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Brain Res. 829, 18-27. Diverse stimuli induce calpain overexpression and apoptosis in C6 glioma cells. 1999

Ray, S.K., Wilford, G.G., Crosby, C.V., Hogan, E.L., Banik, N.L.

Notes: The Apoptosis Detection System, Fluorescein was used to identify apoptotic cells in C6 glioma cultures in response to various reagents. TUNEL Update: The Apoptosis Detection System, Fluorescein has been renamed to DeadEnd™ Fluorimetric TUNEL System. (0504)

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Br. J. Pharmacol. 127, 813-825. Induction of apoptosis in human mitogen-activated peripheral blood T-lymphocytes by the ether phospholipid ET-18-OCH3: Involvement of the Fas receptor/ligand system. 1999

Cabaner, C., Gajate, C., Macho, A., Munoz, E., Modolell, M., Mollinedo, F.

Notes: The Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL Assay) was used to assess apoptosis in mitogen-activated T cells treated with the ether phospholipid. The phospholipid did not induce apoptosis in resting T cells. (1376)

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Eur. J. Neurosci. 11, 769-780. Neuregulin is a mitogen and survival factor for olfactory bulb ensheathing cells and an isoform is produced by astrocytes. 1999

Pollock, G.S., Franceschini, I.A., Graham, G., Marchionni, M.A., Barnett, S.C.

Notes: The Apoptosis Detection System, Fluorescein was used to identify apoptotic primary olfactory bulb ensheathing cells. Cells were purified by FACS with a specific cell surface molecule, then plated onto poly-L-lysine coverslips. Good detail is provided for cell processing for the TUNEL assay. Update: The Apoptosis Detection System, Fluorescein has been renamed as DeadEnd™ Fluorometric TUNEL System (Cat.# G3250). (0539)

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J. Biol. Chem. 274, 30764-30769. Presenilin 1 protein directly interacts with Bcl-2. 1999

Alberici, A., Moratto, D., Benussi, L., Gasparini, L., Ghidoni, R., Gatta, L.B., Finazzi, D., Frisoni, G.B., Trabucchi, M., Growdon, J.H., Nitsch, R.M. and Binetti, G.

Notes: The Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL Assay) was used to monitor the apoptosis of H4 human neuroglioma cells following serum withdrawal and treatment with staurosporine. Over 40% of the cells were positive for TUNEL staining. (1504)

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J. Physiol. (Lond.) 515.2, 355-365.. Pulsatile shear stress leads to DNA fragmentation in human SH-Sy5Y neuroblastoma cell line. 1999

Triyoso, D.H., Good, T.A.

Notes: The Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL System) was used to label fragmented genomic DNA in the SH-Sy5Y neuroblastomas that had undergone shear stress. This TUNEL assay was used in conjunction with an LDH assay to confirm the mode of cell death. The TUNEL labeling was analyzed by flow cytometry. (0232)

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Biochem. J. 340, 33-40. Reactive oxygen species activate a Ca2+-dependent cell death pathway in the unicellular organism Trypanosoma brucei brucei 1999

Ridgley, E.L., Ziong, Z., Ruben, L.

Notes: Trypanosomes were treated with xanthine and xanthine oxidase to create reactive oxygen species. The cells were affixed to poly-L-lysine coated slides, fixed in 3% paraformaldehyde, and permeablized with 0.1% Triton® X-100 for TUNEL positive cells. TUNEL staining was provided by the Apoptosis Detection System, Fluorescein. Update: The Apoptosis Detection System, Fluorescein has been renamed DeadEnd™ Fluorometric TUNEL System. (0475)

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Am. J. Physiol. 277, H1593-H1599. Sustained JNK activation induces endothelial apoptosis: Studies with colchicine and shear stress. 1999

Hu, Y.-L., Li, S., Shyy, J.Y.-J., Chien, S.

Notes: The Apoptosis Detection System, Fluorescein, was used in triple fluorescent labeling. Bovine aortic endothelial cells were treated with colchicine and analyzed for the presence of β-Galactosidase (marker for transfection), TUNEL and nuclei (Hoechst dye). Transfection with a dominant negative mutant JNK (K-R) prevented the appearance of TUNEL staining in the cells and also eliminated the DNA ladder characteristic of apoptosis. The name of the Apoptosis Detection System, Fluorescein, has been changed to DeadEnd™ Fluorometric TUNEL System. (1025)

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Proc. Natl. Acad. Sci. USA 95, 9938-9943. A unique pattern of photoreceptor degeneration in cyclin D1 mutant mice. 1998

Ma, C., Papermaster, D., Cepko, C.L.

