Hoeren, F.U., Dolferus, R., Wu, Y., Peacock, W.J., Dennis, E.S.
Notes: Quantitative RT-PCR was carried out using 1µg total RNA from Arabidopsis using Access RT-PCR System. Samples were taken during the PCR reaction after 5, 10, 15, and 25 cycles and loaded onto agarose gels. Gels were treated for Southern blot hybridization, and filters were hybridized using the AtMYB2 cDNA. Linearity of signal strength was verified using phosphorimaging system quantifications. (1048)