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J. Biol. Chem. 273, 11556-11562. cDNA cloning and expression of bovine UDP-N-acetylglucosamine: alpha1,3-D-mannoside beta1,4-N-acetyl glucosaminyltransferase IV. 1998

Minowa, M.T., Oguri, S., Yoshida, A., Hara, T., Iwamatsu, A., Ikenaga, H. , Takeuchi, M.

Notes: The Access RT-PCR System was used to compare the abundance of GnT-IV mRNA in various bovine tissues. (0694)

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J. Clin. Microbiol. 36, 1741-1745. Evaluation of a commercially available reverse transcription-PCR assay for diagnosis of enteroviral infection in archival and prospectively collected cerebrospinal fluid specimens 1998

Pozo, F., Casas, I., Tenorio, A., Trallero, G., Echevarria, J.M.

Notes: The authors compared a commercially available enterovirus-specific RT-PCR kit with a kit of their own design. Their 'in-house' RT-PCR system used the Access RT-PCR System to amplify enterovirus sequences from cerebral spinal fluid extracts followed by nested-PCR for final amplification of the target. Both the commercial system and the 'in-house' system were adequate for amplification of enterovirus sequences from CSF of infected individuals. Other, more time-consuming assays were performed to verify the results from the RT-PCR tests. (0545)

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Genetics 149, 479-490. Evidence for a role for AtMYB2 in the induction of the Arabidopsis alcohol dehydrogenase gene (ADH1) by low oxygen. 1998

Hoeren, F.U., Dolferus, R., Wu, Y., Peacock, W.J., Dennis, E.S.

Notes: Quantitative RT-PCR was carried out using 1µg total RNA from Arabidopsis using Access RT-PCR System. Samples were taken during the PCR reaction after 5, 10, 15, and 25 cycles and loaded onto agarose gels. Gels were treated for Southern blot hybridization, and filters were hybridized using the AtMYB2 cDNA. Linearity of signal strength was verified using phosphorimaging system quantifications. (1048)

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Proc. Natl. Acad. Sci. USA 95, 8147-8152. Evolution of the avian sex chromosomes from an ancestral pair of autosomes. 1998

Fridolfsson, A.-K., Cheng, H., Copeland, N.G., Jenkins, N.A., Liu, H.-C., Raudsepp, T., Woodage, T., Chowdhary, B., Halverson, J., Ellegren, H.

Notes: The Access RT-PCR System was used to amplify two portions of the CHD1 gene product from ostrich poly A+ RNA. The amplification products were gel purified and sequenced. The sequence obtained was compared to that of the human and murine CHD1 and Chicken CHD1Z and CHD1W sequences. (1175)

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J. Invest. Dermatol. 111, 1053–1057. Expression of interleukin-12 is increased in psoriatic skin. 1998

Yawalker, N., Karlen, S., Hunger, R., Brand, C.U. and Braathen, L.R.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from normal skin, psoriatic skin, H-128 small cell lung carcinoma cells and peripheral blood mononuclear cells. The isolated RNA was used for RT-PCR performed with the Access RT-PCR System. (0107)

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Proc. Natl. Acad. Sci. USA 95, 11476-11481. Hot spots in cold adaptation: Localized increases in conformational flexibility in lactate dehydrogenase A4 orthologs of Antarctic notothenioid fishes. 1998

Fields, P.A., Somero, G.N.

Notes: The Access RT-PCR System was used to amplify the lactate dehydrogenase gene from a variety of Antarctic notothenioid fish. (1154)

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Infect. Immun. 65, 5131-5136. Induction of neutrophil chemoattractant cytokines by Mycoplasma hominis in alveolar type II cells. 1998

Kruger, T., Baier, J.

Notes: The Access RT-PCR System was used to assess IL-8 (interleukin-8) and ENA-78 (epithelial cell-derived neutrophil-activating peptide) mRNA in M. hominis-infected and untreated A549 cells. (0894)

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Oncogene 16(2), 249-255. Mutations of the p53 gene in canine lymphoma and evidence for germ line p53 mutations in the dog. 1998

Veldhoen, N., Stewart, J., Brown, R. and Milner, J.

Notes: The PolyATtract® System 1000 was used to isolate polyA+ RNA directly from peripheral blood leukocytes. A 30µl preparation of mRNA was isolated from 1.2 x109 cells. Three microliters of the isolated RNA was used for RT-PCR amplification of the p53 message with the Access RT-PCR System. (1656)

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J. Bacteriol. 180, 2043-2049. ntn genes determining the early steps in the divergent catabolism of 4-nitrotoluene and toluene in Pseudomonas sp. Strain TW3. 1998

James, K.D., Williams, P.A.

