We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation
Home » Resources » Tools »

Citations Search

Catalog_banner_2014

Need Assistance? Chat

Sort By:

J. Biol. Chem. 273, 22877-22883. Insulin-like growth factor-I augments erythropoietin-enduced proliferation through enhanced tyrosine phosphorylation of STAT5. 1998

Okajima, Y., Matsumura, I., Nishiura, T., Hashimoto, K., Yoshida, H., Ishikawa, J., Wakao, H., Yoshimura, A., Kanakura, Y., Tomijama, Y., Matsuzawa, Y.

Notes: Studies were performed in F-36P, a human IL-3 dependent erythroleukemia cell line. Cells were electroporated with either an AP-1 specific luciferase reporter vector or a STAT5-specific luciferase reporter vector. To normalize for electroporation efficiency, an equal amount of the pRL-CMV Vector was co-transfected with the luciferase reporters. Luciferase activities were monitored with the Dual-Luciferase® Reporter Assay System. (0599)

Expand Full Notes »

J. Biol. Chem. 273, 13552-13562. Isoforms of hepatocyte nuclear factor-6 differ in DNA-binding properties, contain a bifunctional homeodomain and define the new ONECUT class of homeodomain proteins. 1998

Lannoy, V.J., Bürglin, T.R., Rousseau, G.G., Lemaigre, F.P.

Notes: Studies were performed in HepG2 cells. Cells were transfected with a hepatocyte nuclear factor-6 (HNF-6)–responsive promoter firefly luciferase construct (3µg), various forms of the HNF-6 protein in an expression vector (400ng) and a Renilla luciferase control vector (pRL-null Vector) driven by the liver pfk-2 promoter (not responsive to HNF-6; 500ng). The ability of the various HNF-6 isoforms to drive luciferase expression was normalized to Renilla luciferase activity, and the luciferase activity was measured with the Dual-Luciferase® Reporter Assay System. The various HNF-6 constructs were also expressed in vitro with the TNT® Coupled Wheat Germ Extract System and used for gel shift assays. (0839)

Expand Full Notes »

J. Biol. Chem. 273, 8278-8286. Lymphoid-specific expression of the Id3 gene in hematopoietic cells. Selective antagonism of E2A basic helix-loop-helix protein associated with Id3-induced differentiation of erythroleukemia cells. 1998

Deed, R. W. , Jasiok, M. , Norton, J. D.

Notes: Promega's Taq DNA polymerase was used in construction of various plasmids. The authors used a mammalian two-hybrid system with a firefly luciferase reporter and pRL-CMV Vector as a transfection control. Luciferase activity was measured using the Dual-Luciferase® Reporter Assay System. (1266)

Expand Full Notes »

Circ. Res. 83, 508-515. Lysophosphatidylcholine enhances cytokine-induced interferon gamma expression in human T lymphocytes. 1998

Nishi, E., Kume, N., Ueno, Y., Ochi, H., Moriwaki, H., Kita, T.

Notes: Promoter studies were performed in human peripheral T lymphocytes. Experimental constructs in the pGL2-Basic Vector and cotransfected with the pRL-TK Vector at a 20:1 ratio. The luciferases were detected with the Dual-Luciferase® Reporter Assay System. (0616)

Expand Full Notes »

J. Biol. Chem. 273, 19130-19139. Phorbol ester-induced transcription of a fibroblast growth factor-binding protein is modulated by a complex interplay of positive and negative regulatory promoter elements. 1998

Harris, V.K., Liaudet-Coopman, E.D.E., Boyle, B.J., Wellstein, A., Riegel, A.T.

Notes: Reporter studies were performed in the ME-180 squamous cell carcinoma cell line. One microgram of the firefly luciferase construct was contransfected with 1ng of pRL-CMV Vector (1,000:1 ratio of firefly reporter to Renilla control), and luciferase activities were measured with the Dual-Luciferase® Reporter Assay System. The Kanamycin Control RNA was used as a control template for primer extension analysis. (1065)

Expand Full Notes »

Nature 391, 82-86. PPAR-γ agonists inhibit production of monocyte inflammatory cytokines. 1998

Jiang, C., Ting, A.T., Seed, B.

Notes: The authors used the Dual-Luciferase® Reporter System with U937 cells and the pRL-TK Vector. (0977)

Expand Full Notes »

Proc. Natl. Acad. Sci. USA 95, 8170-8174. Racial variability in the UDP-glucuronosyltransferase 1 (UGT1A1) promoter: A balanced polymorphism for regulation of bilirubin metabolism? 1998

Beutler, E., Gelbart, T. and Demina, A.

