Wang, J., Ducret, A, Tu, Y., Kozasa, T., Aebersold, R., Rose, E.
Notes: The Sequencing Grade Modified Trypsin was used to digest proteins directly on nitrocellulose by referenced methods. Briefly, nitrocellulose bands were excised, cut in 1mm squares, and incubated for 18 hours at 37°C in 15-20µl of 15mM N-ethylmorpholine, 5mM acetic acid, 1% Zwittergent 3-16 that contained 1µg of Sequencing Grade Modified Trypsin. (0201)