Wang, T.G., Gotoh, Y., Jennings, M.H., Rhoads, C.A. and Aw, T.Y.
Notes: The Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL System) was used to assess apoptotic response of a human colonic cell line (CaCo-2) to lipid hydroperoxide levels. Dose dependence between 0 and 25µM was studied for 24hrs, and a time course using 10µM was done from 0-48 hrs. Cells were washed with PBS, fixed in 1% formaldehyde, and permeabilized with 70% ice cold ethanol at -20 degrees overnight. The permeabilized cells were washed, labeled with TdT and fluorescein, and incubated with 5µg/ml PI. Labeled cells were quantified by flow cytometry. (2126)