Notes: Studies were performed in HepG2 and HuH7 cells (both human cells of hepatic origin). One microgram of the experimental constructs in the pGL3 Basic Vector was cotransfected with 50ng of the pRL-SV Vector (20:1 ratio). Luciferase activity was measured using the Dual-Luciferase® Reporter Assay System. (1443)

Expand Full Notes »

J. Biol. Chem. 273, 27474-27483. Sp1 and Sp3 regulate transcriptional activity of the facilitative glucose transporter isoform-3 gene in mammalian neuroblasts and trophoblasts. 1998

Rajakumar, R.A., Thamotharan, S., Menon, R.K., Devaskar, S.U.

Notes: AMV Reverse Transcriptase was used for primer extension analysis from poly(A)+ RNA. The Riboprobe® in vitro Transcription System was used to generate [32P]-antisense RNA probes for RNase protection assays. Promoter studies were performed in N2A murine neuroblasotoma cells. Experimental constructs were assembled in the pGL2-Basic Vector and cotransfected with the pRL-TK Vector at a 10:1 ratio and luciferase activities determined with the Dual-Luciferase® Reporter Assay System. Some promoter studies were performed in Drosophila Schneider cells with luciferase promoter constructs and an RSV-driven beta-galactosidase vector. Beta-Galactosidase activity was determined with the Beta-Galactosidase Enzyme Assay System. (0531)

Expand Full Notes »

J. Biol. Chem. 273, 22075-22082. The cyclin E promoter is activated by human cytomegalovirus 86-kDa immediate early protein. 1998

Bresnahan, W.A., Albrecht T., Thompson, E.A.

Notes: Human diploid embryonic lung fibroblasts (LU) were transiently transfected using Tfx™-50 Reagent. The cells were 70-80% confluent, and the lipid to DNA ratio was 3:1 (transfection time = 2 hours; no serum). Activation of the cyclin promoter was measured using the Dual-Luciferase® Reporter Assay System. The Core Footprinting System was used to determine the promoter region that is bound by the cytomegalovirus immediate early protein. (1403)

Expand Full Notes »

J. Biol. Chem. 273, 26923-26930. The early growth response protein (EGR-1) regulates interleuking-2 transcription by synergistic interaction with the nuclear factor of activated T cells. 1998

Decker, E.L., Skerka, C., Zipfel, P.F.

Notes: The 293 cell line was cotransfected with a luciferase reporter containing the IL-2 promoter, an expression vector for either EGR-1, NFATc or both and finally the pRL-TK Vector. Reporter to control ratio was 4:1 and the luciferase activity was determined with the Dual-Luciferase® Reporter Assay System. (1265)

Expand Full Notes »

Genetics 149, 1125-1138. The maize regulatory gene B-Peru contains a DNA rearrangement that specifies tissue-specific expression through both positive and negative promoter elements. 1998

Selinger, D.A., Lisch, D., Chandler, V.L.

Notes: A plasmid, p35S-Rluc, consisting of the CaMV 35S promoter and ADH1-Intron1 fused to the Renilla luciferase gene as a transformation control in maize. The Dual-Luciferase® Reporter Assay System was used to detect and quantitate expression levels. (0422)

Expand Full Notes »

J. Biol. Chem. 273, 34775. The roles of nuclear factor of activated T cells and Ying-Yang 1 in activation-induced expression of the interferon-gamma promoter in T cells. 1998

Sweetser, M.T., Hoey, T, Sun, Y.L., Weaver, W.M., Price, G.A., Wilson, C.B.

Notes: Reporter assays were performed in primary murine splenocytes. The interferon-gamma promoter constructs were assembled in the pGL3-Basic Vector and a Renilla luciferase control vector was constructed with the pRL-null Vector and a β-actin promoter. The plasmids were electroporated at a 10:1 ratio. Luciferase activities were followed with the Dual-Luciferase® Reporter Assay System. (0285)

Expand Full Notes »

J. Lipid Res. 39, 1520-1524.. The –514 polymorphism in the hepatic lipase gene (LIPC) does not influence androgen-mediated stimulation of hepatic lipase activity. 1998

Vega, G.L., Gao, J., Bersot, T.P., Mahley, R.W., Verstraete, R., Grundy, S.M., White, A., Cohen, J.C.