Notes: The Access RT-PCR System was used to study expression of three ntn genes from TW3 cells grown in either 4-nitrotoluene, toluene or succinate. The pET-5a Vector (discontinued) was used to express the ntnC protein in E. coli strain BL21(DE3) pLysS. (0968)

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Proc. Natl. Acad. Sci. USA 95, 3650. Packaging of intron-containing genes into retrovirus vectors by alphavirus vectors. 1998

Li, K.-J., Garoff, H.

Notes: To assess the ability of a cytoplasmic Semliki Forest virus (SFv) expression system to generate an RNA for packaging containing an intron, the promoter, intron, CAT gene and poly A signal of the pCAT® Control Vector was inserted into a SFv vector. The SFv sequences and the CAT sequences were transcribed in vitro and co-transfected into BHK-21 cells with SFv vectors containing the Moloney murine leukemia virus packaging proteins. This method produced retrovirus particles that were of high titer and were used to infect NIH 3T3 cells. The CAT gene expression in the NIH 3T3 cells was measured with the CAT Enzyme Assay System. The presence of the intron in the infected cells was confirmed by isolating total RNA and performing RT-PCR with primers that could distinguish whether or not the cells got the CAT gene with the intron. RT-PCR was performed with the Access RT-PCR System. (0795)

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J. Biol. Chem. 273, 19378–19382. Restoration of beta1A integrins is required for lysophosphatidic acid-induced migration of beta1-null mouse fibroblastic cells 1998

Sakai, T., Peyruchaud, O., Fässler, R. and Mosher, D.F.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from mouse fibroblasts. The isolated RNA was used for RT-PCR analysis with the Access RT-PCR System. (0474)

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J. Mol. Neurosci. 11, 183-197. Temporal relations among amyloid beta-peptide-induced free-radical oxidative stress, neuronal toxicity, and neuronal defensive responses 1998

Yatin, S.M., Aksenova, M., Aksenov, M., Markesbery, W.R., Aulick, T., Butterfield, D.A.

Notes: The Access RT-PCR System was used for a semi-quantitative assessment of the quantity of Cu/Zn superoxide dismutase (Cu/Zn SOD) Mn superoxide dismutase (Mn SOD) mRNA levels. Primary embryonic hippocampal neurons were treated with Abeta(25-35) for zero to 25 hours. The level of the Cu/Zn SOD and Mn SOD mRNA was related as percent of control, untreated cultures. The PCR portion of the reaction was performed for 22 cycles to insure that the reaction was in the linear range. (0106)

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J. Immunol. 161, 1844-1852. The invariant chain gene intronic enhancer shows homology to Class II promoter elements. 1998

Moore, B.B., Cao, Z.A., McRae, T.L., Woo, C.H., Conley, S., Jones, P.P.

Notes: The Access RT-PCR System was used to assess expression of the CIITA gene in fibroblast L and splenic B cells. The 700bp transcript could be detected with as little as 40ng of poly(A)+ RNA from the splenic B cells but undetectable in as much as 5µg of poly (A)+ RNA from the L cells. Both sources of RNA were readily amplified for a 510bp β-actin message from 5µg down to 40ng. (0657)

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J. Biol. Chem. 273, 13313-13316. Vascular endothelial growth factor induces expression of the antiapoptotic proteins Bcl-2 and A1 in vascular endothelial cells. 1998

Gerber, H.P., Dixit, V., Ferrara, N.

Notes: The Access RT-PCR System was used to study expression of Bcl-2, A1, Bax and Bad genes in HUVE (human umbilical vein endothelial) cells treated with VEGF (vascular endothelial growth factor). The technique of 'real-time' quantitative RT-PCR was employed. (1150)

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Microbiology 94, 6484-6489. A cluster of bacterial genes for anaerobic benzene ring biodegradation. 1997

Egland, P.G., Pelletier, D.A., Marilyn, D.M., Gibson, J. and Harwood, C.S

Notes: The authors used Promega's AMV Primer Extension System and Access RT-PCR System for this study. (2216)

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Blood 90(12), 5013-5502. A novel five-transmembrane hematopoietic stem cell antigen: Isolation, characterization, and molecular cloning. 1997

Miraglia, S., Godfrey, W., Yin, A.H., Atkins, K., Warnke, R., Holden, J.T., Bray, R.A., Waller, E.K. and Buck, D.W.

