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Antimicrob. Agents Chemother. 42, 2284-2289. Two 2-hydroxy-3-alkyl-1,4-naphthoquinones with in vitro and in vivo activities against Toxoplasma gondii. 1998

Khan, A.A., Nasr, M., Araujo, F.G.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) was used to monitor the toxic effects of uninfected human foreskin fibroblasts after exposure to the naphthoquinones being tested for anti-toxoplasma activities. (0933)

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J. Clin. Invest. 99, 2284-2292. A peptidomimetic antagonist of the αvβ3 integrin inhibits bone resorption in vitro and prevents osteoporosis in vivo 1997

Engleman,V.W., Nickols, G.A., Ross, F.P., Horton, M.A., Griggs, D.W., Settle, S.L., Ruminski, P.G., Titelbaum, S.L.

Notes: The affect of the RGD peptidomimetic SC56631 on the ability of human K562 erythroleukemia cells and human embryonic kidney 293 cells to adhere to fibronectin was assessed. Cells were preincubated with the test compound and then plated in 96-well plates coated with fibronectin. Plates were washed, and attached cells were quantitated using the CellTiter 96® Non-Radioactive Cell Proliferation Assay. (2518)

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Proc. Natl. Acad. Sci. USA 94, 8982-8987. Activities and response to DNA damage of latent and active sequence-specific DNA binding forms of mouse p53 1997

Wu, Y. , Huang, H. , Miner, Z. , Kulesz-Martin, M.

Notes: The inhibition of proliferation of Saos-2 human osteosarcoma cells was monitored with the CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT). (0161)

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Proc. Natl. Acad. Sci. USA 94(17), 8982-8987. Activities and response to DNA damage of latent and active sequence-specific DNA binding forms of mouse p53. 1997

Wu, Y., Huang, H., Miner, Z., and Kulesz-Martin, M.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT version) was used to determine relative cell number in growth inhibition assays using human osteosarcoma cells and murine embryonic fibroblasts lacking endogenous p53 expression. Saos2 cells and (10)1 fibroblast clones were transfected to contain inducible p53as or p53 and demonstrated to be growth inhibited upon expression of those genes. (1727)

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Blood 89(7), 2529-2536. Adoptive transfer of anti-CD3-activated CD4+ T cells plus cyclophosphamide and liposome-encapsulated Interleukin-2 cure murine MC-38 and 3LL tumors and establish tumor-specific immunity. 1997

Saxton, M.L., Longo, D.L., Wetzel, H.E., Tribble, H., Alvord, W.G., Kwak, L.W., Leonard, A.S., Ullmann, C.D., Curti, B.D.and Ochoa, A.C.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to measure proliferation of murine MC-38 colon adenocarcinoma cells treated with supernatants from CD4+ T cells activated with anti-CD3 and IL-2. (1705)

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J. Biol. Chem. 272., 27913-27918.. Interaction between the adhesion receptor, CD44, and the oncogene product, p185HER2, promotes human ovarian tumor cell activation 1997

Bourguignon, L. Y. , Zhu, H. , Chu, A. , Iida, N. , Zhang, L. , Hung, M. C.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay was was used to monitor the in vitro proliferation of SKOV3.ipl human ovarian tumor cells. (1391)

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J. Biol. Chem. 272(44), 27913-27918. Interaction between the adhesion receptor, CD44, and the oncogene product, p185HER2, promotes human ovarian tumor cell activation. 1997

Bourguignon, L.Y.W., Zhu, H., Chu, A., Iida, N., Zhang, L. and Hung, M.C.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to determine if hyaluronic acid (HA) stimulates growth of SKOV3.ipl human ovarian carcinoma cells. (1496)

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Cancer Res. 57, 4242-4248. Oxythiamine and dehydroepiandrosterone inhibit the nonoxidative synthesis of ribose and tumor cell proliferation. 1997

Boros, L.G., Puigjaner, J., Cascante, M., Lee, W-N.P., Brandes, J.L., Bassilian, S., Yusuf, F.I., Williams, R.D., Muscarella, P., Melvin, W.S. and Schirmer, W.J.

