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Am. J. Endocrinol. Metab. 288, E731-E740. Expression and secretion of inflammation-related adipokines by human adipocytes differentiated in culture: integrated response to TNFα. 2005

Wang, B., Jenkins, J.R., and Trayhurn, P.

Notes: In this study, the expression of various adipokine genes linked to inflammation was examined during differentiation of pre-adipocytes to adipocytes in primary culture. The effect of TNFα on expression of these adipokines in differentiated human adipocytes was also investigated. qPCR was used to measure expression levels of the various adipokines, including NGF, during development and upon TNFα exposure. In addition, secretion of adipokines into the culture media was measured by ELISA, the NGF Emax® ImmunoAssay System being used to determine NGF levels. (3502)

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Proc. Natl. Acad. Sci. USA 102, 18171-18176. Human stem/progenitor cells from bone marrow promote neurogenesis of endogenous neural stem cells in the hippocampus of mice. 2005

Munoz, J.R., Stoutenger, B.R., Robinson, A.P., Spees, J.L. and Prockrop, D.J.

Notes: In this study, implantation of human bone marrow stem/progenitor cells (MSCs) into the hippocampus of immunodeficient mice was found to stimulate proliferation, migration and differentiation of endogenous neural stem cells. Results of immunohistochemical analyses were confirmed by ELISA. The NGF Emax® Immunoassay System was used to determine NGF levels in isolated hippocampi at various intervals after implantation of the MSCs. (3500)

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Ann. Oncol. 15, 1439–42. Early predictors of peripheral neurotoxicity in cisplatin and paclitaxel combination chemotherapy. 2004

Cavaletti, G., Bogliun, G., Marzorati, L., Zincone, A., Piatti, M., Colombo, N., Franchi, D., La Presa, M.T., Lissoni, A., Buda, A., Fei, F., Cundari, S. and Zanna, C.

Notes: Patients with locally advanced squamous cervical carcinoma were monitored for chemotherapy-induced peripheral neurotoxicity (CIPN). Plasma drawn from the study participants was stored at –80°C prior to testing for nerve growth factor (NGF)levels using the NGF Emax® ImmunoAssay System. The authors note that a highly significant correlation existed between a decrease in circulating levels of NGF and an increase in the severity of CIPN. (3338)

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Biol. Reprod. 68, 2038-2043. Chronic intermittent cold stress activates ovarian sympathetic nerves and modifies ovarian follicular development in the rat. 2003

Dorfman, M., Arancibia, S., Fiedler, J.L. and Lara, H.E.

Notes: The authors studied the effects of a chronic intermittent cold stress regime on sympathetic nerve activation and ovarian physiology. To analyze whether this effect on noradrenaline was preceded by activation of the neurotrophic factor system responsible for growth and survival of sympathetic neurons, they measured nerve growth factor in adult female Sprague-Dawley rats using the NGF Emax® ImmunoAssay System. (2772)

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Circ. Res. 95, 76–83. Mechanisms of cardiac nerve sprouting after myocardial infarction in dogs. 2003

Zhou, S., Chen, L.S., Miyauchi, Y., Miyauchi, M., Kar, S., Kangavari, S., Fishbein, M.C., Sharifi, B. and Chen, P.S.

Notes: After inducing myocardial infarction in feral dogs, transcardiac NGF levels were measured in the serum from 0.5 hours to 1 month later. This difference in NGF concentration between the coronary sinus and aorta was measured in triplicate samples using the NGF Emax® ImmunoAssay System. (3336)

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J. Neurochem. 83, 1129-1138. Extracellular ATP and nerve growth factor intensify hypoglycemia-induced cell death in primary neurons: role of P2 and NGFRp75 receptors. 2002

Cavaliere, F., Sancesario, G., Bernardi, G. and Volonte, C.

Notes: Cerebellar granule neurons were isolated from 8-day-old Wistar rats and were glucose deprived for 1 hour in Earl’s balanced salt solution. This solution was collected and assayed for NGF using the NGF Emax® ImmunoAssay System. (2809)

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Invest. Ophthalmol. Vis. Sci. 43, 987-994. The role of NGF signaling in human limbal epithelium expanded by amniotic membrane culture. 2002

Touhami, A., Grueterich, M. and Tseng, S.C.

Notes: Human amniotic membrane, either intact or epithelially denuded, was homogenized and NGF content measured using the NGF Emax® ImmunoAssay System. (2807)

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Brain Res. Mol. Brain Res. 87, 31-41. Axotomy alters neurotrophin and neurotrophin receptor mRNAs in the vagus nerve and nodose ganglion of the rat. 2001

Lee, P.G., Zhuo, H., and C.J. Helke

Notes: Protein and mRNA levels of various neurotrophins and neurotrophin receptors were analyzed in transected cervical vagus nerve and nodose ganglion. Nerve growth factor and neurotrophin-3 protein levels were measured using Promega's NGF Emax® ImmunoAssay System and NT-3 Emax® ImmunoAssay System, respectively. (2336)

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Clin. Can. Res. 7, 105-12. Nerve growth factor exerts differential effects on the growth of human pancreatic cancer cells. 2001

Zhu, Z.W., Friess, H., Wang, L., Bogardus, T., Korc, M., Kleeff, J., and Buchler M.W.

Notes: The levels of NGF in culture medium of numerous human pancreatic cancer cell lines was determined using Promega's NGF Emax® ImmunoAssay System. NGF-induced pancreatic cancer cell growth seemed to be dependent on the expression levels and the balance of the TrkA and p75 receptors. (2334)

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J. Neurosci. 20, 9096-9103. Co-Induction of p75NTR and p75NTR-Associated Death Executor in Neurons After Zinc Exposure in Cortical Culture or Transient Ischemia in the Rat 2000

Park, J.A., Lee, J.Y., Sato, T.A., and Koh, J.Y.