Notes: Promega's Access RT-PCR system, Taq DNA Polymerase and PolyATtract® mRNA Isolation System were used in this study. (1991)

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J. Clin. Invest. 100(8), 1951-1957. Amelioration of Collagen-induced arthritis by CD95 ( Apo-1/Fas)- ligand Gene Transfer. 1997

Zhang, H., Yang, Y., Horton, J.L., Samoilova, E.B, Judge, T.A., Turka, L.A., Wilson, J.M. and Chen, Y.

Notes: The Access RT-PCR System was used in this study. (1989)

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J. Clin. Invest. 100, 1951-1957. Amelioration of collagen-induced arthritis by CD95 (Apo-1/Fas)-ligand gene transfer 1997

Zhang, H. , Yang, Y. , Horton, J. L. , Samoilova, E. B. , Judge, T. A. , Turka, L. A. , Wilson, J. M. , Chen, Y.

Notes: The Access RT-PCR System was used to amplify the FasL message from total RNA isolated from mice injected with a recombinant adenovirus. The Access RT-PCR System was used as a means to monitor the success of a gene therapy system. The products were analyzed by EtBr-staining and Southern blotting. (0088)

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J. Biol. Chem. 272(5), 3064-3072. ATP-dependent choline phosphate-induced mitogenesis in fibroblasts involves activation of pp70 S6 kinase and phosphatidylinositol 3'-kinase through an extracellular site. Synergistic mitogenic effects of choline phosphate and sphingosine 1-phosphate. 1997

Chung, T., Crilly, K.S., Anderson, W.H., Mukherjee, J.J. and Kiss, Z.

Notes: The Access RT-PCR System was used in this study. (1994)

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J. Biol. Chem. 272, 23659-23667. Differential transcriptional regulation of the two vascular endothelial growth factor receptor genes. 1997

Gerber, H-P., Condorelli F., Park J. and Ferrara N.

Notes: Promega's pGL2 Vectors; Dual-Luciferase® Reporter Assay System and Access RT-PCR System were used in this study. (1992)

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Proc. Natl. Acad. Sci. USA 94, 14536-14541. Evolutionary instability of the major histocompatibility complex class I loci in New World primates. 1997

Cadavid, L.F., Shufflebotham, C., Ruiz, F.J., Yeager, M., Hughes, A.L. and Watkins, D.I.

Notes: The Access RT-PCR System was used in this study. (1988)

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J. Biol. Chem. 272, 18104-18110. Expression of the type II iodothyronine deiodinase in cultured rat astrocytes is selenium-dependent. 1997

Pallud, S., Lennon, A.M., Ramauge, M., Gavaret, J.M., Croteau, W., Pierre, M., Courtin, F. and St. Germain, D.L.

Notes: The Access RT-PCR System was used to monitor the expression of the type II iodothyronine deiodinase in primary rat astrocyte cultures following forskolin treatment. (1592)

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Proc. Natl. Acad. Sci. USA 94, 5137-5140. Phenotypic alterations in insulin-deficient mutant mice. 1997

Duvillie, B., Cordonnier, N., Deltour, L., Dandoy-Dron, F., Itier, J.M., Monthioux, E., Jami, J., Joshi, R.L. and Bucchini, D.

Notes: The Access RT-PCR System was used in this study. (1998)

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Proc. Natl. Acad. Sci. USA 94, 14701-14706. Regulated expression of the diphtheria toxin A chain by a tumor- specific chimeric transcription factor results in selective toxicity for alveolar rhabdomyosarcoma cells. 1997

Massuda, E.S., Dunphy, E.J., Redman, R.A., Schreiber, J.J., Nauta, L.E., Barr, F.G., Maxwell, I.H., Cripe, T.P.

Notes: The Access RT-PCR System was used to demonstrate expression of the transfected cDNAs in cells. The CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) was used to monitor cell survival after diphtheria toxin exposure. (0698)

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Exp. Neurol. 146, 458-465. The expression of creatine kinase isoenzymes in neocortex of patients with neurodegenerative disorders: Alzheimer's and Pick's Disease. 1997

Aksenov, M.Y., Aksenova, M.V., Payne, R.M., Smith, C.D., Markesbery, W.R. and Carney, J.M.

Notes: The authors used Promega's Access RT-PCR System in this study. (2220)

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