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J. Biol. Chem. 272, 17112-17117. Structure of the m1 muscarinic acetylcholine receptor gene and its promoter 1997

Pepitoni, S., Wood, I.C. Buckley, N.J.

Notes: The Dual-Luciferase® Reporter Assay System was used to study transfections of IMR32 and NIH3T3 cells with firefly luciferase vector (pGL3 Basic) constructs. Transfections were controlled with co-transfected pRL-CMV Vector. The promoter under study was truncated with the Erase-A-Base® System. Tth DNA Polymerase was used as a high temperature reverse transcriptase. SP6 RNA Polymerase was used to produce probes for RNase protection assays. (0558)

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J. Biol. Chem. 272, 26285-26294. TGT3, thyroid transcription factor I, and Sp1 elements regulate transcriptional activity of the 1.3-kilobase pair promoter of t1alpha, a lung alveolar type I cell gene 1997

Ramirez, M.I., Rishi, A.K., Cao, Y.X., Williams, M.C.

Notes: Luciferase studies were performed in SV40 T type II cells and IMR90 fibroblasts using constructs prepared in the pGL3 Basic Vector. Luciferase activity was measured with the Luciferase Assay System. The Erase-A-Base® System was used to generate deletion mutants of the promoter. The hepatocyte nuclear factor 3beta was produced in vitro with the TNT® Coupled Reticulocyte Lysate System and used for gel shift analysis. (0495)

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J. Biol. Chem. 272, 30662-30671. The Human CC Chemokine Receptor 5 (CCR5) Gene. Multiple transcripts with 5'-end heterogeneity, dual promoter usage, and evidence for polymorphisms within the regulatory regions and noncoding exons 1997

Mummidi, S., Ahuja, S.S., McDaniel, B.L., Ahuja, S.K.

Notes: Reporter studies were performed in THP-1 monocytic cells, Jurkat T-cell leukemic cells and K562 myelogenous leukemia cells. Experimental promoter constructs were prepared in the pGL3 Basic Vector. Transfections were control by cotransfection with the pRL-CMV Vector, a source of Renilla luciferase. The activity of the two luciferases was determined with the Dual-Luciferase® Reporter Assay System. Ratios of pGL3-based Vectors to the pRL-CMV Vector were not reported but 0.5µg of the pRL-CMV Vector was used in each transfection. (0630)

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J. Biol. Chem. 272, 6979-6985. The rat arylalkylamine N-acetyltransferase gene promoter. cAMP activation via a cAMP-responsive element-CCAAT complex. 1997

Baler, R., Covington, S. and Klein, D.C.

Notes: Promega's Luciferase Assay System and pGL3 Basic Vector were used to study gene expression in primary rat pinealocytes. (1455)

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J. Biol. Chem. 272, 21090-21095. The rat glucocorticoid receptor mutant K461A differentiates between two different mechanisms of transrepression. 1997

Meyer, T., Starr, D.B., Carlstedt-Duke, J.

Notes: Luciferase studies were performed in HOS D4 osteosarcoma cells. Reporter constructs were prepared in the pGL2 Enhancer Vector. (0686)

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J. Biol. Chem. 272, 20522-20530. The rat pyruvate carboxylase gene structure. Alternate promoters generate multiple transcripts with the 5´-end heterogeneity. 1997

Jitrapakdee, S., Booker, G.W., Cassady, A.I., Wallace, J.C.

Notes: Luciferase reporter studies were performed in COS-1 cells using constructs prepared in the pGL3-Basic Vector. DNA sequencing was accomplished with the fmol® DNA Cycle Sequencing System. (0983)

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Proc. Natl. Acad. Sci. USA 94, 7400-7405. Tissue-specific knockout of the mouse Pig-a gene reveals important roles for GPI-anchored proteins in skin development. 1997

Tarutani, M., Itami, S., Okabe, M., Ikawa, M., Tezuka, T., Yoshikawa, K., Kinoshita, T., Takeda, J.

