1998

Nishi, E., Kume, N., Ueno, Y., Ochi, H., Moriwaki, H., Kita, T.

Notes: Promoter studies were performed in human peripheral T lymphocytes. Experimental constructs in the pGL2-Basic Vector and cotransfected with the pRL-TK Vector at a 20:1 ratio. The luciferases were detected with the Dual-Luciferase® Reporter Assay System. (0616)

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Proc. Natl. Acad. Sci. USA 95, 3650. Packaging of intron-containing genes into retrovirus vectors by alphavirus vectors. 1998

Li, K.-J., Garoff, H.

Notes: To assess the ability of a cytoplasmic Semliki Forest virus (SFv) expression system to generate an RNA for packaging containing an intron, the promoter, intron, CAT gene and poly A signal of the pCAT® Control Vector was inserted into a SFv vector. The SFv sequences and the CAT sequences were transcribed in vitro and co-transfected into BHK-21 cells with SFv vectors containing the Moloney murine leukemia virus packaging proteins. This method produced retrovirus particles that were of high titer and were used to infect NIH 3T3 cells. The CAT gene expression in the NIH 3T3 cells was measured with the CAT Enzyme Assay System. The presence of the intron in the infected cells was confirmed by isolating total RNA and performing RT-PCR with primers that could distinguish whether or not the cells got the CAT gene with the intron. RT-PCR was performed with the Access RT-PCR System. (0795)

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J. Biol. Chem. 273, 27474-27483. Sp1 and Sp3 regulate transcriptional activity of the facilitative glucose transporter isoform-3 gene in mammalian neuroblasts and trophoblasts. 1998

Rajakumar, R.A., Thamotharan, S., Menon, R.K., Devaskar, S.U.

Notes: AMV Reverse Transcriptase was used for primer extension analysis from poly(A)+ RNA. The Riboprobe® in vitro Transcription System was used to generate [32P]-antisense RNA probes for RNase protection assays. Promoter studies were performed in N2A murine neuroblasotoma cells. Experimental constructs were assembled in the pGL2-Basic Vector and cotransfected with the pRL-TK Vector at a 10:1 ratio and luciferase activities determined with the Dual-Luciferase® Reporter Assay System. Some promoter studies were performed in Drosophila Schneider cells with luciferase promoter constructs and an RSV-driven beta-galactosidase vector. Beta-Galactosidase activity was determined with the Beta-Galactosidase Enzyme Assay System. (0531)

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J. Biol. Chem. 273, 26218-26224. Synergistic activation of the N-methyl-D-aspartate receptor subunit 1 promoter by myocyte enhancer factor 2C and Sp1. 1998

Krainc, D., Bai, G., Okamoto, S., Carles, M., Kusiak, J.W., Brent, R.N., Lipton, S.A.

Notes: Luciferase (prepared in pGL2-Basic Vector) and β-galactosidase (pSV-β-Galactosidase Control Vector) reporters were studied in SL2 Drosophila cells, HeLa cells and primary rat cortical neurons. Transfections into the SL2 and HeLa cells were accomplished with standard calcium phosphate methods. Transfection of primary rat cortical neurons was accomplished with the Tfx™-50 Reagent as described in Boeckman, F.A. et al. (1996) Neural Notes II(1), 13-15. Reporter assays were performed with the Luciferase Assay System and the β-Galactosidase Assay System. (0890)

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J. Biol. Chem. 273, 21137-21144. Transcriptional activation of the p21WAF1, CIP1, SDI1 gene by interleukin-6 type cytokines: A prerequisite for their pro-differentiating and anti-apoptotic effects on human osteoblastic cells. 1998

Bellido, T., O'Brien, C.A., Roberson, P.K. and Manolagas, S.C.

Notes: Studies were performed in MG63 human osteosarcoma cells. Firefly luciferase activity was normalized to Renilla luciferase activity using the Dual-Luciferase® Reporter Assay System. The cells were transfected with the pGL2-Basic Vector containing the experimental promoters, pRL-CMV Vector, and a Stat-3 expression vector. The ratio of pGL2 Vector to pRL-CMV Vector was 1000:1. The ratio of pGL2 Vector to Stat3 Expression Vector was 6:1. The chemical MTS was used in a LDH-coupled assay to determine cell viability of osteosarcoma cells transfected with antisense oligonucleotides. (1436)

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J. Neurosci. 17, 2273-2283. β43´: an enhancer displaying neural-restricted activity is located in the 3´-untranslated exon of the rat nicotinic acetylcholine receptor β4 gene. 1997

McDonough, J., Deneris, E.

Notes: Reporter studies were performed mainly in PC12 cells pGL2-Basic, pGL2-Promoter and pGL2-Control Vectors. Neuronal specificity was assayed in PC12, HeLa, Rat2 (rat fibroblast), Clone 9 (rat liver), ARIP (rat pancreatic tumor), C1300 (rat neuroblastoma) and rat keratinocytes. Luciferase activity was monitored with the the Luciferase Assay System. (0710)

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J. Biol. Chem. 272, 18490-18497.  Two distinct factor-binding DNA elements in cardiac myosin light chain 2 gene are essential for repression of its expression in skeletal muscle.  Isolation of a cDNA clone for repressor protein Nished. 1997

Dhar, M. , Mascareno, E.M. , Siddiqui, M.A.Q.