Notes: The MTT-based CellTiter® 96 Non-Radioactive Cell Proliferation Assay was used to investigate cell density and cell viability in response to different concentrations of dehydroepiandrosterone, xythiamine, or combinations on MIA PaCa-2 pancreatic carcinoma cells. (1495)

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Proc. Natl. Acad. Sci. USA 94, 14701-14706. Regulated expression of the diphtheria toxin A chain by a tumor- specific chimeric transcription factor results in selective toxicity for alveolar rhabdomyosarcoma cells. 1997

Massuda, E.S., Dunphy, E.J., Redman, R.A., Schreiber, J.J., Nauta, L.E., Barr, F.G., Maxwell, I.H., Cripe, T.P.

Notes: The Access RT-PCR System was used to demonstrate expression of the transfected cDNAs in cells. The CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) was used to monitor cell survival after diphtheria toxin exposure. (0698)

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Blood 89, 842-852. Roles of the N and C terminal domains of the interleukin-3 receptor alpha chain in receptor function. 1997

Barry, S.C., Korpelainen, E., Sun, Q., Stomski, F.C., Moretti, P.A., Wakao, H., D'Andrea, R.J., Vadas, M.A., Lopez, A.F. and Goodall, G.J.

Notes: The Altered Sites® II in vitro Mutagenesis System was used to generate a 237 base deletion in the N-terminal portion of the interleukin-3 receptor alpha chain, and to insert a premature stop codon to produce a C-terminal truncation. Mutations were created using the single stranded DNA protocol. Mutants were expressed in FDC-P1 cells and assayed for proliferation in response to rhIL-3 using the CellTiter 96®  Non-Radioactive Cell Proliferation Assay (MTT). (1463)

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J. Biol. Chem. 272, 13019-13025. TrkB variants with deletions in the leucine-rich motifs of the extracellular domain. 1997

Ninkina, N. , Grashchuck, M. , Buchman, V. L. , Davies, A. M.

Notes: The PolyATtract® System 1000 was used to isolate polyA+ RNA directly from newborn mouse brains. The RNA was used in RT-PCR for detection of specific TrkB variants. Taq DNA Polymerase and the pGEM®-T Vector System were used to produce and subclone novel TrkB variants that differ in the extracellular domain. The CellTiter 96® Non-Radioactive Cell Proliferation Assay was performed on NIH 3T3 cells transfected with the TrkB receptors and challenged with BDNF, NT3 and NT4/5. (0615)

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J. Biol. Chem. 271(37), 22441-22446. 4-Hydroxyphenyl retinamide is a highly selective activator of retinoid receptors. 1996

Fanjul, A.N., Delia, D., Pierotti, M.A., Rideout, D., Yu, J.Q., Pfahl, M., Qiu, J.

Notes: The CellTiter 96® System was used to determine the antiproliferative activity of 4HPR on various cell lines including T47D, MCF-7, Hs578T, Calu-6, H661, HeLa, and CV-1. (2880)

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J. Cell Biol. 135(4), 1085-1096. Adenovirus-mediated gene transfer of the tumor suppressor, p53, induces apoptosis in postmitotic neurons. 1996

Slack, R.S., Belliveau, D.J., Rosenberg, M., Atwal, J., Lochmuller, H., Aloyz, R., Haghighi, A., Lach, B., Seth, P., Cooper, E., Miller, F.D.

Notes: The MTT-based CellTiter 96® System was used to measure survival of rat primary sympathetic neurons in response to infection with varying titers of adenovirus or HSV-1. TUNEL staining using Promega's TdT also is shown. (2950)

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J. Biochem. Biophys. Methods 31, 123-134. Application of a chromogenic bioassay procedure for the measurement of the proliferation of endothelial cells in vitro under the influence of the effects of steroid hormones and growth factors. 1996

Drummond, A.E., McPherson, S.J., Laslett, A. and Hearn, M.T.W.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to measure the effects of combinations of βFGF and steroids (estradiol or progesterone) on proliferation of Balb/3T3 fibroblasts and bovine aortic endothelial cells (1502)

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Biol. Reprod. 55(6), 1333-1342. Growth factor control of cultured rat uterine stromal cell proliferation is progesterone dependent. 1996

Piva, M., Flieger, O. and Rider, V.