Notes: Mixed mouse cortical cultures containing both neurons and astrocytes were treated with zinc to induce neuronal death. Nerve growth factor augmented this zinc-induced neuronal death. The p75NTR receptor and the p75NTR-associated death executor (NADE) also play roles in zinc-triggered neuronal death. NGF levels were determined using Promega's NGF Emax® ImmunoAssay System. (2333)

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Endocrinology 141, 4736-4750. Direct effects of nerve growth factor on thecal cells from antral ovarian follicles. 2000

Dissen, G.A., Parrott, J.A., Skinner, M.K., Hill, D.F., Costa, M.E. and Ojeda, S.R.

Notes: Bovine thecal cells were grown for three days then subcultured for 24 hours. The culture media was then removed and NGF levels determined using the NGF Emax® ImmunoAssay System. (2810)

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J. Neuroimmunol. 106, 32-42. Interleukin 1-beta modulates the effects of hypoxia in neuronal culture. 2000

Di Loreto, S., Corvetti, L., Maccarone, R., Piancatelli, D., and Adorno D.

Notes: Levels of nerve growth factor in untreated and IL-1beta hippocampal neuronal cultures were monitored during homeostasis, hypoxia and reoxygenation. Untreated cells exhibited a decrease in both the expression and release of NGF under mild hypoxic stress whereas IL-1beta treated cells exhibited an increase in both expression and release of NGF. NGF levels were quantitated using Promega's NGF Emax® ImmunoAssay System. (2332)

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Endocrinology 141, 1073-82. Intraovarian excess of nerve growth factor increases androgen secretion and disrupts estrous cyclicity in the rat 2000

Dissen, G.A., Lara, H.E., Leyton, V., Paredes, A., Hill, D.F., Costa, M.E., Martinez-Serrano, A., and Ojeda, S.R.

Notes: A rat model of human polycystic ovarian syndrome in which NGF-producing neural progenitor cells were grafted into ovaries of juvenile rats was used to study the effect of NGF levels in ovary tissue. NGF levels in conditional culture medium from immortalized embryonic day 16 rat hippocampus-derived neural progenitor cells and in whole rat ovaries was determined using Promega's NGF Emax® ImmunoAssay System. (2331)

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Neuropharmacology 39, 1653-61. The role of cytokines and prostaglandin-E(2) in thymulin induced hyperalgesia. 2000

Safieh-Garabedian, B., Dardenne, M., Kanaan, S.A., Atweh, S.F., Jabbur, S.J., and Saade, N.E.

Notes: Thymulin induced thermal and mechanical hyperalgesia resulted in an increase in NGF, TNF alpha and PGE(2) levels in rat liver. NGF levels were determined with Promega's NGF Emax® ImmunoAssay System. (2335)

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Eur. J. Neurosci. 11, 2291-2305. Acute application of NGF increases the firing rate of aged rat basal forebrain neurons. 1999

Albeck, D.S., Backman, C., Veng, L., Friden, P., Rose, G.M. and Granholm, A.-C.E.

Notes: The NGF Emax® ImmunoAssay System (NGF ELISA) was used to quantitate NGF in the rat medial septium, vertical limb of the diagonal band, area CA1 of the hippocampus and dentate guyrus of the hippocampus. Some detail of the referenced extraction procedure is provided. (2222)

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Neuroscience 91, 1027-1034. Expression of nerve growth factor in astrocytes of the hippocampal CA1 area following transient forebrain ischemia. 1999

Gottlieb, M., and Matute, C.

Notes: The NGF EMAX® ImmunoAssay System (NGF ELISA) was used to assay for NGF protein levels in the CA1 region of the hippocampus from rats experiencing a transient ischemia and control animals. References and detail of the tissue preparation is provided. (1113)

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J. Neurosci. 18, 9326-9334. Dexamethasone induces hypertrophy of developing medial septum cholinergic neurons: Potential Role of nerve growth factor. 1998

Shi, B., Rabin, S.J., Brandoli, C., Mocchetti, I.

Notes: The NGF Emax® ImmunoAssay System was used to determine the level of NGF protein in different portions of the rat brain. Excellent detail is provided for sample preparation. The rats were treated for one week prior to assay with either vehicle or dexamethasone. NGF levels were normalized to the amount of protein in the extracts. (0390)

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Proc. Natl. Acad. Sci. USA 95, 7784-7788. Pancreatic beta cells synthesize and secrete nerve growth factor 1998

Rosenbaum, T., Vidaltamayo, R., Sanchez-Soto, M.C., Zentella, A., Hiriart, M.

Notes: The NGF Emax® ImmunoAssay System was used to measure NGF released into conditioned media of cultured rat pancreatic beta cells. The amount of NGF released increased with glucose treatment of the cells. The release was not inhibited by KCl but was inhibited by dibutryl cAMP. (0455)

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Eur. J. Immunol. 28, 3240-3251. Role of nerve growth factor in a mouse model of allergic airway inflammation and asthma. 1998

Braun, A., Appel, E., Baruch, R., Herz, U., Botchkarev, V., Paus, R., Brodie, C., Renz, H.

Notes: The NGF Emax® ImmunoAssay System was used to determine the NGF content of mouse lavage and serum as well as the conditioned media of mononuclear cells. Allergic conditions showed increases in NGF. (1400)

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