Cowles, C.E., Nichols, N.N., and Harwood, C.S.
Notes: The authors described a cluster of 8 genes from P. putida that is thought to be involved in benzoate metabolism: benA, benB, benC, benD, benE, benF, benK, and benR. The transcriptional start site of benA was determined using Promega's Primer Extension System-AMV Reverse Transcriptase. Total RNA used in these primer extension reactions was isolated from P. putida cells using the SV Total RNA Isolation System. The transcriptional organization of the benA, benB, and benC genes was determined by RT-PCR using the Access RT-PCR System. (2301)