Notes: The MTT-based CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to measure proliferation in primary cultures of rat uterine stromal cells stimulated by combinations of steroid hormones and bFGF in serum-free medium. (2082)

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Biochem. Biophys. Res. Commun. 226(3), 935-941. Growth hormone-responsive DT-diaphorase-mediated bioreduction of tetrazolium salts. 1996

Goodwin, C.J., Holt, S.J., Riley, P.A., Downes, S. and Marshall, N.J.

Notes: The authors compared MTT (CellTiter 96® Non-Radioactive Cell Proliferation Assay) and MTS (CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay) tetrazolium salts and report that DT-diaphorase inhibitors abolish MTS but not MTT formazan production. The authors conclude that MTT and MTS/menadione resulted in formazan production via a different electron transfer pathway. (2117)

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Cell Biochem. Funct. 14(2), 121-129. Long-term treatment of Swiss 3T3 fibroblasts with dexamethasone attenuates MAP kinase activation induced by insulin-like growth factor-I (IGF-I). 1996

Hansson, A., Hehenberger, K. and Thoren, M.

Notes: A CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to determine that dexamethasone treatment did not affect IGF-I induced proliferation of Swiss 3T3 cells. The authors report the use of MTS; however, they describe the use of the Solubilization Solution and recording absorbance at 570nm which implies they were using the MTT-based assay. (2121)

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Cancer Res. 56(7), 1571-1577. Potential role for retinoic acid receptor-g in the inhibition of breast cancer cells by selective etinoids and interferons. 1996

Fanjul, A.N., Bouterfa, H., Dawson, M. and Pfahl, M.

Notes: The MTT-based CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to measure interferon-enhanced antiproliferative activity of retinoids on MCF-7 breast cancer cells. (2113)

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J. Cell Biol. 133(1), 211-220. UV activates growth factor receptors via reactive oxygen intermediates. 1996

Huang, R-P., Wu, J-X., Fan, Y. and Adamson E.D.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to measure growth of NIH 3T3 and the HC11 clone of the mouse mammary COMMA-1D cells. Cells exposed to 20 J/m2 UVC in the presence of 50µM suramin were growth inhibited compared to controls without suramin treatment. (2087)

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Growth Reg. 5(2), 69-84. A critical assessment of the use of microculture tetrazolium assays to measure cell growth and function. 1995

Marshall, N.J., Goodwin, C.J. and Holt, S.J.

Notes: A good general review of the use of tetrazolium compounds including MTS for microculture tetrazolium assays. (2071)

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In Vitro Cell. Dev. Biol. Anim. 31(4), 295-299. Alteration of epithelial cell lipid synthesis by N-nitrosonornicotine. 1995

Schuster, G.S., Caughman, G.B., and Dirksen, T.R.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to measure growth effects of N-nitrosonornicotine on hamster oral epithelial cells cultured in keratinocyte serum-free medium. (1708)

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Development 121, 2681-2693. Analysis of function and expression of the chick GPA receptor (GPAR α) suggests multiple roles in neuronal development. 1995

Heller, S., Finn, T.P., Huber, J., Nishi, R., Geissen, M., Puschel, A.W., Rohrer, H.

Notes: The authors used the CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) and Anti-β-Galactosidase mAb in a TF-1 cell line. (1034)

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Methods Cell Biol. 46, 187-216. Calcium, free radials, and excitotoxic neuronal death in primary cell culture. 1995

Mattson, M.P., Barger, S.W., Begley, J.G. and Mark, R.J.

Notes: In this paper, the CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to assess cell viability. (1518)

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J. Immunol. 154(9), 4741-4748. Chloromethyl ketones block induction of nitric oxide synthase in murine macrophages by preventing activation of nuclear factor-KappaB. 1995

Kim, H., Lee, H.S., Chang, K.T., Ko, T.H., Baek, K.J. and Kwon, S.K.

Notes: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS) was used to determine viability of TPCK and TLCK treated murine macrophages. (2099)

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J. Clin. Endocrinol. Metab. 80(4), 1431-1437. Dysregulation of interleukin-6 response in extopic endometrial stromal cells: correlation with decreased soluble receptor levels in peritoneal fluid of women with endometriosis. 1995

Rier, S.E., Zarmakoupis, P.N., Hu, X. and Becker, J.L.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) was used to measure human endometrial cell proliferation. (2085)

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