Notes: Studies were performed in HepG2 cells. Experimental promoter contructs were assembled in the pGL3-Basic Vector and transfection efficiency was monitored by cotransfecting the pRL-CMV Vector. Luciferase activities were assayed with the Dual-Luciferase® Reporter Assay System. The ratio of experimental:control vector were not reported. (0219)

Expand Full Notes »

Biochem. J. 332, 773-780. Transcript heterogeneity of the human reduced folate carrier results from the use of multiple promoters and variable splicing of alternative upstream exons. 1998

Zhang, L. , Wong, S. C. , Matherly, L. H.

Notes: The authors used the PolyATtract® mRNA Isolation System to isolate mRNA from total RNA. They used this mRNA-rich fraction for primer extension analysis using Promega's AMV Reverse Transcriptase. The Wizard® Plus Midiprep DNA Purification System was used for various plasmid isolations and the Erase-a-Base®  System was used to generate deletion series. The Dual-Luciferase® Reporter Assay System was used to study promoters cloned into the pGL3-Basic Vector. The pRL-SV40 was used as a transfection control plasmid. (0095)

Expand Full Notes »

J. Biol. Chem. 273, 21137-21144. Transcriptional activation of the p21WAF1, CIP1, SDI1 gene by interleukin-6 type cytokines: A prerequisite for their pro-differentiating and anti-apoptotic effects on human osteoblastic cells. 1998

Bellido, T., O'Brien, C.A., Roberson, P.K. and Manolagas, S.C.

Notes: Studies were performed in MG63 human osteosarcoma cells. Firefly luciferase activity was normalized to Renilla luciferase activity using the Dual-Luciferase® Reporter Assay System. The cells were transfected with the pGL2-Basic Vector containing the experimental promoters, pRL-CMV Vector, and a Stat-3 expression vector. The ratio of pGL2 Vector to pRL-CMV Vector was 1000:1. The ratio of pGL2 Vector to Stat3 Expression Vector was 6:1. The chemical MTS was used in a LDH-coupled assay to determine cell viability of osteosarcoma cells transfected with antisense oligonucleotides. (1436)

Expand Full Notes »

J. Biol. Chem. 273, 11923-11929. Transcriptional regulation of the prothrombin gene in muscle. 1998

Kim, S., Nelson, P.G.

Notes: Studies were performed in HepG2 cells and mouse C2 skeletal muscle cells. The experimental pGL3-Basic Vector-derived constructs were cotransfected with pRL-CMV Vector using the calcium phosphate method. Luciferase activity was monitored with the Dual-Luciferase® Reporter Assay System 48 hours after transfection. (0899)

Expand Full Notes »

Mol. Cell. Biol. 18, 6178-6190. Two independent internal ribosome entry sites are involved in translation initiation of vascular endothelial growth factor mRNA. 1998

Huez, I., Creancier, L., Audigier, S., Gensac, M.C., Prats, A.C., Prats, H.

Notes: The authors used the Dual-Luciferase® Reporter Assay System to study IRES sequences. They placed the Renilla gene upstream of the firefly gene with sequences containing and IRES sequence between them. Then they performed deletional studies on this region to identify minimal necessary sequences for IRES function and allowed for both firefly and Renilla luciferase expression. (1032)

Expand Full Notes »

J. Biol. Chem. 273, 29816-29821. Two YY-1-binding proximal elements regulate the promoter strength of the TATA-less mouse ribonucleotide reductase R1 gene. 1998

Johansson, E., Hjortsberg, K., Thelander, L.

Notes: The authors cloned promoter region (and deletion mutants) of mouse ribonucleotide reductase R1 gene into pGL3-Basic Vector. Also, they cloned 3´-UTR of this gene downstream of firefly luciferase coding region in pGL3-Control Vector to study posttranscriptional effects mediated by this region. Co-transfected plasmids with pRL-SV40 into Balb/3T3 fibroblasts. Assayed luciferase activity with Dual-Luciferase® Reporter Assay System. (0985)

Expand Full Notes »

J. Biol. Chem. 272, 31793-31800. CCAAT/Enhancer-binding protein delta activates insulin-like growth factor-I gene transcription in osteoblasts. Identification Of a novel cyclic amp signaling pathway in bone 1997

Umayahara, Y., Ji, C., Centrella, M., Rotwein, P., McCarthy, T.L.