Notes: The Apoptosis Detection System, Fluorescein, was used to examine apoptosis in the retinas of cyclin D1 deficient transgenic mice. The name of the Apoptosis Detection System, Fluorescein, has been changed to DeadEnd™ Fluorometric TUNEL System. (0757)

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Oncogene 16, 1533-1542. Herbimycin A accelerates the induction of apoptosis following etoposide treatment or γ-irradiation of bcr/abl-positive leukaemia cells 1998

Riordan, F.A., Bravery, C.A., Mengubas, K., Ray, N., Borthwick, N.J., Akbar, A.N., Hart, S.M., Hoffbrand, A.V., Mehta, A.B., Wickremasinghe, R.G.

Notes: The Apoptosis Detection System, Fluorescein was used to detect apoptosis in HL60 and K562 cells treated with etopiside, herbimycin A and a etopiside/herbimycin A mixture. The etopiside/herbimycin A mixture induced the most dramatic level of apoptosis on K562 as judged by the TUNEL assay and a cell morphology assay. The etopiside treatment of HL60 cells produced the most significant amount of apoptosis in this cell type. The degree of apoptosis in HL60 cells was dramatically reduced in the presence of herbimycin A. Herbimycin A treatment coupled with irradiation produced dramatic apoptosis of K562 cells. The same tests were also performed with Tom 1 and KCL-22 cells. Update: The Apoptosis Detection System, Fluorescein has been renamed to DeadEnd™ Fluorimetric TUNEL System. (0478)

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J. Neurosci. 18, 923-931. Nitric oxide and superoxide contribute to motor neuron apoptosis induced by trophic factor deprivation. 1998

Estevez, A. G. , Spear, N. , Manuel, S. M. , Radi, R. , Henderson, C. E. , Barbeito, L. , Beckman, J. S.

Notes: The Apoptosis Detection System, Fluorescein was used to monitor apoptosis in motor neurons deprived of BDNF. Motor neurons were derived from day 15 rat embryos. Contains a figure of a apoptotic neuron stained with the fluoresceinated dUTP and propidium iodide. (1179)

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Proc. Natl. Acad. Sci. USA 95, 993-998. The von Hippel-Lindau tumor suppressor gene is required for cell cycle exit upon serum withdrawal 1998

Pause, A., Lee, S., Lonergan, K.M., Klausner, R.D.

Notes: Promega's Apoptosis Detection System, Fluorescein was used to confirm that the 786-0 human sporadic renal carcinoma cells were apoptotic following serum withdrawal. Staining with the TUNEL assay was used as a confirmation of the visual identification of pycnotic nuclei. The data is not shown in the paper.  Update: The Apoptosis Detection System, Fluorescein has been renamed to DeadEnd™ Fluorometric TUNEL System (Cat.# G3250). (0553)

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EMBO J. 16, 5639-5653. Cooperation of Spi-1/PU.1 with an activated erythropoietin receptor inhibits apoptosis and Epo-dependent differentiation in primary erythroblasts and induces their Kit ligand-dependent proliferation 1997

Quang, C.T., Wessely, O., Pironin, M., Beug, H., Ghysdael, J.

Notes: The Apoptosis Detection System, Fluorescein was used for TUNEL assays on erythroblast clones. Cells were also stained with propidium iodide. The labeled cells were analyzed by flow cytometry to determine the percentage of apoptotic cells and the cell cycle distribution. TGFα (discontinued) was used to maintain erythroid progenitors in culture. Update: The Apoptosis Detection System, Fluorescein has been renamed to DeadEnd™ Fluorimetric TUNEL System. (0526)

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J. Clin. Invest. 99, 1897-1905. Molecular basis of autosomal dominant neurohyposphyseal diabetes insipidus: Cellular toxicity caused by the accumulation of mutant vasopressin precursors within the endoplasmic reticulum. 1997

Ito, M., Jameson, J.L., Ito, M.

Notes: Mutant and wildtype arginine vasopressin (AVP) constructs were co-transfected with a CAT-expression vector containing a neo resistance gene. Stable transformants of Neuro2A cells were generated with the aid of the Transfectam® Reagent and G418 sulfate. CAT activity in stably transfected clones was determined with the CAT Enzyme Assay System. The effect of the mutant AVP constructs on cell viability was determined with the CellTiter 96® AQueous Cell Proliferation Assay (MTS/PMS). Several constructs displayed marked decreases in cell viability. The cause was necrosis rather than apoptosis due to the absence of DNA laddering and a negative reaction in the Apoptosis Detection System, Fluoroscein (data not shown). The name of the Apoptosis Detection System, Fluorescein, has changed to DeadEnd™ Fluorometric TUNEL System. (1009)

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