Katagiri, Y., Hirata, Y., Milbrandt, J., Guroff, G.
Notes: Dual-Luciferase® Reporter Assay System was used to perform studies in rat PC12 cells. The firefly luciferase reporter vector was transfected with the control plasmid (pRL-TK Vector) at a 20:1 ratio. Eighteen hours after transfection, the cells were treated with NGF, EGF or KCl and assayed up to eight hours later. In some experiments, PC12 cells were co-transfected with the firefly luciferase plasmid, the Renilla control vector and a vector expressing the wildtype or mutant NGFI-B protein. (0920)