Notes: Studies were performed in COS-7 cells. The experimental firefly luciferase vector (derived from pGL2-Basic Vector) was co-transfected with the Renilla control Vector (pRL-CMV Vector) and plasmids expressing either the CCAAT/Enhancer-binding protein beta or delta from a CMV promoter. The firefly luciferase:expression plasmid ratio was 10:1 and the firefly luciferase:Renilla luciferase vector ratio was 1000:1. In vitro translations were performed with the TNT® Coupled Reticulocyte Lysate System. (0252)

Expand Full Notes »

J. Biol. Chem. 272, 31278-31284. Differential regulation of the transcriptional activity of the orphan nuclear receptor NGFI-B by membrane depolarization and nerve growth factor. 1997

Katagiri, Y., Hirata, Y., Milbrandt, J., Guroff, G.

Notes: Dual-Luciferase® Reporter Assay System was used to perform studies in rat PC12 cells. The firefly luciferase reporter vector was transfected with the control plasmid (pRL-TK Vector) at a 20:1 ratio. Eighteen hours after transfection, the cells were treated with NGF, EGF or KCl and assayed up to eight hours later. In some experiments, PC12 cells were co-transfected with the firefly luciferase plasmid, the Renilla control vector and a vector expressing the wildtype or mutant NGFI-B protein. (0920)

Expand Full Notes »

J. Biol. Chem. 272, 23659-23667. Differential transcriptional regulation of the two vascular endothelial growth factor receptor genes. 1997

Gerber, H-P., Condorelli F., Park J. and Ferrara N.

Notes: Promega's pGL2 Vectors; Dual-Luciferase® Reporter Assay System and Access RT-PCR System were used in this study. (1992)

Expand Full Notes »

Proc. Natl. Acad. Sci. USA 94, 12309-12313. Expression of hepatocyte nuclear factor 6 in rat liver is sex-dependent and regulated by growth hormone. 1997

Lahuna, O., Fernandez, L., Karlsson, H., Maiter, D., Lemaigre, F.P., Rousseau, G.G., Gustafsson, J., Mode, A.

Notes: Dual-Luciferase® Reporter Assay System studies were performed in HepG2 cells. The experimental plasmid constructed in pGL2-Basic Vector was used at a 40:1 ratio over the pRL-CMV Vector. The experimental and control plasmids were cotransfected with a third plasmid expressing hepatocyte nuclear factor 6 from a CMV promoter. The luciferase activity expressed in the presence or absence of the hepatocyte factor were measured. The TNT® Coupled Wheat Germ Extract System was used to in vitro translate four transcription factors for assay of gel shift with the promoter construct. (0870)

Expand Full Notes »

J. Biol. Chem. 272, 30583-30588. Identification of a Placental Enhancer for the Human Leptin Gene 1997

Bi, S., Gavrilova, O., Gong, D.W., Mason, M.M. and Reitman, M.

Notes: Promoter activity was assayed in JEG-3 choriocarcinoma cells, JAR choriocarcinoma cells, HeLa cells and primary adipocytes. The pGL3 Vectors were transfected at a 20:1 ratio over pRL-CMV Vector. Enhancer activity was functional only in the choriocarcinoma cells. The Dual-Luciferase® Reporter Assay System was used to measure luciferase expression. (1446)

Expand Full Notes »

Virology 238, 432-443. Increasing the ratio of PP2A core enzyme to holoenzyme inhibits Tat-stimulated HIV-1 transcription and virus production. 1997

Ruediger, R., Brewis, N., Ohst, K. and Walter, G.

Notes: This paper describes a method for quantitation of luciferase mRNA by in vitro translation using Promega TNT® Coupled Wheat Germ Extract System. (Rabbit reticulocyte lysate could not be used because of luciferase quenching problems). In this reporter gene assay, COS cells were transfected with a firefly luciferase reporter plasmid driven by promoters/enhancers of varying strengths and total cellular RNA was isolated and translated in vitro using the TNT® System. To normalize expression, a CMV Renilla luciferase or beta-galactosidase vector was used as a control. To measure luciferase activity either Promega Luciferase Assay System or Dual-Luciferase® Reporter Assay System was used. (2047)

Expand Full Notes »

J. Biol. Chem. 272, 26620-26626. Regulation of clusterin gene expression by transforming growth factor β. 1997

Jin, G., Howe, P.H.

Notes: Luciferase reporter studies performed in CCL64 (Mv1Lu) mink lung cells using constructs prepared in the pGL2-Basic Vector and were normalized to β-galactosidase activity. Studies performed in 10T1/2, 3Tp, HeLa cells and primary bovine aortic endothelial cells were normalized to the Renilla luciferase. A five to one (w/w) ratio of experimental to control vector was used with pRL-CMV Control Vector. A two to one (w/w) ratio of experimental to control vector was used with pSV-β-Galactosidase Control Vector. (0981)

Expand Full Notes »

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.