Notes: Luciferase studies were performed in primary chicken skeletal muscle cells using the Luciferase Assay System. Experimental constructs were prepared in the pGL2 Basic and pGL2 Promoter Vectors. (1229)

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J. Biol. Chem. 272, 22667-22678. A minimal murine Msx-1 gene promoter. organization of its cis-regulatory motifs and their role in transcriptional activation in cells in culture and in transgenic mice. 1997

Takahashi, T., Guron, C., Shetty, S., Matsui, H., Raghow, R.

Notes: Expression of firefly luciferase gene fused with the promoter of interest was performed in NIH3T3 and C2C12 myoblasts. The Altered Sites® II in vitro Mutagenesis System was used to produce site-directed mutations within the promoter. (0294)

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J. Biol. Chem. 272, 17485-17494. Androgenic Induction of Prostate-specific Antigen Gene Is Repressed by Protein-Protein Interaction between the Androgen Receptor and AP-1/c- Jun in the Human Prostate Cancer Cell Line LNCaP 1997

Sato, N., Sadar, M.D., Bruchovsky, N., Saatcioglu, F., Rennie, P.S., Sato, S., Lange, P.H., Gleave, M.E.

Notes: Luciferase studies were performed in the human prostate cell line, LNCap, using constructs prepared in the pGL2 Basic Vector. (0441)

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Proc. Natl. Acad. Sci. USA 94, 7543-7548. Angiotensin II type1a receptor gene expression in the heart: AP-1 and GATA-4 participate in the response to pressure overload. 1997

Herzig, T.C., Jobe, S.M., Aoki, H., Molkentin, J.D., Cowley, A.W., Jr., Izumo, S., Markham, B.E.

Notes: Luciferase studies were performed in primary rat cardiac ventricular myocytes using constructs prepared in the pGL2-Basic Vector. (1041)

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J. Biol. Chem. 272, 1753-1760. Binding of upstream stimulatory factor to an E-box in the 3'-flanking region stimulates alpha1(I) collagen gene transcription. 1997

Rippe, R.A., Umezawa, A., Kimball, J.P., Breindl, M., Brenner, D.A.

Notes: Promoter analysis studies using the a1(I) collagen promoter were performed using the pGL2 Basic Vector. (0477)

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J. Biol. Chem. 272, 22199-22206. Butyrate activates the WAF1/Cip1 gene promoter through Sp1 sites in a p53-negative human colon cancer cell line. 1997

Nakano, K., Mizuno, T., Sowa, Y., Orita, T., Yoshino, T., Okuyama, Y., Fujita, T., Ohtani-Fujita, N., Matsukawa, Y., Tokino, T., Yamagishi, H., Oka, T., Nomura, H., Sakai, T.

Notes: Reporter studies were performed in WiDR human adenocarcinoma cells and MG63 human osteosarcoma cells. Experimental constructs were prepared in either the pGL2 Basic Vector or the pGL3 Basic Vector. (0644)

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Blood 90, 2784-2795. CCAAT displacement protein (CDP/cut) recognizes a silencer element within the lactoferrin gene promoter. 1997

Khanna-Gupta, A., Zibello, T., Kolla, S., Neufeld, E.J., Berliner, N.

Notes: Luciferase reporter studies were performed in K562 and HEL human erythroleukemia cells using constructs prepared in the pGL2-Basic Vector. (0936)

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J. Biol. Chem. 272, 31793-31800. CCAAT/Enhancer-binding protein delta activates insulin-like growth factor-I gene transcription in osteoblasts. Identification Of a novel cyclic amp signaling pathway in bone 1997

Umayahara, Y., Ji, C., Centrella, M., Rotwein, P., McCarthy, T.L.

Notes: Studies were performed in COS-7 cells. The experimental firefly luciferase vector (derived from pGL2-Basic Vector) was co-transfected with the Renilla control Vector (pRL-CMV Vector) and plasmids expressing either the CCAAT/Enhancer-binding protein beta or delta from a CMV promoter. The firefly luciferase:expression plasmid ratio was 10:1 and the firefly luciferase:Renilla luciferase vector ratio was 1000:1. In vitro translations were performed with the TNT® Coupled Reticulocyte Lysate System. (0252)

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J. Biol. Chem. 272, 9166-9174. Cell cycle regulation of the human polo-like kinase (PLK) promoter. 1997

Uchiumi, T., Longo, D.L., Ferris, D.K.

Notes: The authors study transient and stable expression of several luciferase reporter constructs. The PLK promoter-luciferase fusion gene constructs using the pGL2 -Basic Vector were transfected into HeLa cells. The reporter construct and the pRSV-neo vector were co-transfected for production of the stable transfectants. (0249)

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J. Biol. Chem. 272, 17438-17443. Cell-specific expression of the glucose-dependent insulinotropic polypeptide gene in a mouse neuroendocrine tumor cell line. 1997

Boylan, M. O. , Jepeal, L. I. , Jarboe, L. A. , Wolfe, M. M.

