13, 2325 - 2332.
Adenoviral vectors expressing siRNAs for discovery and validation of gene function.
Arts, G.A., Langemeijer, E., Tissingh, R., Ma, L., Pavliska, H., Dokic, K., Dooijes, R., Mesic, E., Clasen, R., Michiels, F., van der Schueren, J., Lambrecht, M., Herman, S., Brys, R., Thys, K., Hoffmann, M., Tomme, P. and van Es, H.
Notes: The SV 96 Total RNA Isolation System was used to isolate total RNA from human umbilical vein endothelial (HUVEC) cells, primary keratinocytes (NHEK-adults) and human rheumatoid arthritis synovial fibroblasts (RASF) infected with adenovirus expressing siRNAs targeted at a variety of messages. The isolated RNA was used in real-time SYBR Green RT-PCR reactions to investigate the amount of message present after infection. For reverse transcriptase reactions, the researchers used 5-100ng of purified total RNA. Results were normalized to GAPDH message levels. (2752)
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