Notes: Luciferase studies were performed in PAM 212 mouse keratinocyte cells and NIH3T3 cells. They cloned a 14kb promoter fragment into the pGL3-Basic Vector and used this and the pGL3-Control Vector for their studies. (0303)

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J. Biol. Chem. 272, 25890-25898. Transcription of human ABO histo-blood group genes is dependent upon binding of transcription factor CBF/NF-Y to minisatellite sequence. 1997

Kominato, Y., Tsuchiya, T., Hata, N., Takizawa, H., Yamamoto, F.I.

Notes: Luciferase studies were performed in KATO III human gastric cancer cells using constructs prepared in the pGL3-Basic and pGL3-Promoter Vectors. (0879)

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J. Biol. Chem. 272, 23489-23497. Transcriptional regulation of the mouse presenilin-1 gene. 1997

Mitsuda, N., Roses, A.D. and Vitek, M.P.

Notes: The Dual-Luciferase™ Reporter System was used to quantitate the presenilin promoter activity in Neuro2a neuroblastoma cells, mouse P19 embryonal carcinoma cells and NIH 3T3 cells. Studies were also performed in P19 cells treated with retinoic acid to acquire a neuron-like phenotype and P19 cells treated with dimethyl sulfoxide to acquire a muscle-like phenotype. The presenilin promoter functioned best in the Neuro2a and neuron-like P19 cells. 5´-RACE products from mouse brain RNA were purified with the Wizard® PCR Preps System and cloned into the pGEM-T Vector . The cloned amplimers were sequenced and used as a template for amplification to produce truncation mutants to assess promoter activity. (1590)

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J. Biol. Chem. 272, 26360-26366. Transcriptional repression mediated by the PR domain zinc finger gene RIZ 1997

Xie, M. , Shao, G. , Buyse, I. M. , Huang, S.

Notes: Expression of luciferase reporter constructs, derived from the pGL3 Promoter Vector, was studied in 3T3 cells. (0165)

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J. Biol. Chem. 272, 30208-30214. Two naturally occurring insulin receptor tyrosine kinase domain mutants provide evidence that phosphoinositide 3-kinase activation alone is not sufficient for the mediation of insulin's metabolic and mitogenic effects. 1997

Krook, A., Whitehead, J.P., Dobson, S.P., Griffiths, M.R., Ouwens, M., Baker, C., Hayward, A.C., Sen, S.K., Maassen, J.A., Siddle, K., Tavaré, J.M., O'Rahilly, S.

Notes: CHO-K1 cells were transfected at ~80% confluency with four plasmids: (1) a CMV-driven vector expressing the wildtype or mutant human insulin receptor; (2) a pGL3-Basic-derived Vector containing five GAL4 binding sites upstream of the firefly luciferase reporter; (3) the pRL-CMV Control Vector; and (4) a fusion of the GAL4-binding domain and Elk-1 transcription factor. The Dual-Luciferase® Reporter Assay System was used to confirm that specific mutations in the insulin receptor negate its ability to activate the Elk-1 transcription factor. (0896)

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Hum. Gene Ther. 7, 1205-1217. An improved plasmid DNA expression vector for direct injection into skeletal muscle. 1996

Hartikka, J., Sawdey, M., Cornefert-Jensen, F., Margalith, M., Barnhart, K., Nolasco, M., Vahlsing, H.L., Meek, J., Marquet, M., Hobart, P., Norman, J., Manthorpe, M.

Notes: pGL3-Control Vector and the Luciferase Assay System were used for in vivo studies on murine quadricep muscles by injection of DNA. Extracts for assaying the tissues were prepared using the CCLR. (1066)

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Gene Ther. 3, 789-796. Effect of amniotic fluid on cationic lipid mediated transfection and retroviral infection. 1996

Douar, A. M. , Themis, M. , Sandig, V. , Friedmann, T. , Coutelle, C.

Notes: The Tfx™-50 Reagent was used to transfect pGL3 Vectors into NIH3T3 cells as well as microinjection into amniotic fluid (clone A31). (1243)

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