Notes: Luciferase studies were performed in STC-1 mouse endocrine cells using constructs prepared in the pGL2 Basic Vector. (1396)

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J. Biol. Chem. 272, 8581-8593. Characterization of a subset of the basic-helix-loop-helix-PAS superfamily that interacts with components of the dioxin signaling pathway. 1997

Hogenesch, J.B., Chan, W.K., Jackiw, V.H., Brown, R.C., Gu, Y.Z., Pray-Grant, M., Perdew, G.H., Bradfield, C.A.

Notes: Luciferase studies were performed in Hep3b cells. Experimental constructs were prepared in the pGL2-Promoter Vector and luciferase activities determined with the Luciferase Assay System. (1052)

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J. Biol. Chem. 272, 12809-12815.. Characterization of retinoic acid-induced AP-1 activity in B16 mouse melanoma cells. 1997

Desai, S. H. , Niles, R. M.

Notes: In this paper, the pGL2 Basic Vector, the pSV-Beta Galactosidase Control Vector and the Luciferase Assay System were used to study gene expression in B16 mouse melanoma cells. (1271)

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J. Biol. Chem. 272, 14365-14371. cis-Acting elements and trans-acting proteins in the transcriptional inhibition of gonadotropin-releasing hormone gene by human chorionic gonadotropin in immortalized hypothalamic GT1-7 neurons. 1997

Lei, Z. , Rao, C. V.

Notes: Luciferase studies were performed in GT1-7 immortalized neurons using construct assembled in the pGL2 Basic Vector. Luciferase activity was measured with the Luciferase Assay System. (0823)

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J. Neurosci. 17, 4159-4169. Cloning and functional characterization of Roaz, a zinc finger protein that interacts with O/E-1 to regulate gene expression: implications for olfactory neuronal development. 1997

Tsai, R. Y. , Reed, R. R.

Notes: Reporter studies were performed in human embryonic kidney 293 cells using constructs prepared in the pGL2-Basic Vector. The Erase-A-Base® System was used to create unidirectional deletion mutants of promoter elements. (0236)

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J. Biol. Chem. 272, 21616-21624. Cooperative binding of NF-Y and Sp1 at the DNase I-hypersensitive site, fatty acid synthase insulin-responsive element 1, located at -500 in the rat fatty acid synthase promoter 1997

Roder, K., Wolf, S.S., Beck, K.F., Schweizer, M.

Notes: Luciferase studies were performed on extracts of transfected HepG2 cells using constructs prepared in the pGL2 Basic Vector. (0486)

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J. Biol. Chem. 272, 1904-1909. Cytoskeleton reorganization induces the urokinase-type plasminogen activator gene via the Ras/extracellular signal-regulated kinase (ERK) signaling pathway. 1997

Irigoyen, J.P., Besser, D., Nagamine, Y.

Notes: Luciferase studies were performed in LLC-PK1 nontransformed renal epithelial cells. Reporter constructs were prepared in the pGL2-Basic Vector. The pGL2-Control Vector was used as well. (1002)

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EMBO J. 16, 406-416. Deficient cytokine signaling in mouse embryo fibroblasts with a targeted deletion in the PKR gene: role of IRF-1 and NF-kappaB. 1997

Kumar, A., Yang, Y.L., Flati, V., Der, S., Kadereit, S., Deb, A., Haque, J., Reis, L., Weissmann, C., Williams, B.R.

Notes: Luciferase studies were performed in mouse embryo fibroblasts. Experimental constructs were prepared in the pGL2-Basic Vector and luciferase activities determined with the Luciferase Assay System. (0861)

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J. Biol. Chem. 272, 7464-7472. Differential effects of protein kinase C, Ras, and Raf-1 kinase on the induction of the cardiac B-type natriuretic peptide gene through a critical promoter-proximal M-CAT element. 1997

Thuerauf, D.J., Glembotski, C.C.

Notes: Luciferase studies were performed in primary neonatal rat ventricular myocardiocytes and constructs were prepared in the pGL2-Basic Vector. The promoter mutations of the reporter constructs were made by Altered Sites® II in vitro Mutagenesis System. (0264)

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J. Neurosci. 17, 6554-6564.. Differential expression of alpha-bungarotoxin-sensitive neuronal nicotinic receptors in adrenergic chromaffin cells: A role for transcription factor Egr-1. 1997

Criado, M., Dominguez del Toro, E.D., Carrasco-Serrano, C., Smillie, F.I., Juíz, J.M., Viniegra, S. and Ballesta, J.J.

Notes: Total RNA was isolated from adrenomedullary tissue dissected from areas near to or far from the adrenal cortex. The isolated RNA was used for RT-PCR. Reporter studies were performed in Neuro2a murine neuroblastomas, SH-SY5Y human neuroblastomas and bovine chromaffin cells. All luciferase activities were normalized to control beta-Galactosidase activity. The consensus oligonucleotides were used in gel shift assays of bovine chromaffin cell nuclear extracts. The oligonucleotides were used to show specificity for Egr-1. (1614)

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