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Infect. Immun. 70, 4389–4398. Characterization of Pit, a Streptococcus pneumoniae iron uptake ABC transporter. 2002

Brown, J.S., Gilliland, S.M., Ruiz-Albert, J. and Holden, D.W.

Notes: The authors describe using the Wizard® Genomic DNA Purification kit and the SV Total RNA Isolation System to isolate genomic DNA and total RNA, respectively, from Streptococcus pneumoniae. The researchers also added RNasin® Ribonuclease Inhibitor to purified total RNA before storing it and using it for later RT-PCR reactions performed with the Access RT-PCR System. (2835)

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Plant Physiol. 130, 58–67. Differential expression of a metallothionein gene during the presymbiotic versus the symbiotic phase of an arbuscular mycorrhizal fungus. 2002

Lanfranco, L., Bolchi, A., Ros, E.C., Ottonello, S. and Bonfante, P.

Notes: In this paper, the SV Total RNA Isolation System was used to isolate total RNA from Gigaspora margarita. The authors reported isolating total RNA from 100 spores or 100mg of mycorrhizal roots. One microliter of the isolated RNA was used in RT-PCR to amplify Gigaspora margarita metallothionein (MT)-like polypeptide (GmarMT1) RNA. The researchers also cloned the GmarMT1 coding region using the pGEM®-T Vector. (3077)

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Biochem. J. 372, 347–357. Differential functional properties of calmodulin-dependent protein kinase IIã variants isolated from smooth muscle. 2002

Gangopadhyay, S.S., Barber, A.L., Gallant, C., Grabarek, Z., Smith J.L., and Morgan, K.G.

Notes: Total RNA was isolated from 100mg of ferret aorta tissue using the SV Total RNA Isolation System. One microgram of the purified total RNA was used in RT-PCR to amplify cDNA of CaMKII mRNA. The PCR products were cloned and sequenced. (2851)

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Proc. Natl. Acad. Sci. USA 99, 54–59. Efficient bacterial transcription of DNA nanocircle vectors with optimized single-stranded promoters. 2002

Ohmichi, T., Maki, A. and Kool, E.T.

Notes: Total RNA was isolated from TOP10F’ or INVaF' E. coli (Invitrogen) using the SV Total RNA Isolation System. The isolated RNA was used in RT-PCR to detect cleavage of marA mRNA. A marA drug resistance gene sequence was also ligated into a chloramphenicol acetyltransferase (CAT) reporter vector. This construct was used to measure single-stranded nanovector-transcribed ribozyme activity on the marA sequence. CAT activity was measured using the CAT Enzyme Assay System. CAT activity was normalized by cotransfecting cells with the pSV-β-Galactosidase Vector and assaying lysates with the β-Galactosidase Enzyme Assay System. (2794)

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Physiol. Genomics 10, 181-190. Influence of age, sex, and strength training on human muscle gene expression determined by microarray. 2002

Roth, S.M., Ferrell, R.E., Peters, D.G., Metter, E.J., Hurley, B.F. and Rogers, M.A.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from vastus lateralis muscle biopsies from 20 male and female subjects before and after a nine-week strength-training regime. One microgram of pooled total RNA from each age/sex group was then reverse transcribed to make 33P-labeled cDNAs. Labeled cDNAs from each group were hybridized to GF211 Microarrays (Invitrogen) and analyzed for differential expression patterns. (2759)

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Plant Physiol. 129, 134-144. Isolation and characterization of two germacrene A synthase cDNA clones from chicory plant. 2002

Bouwmeester, H.J., Kodde, J., Verstappen, F., Altug, I.G., Kraker, J.-W. and Wallaart T.E.

Notes: Total RNA was isolated from taproots, etiolated heads, green leaves, and green and etiolated seedlings of chicory using the SV Total RNA Isolation System. Two micrograms of RNA from each sample were used in a Northern blot to analyze gene expression. (2854)

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Appl. Environ. Microbiol. 68, 449–455. Multiple Alternate Transcripts Direct the Biosynthesis of Microcystin, a Cyanobacterial Nonribosomal Peptide. 2002

Kaebernick, M., Dittmann, E., Boerner, T., and Neilan, B.A.

Notes: Total RNA was isolated from Microcystis aeruginosa for use in RT-PCR and RACE (Rapid Amplification of cDNA Ends) to characterize transcripts of a gene cluster encoding microcystin synthetase. (2311)

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Clin. Can. Res. 8, 1794–1799. Reverse transcription-polymerase chain reaction detection of prostate-specific antigen, prostate-specific membrane antigen, and prostate stem cell antigen in one milliliter of peripheral blood: Value of the staging of prostate cancer 2002

Hara, N., Kasahara, T., Kawasaki, T., Bilim, V., Obara, K., Takahashi, K., Tomita, Y.

Notes: The authors used the SV Total RNA Isolation System to purify total RNA from 1 ml of whole blood anticoagulated with EDTA for RT-PCR studies of prostate-specific antigens. (2470)

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J. Neurosci. 21, 934–943. Differing, spatially restricted roles of ionotropic glutamate receptors in regulating the migration of GnRH neurons during embryogenesis. 2001

Simonian, S.X. and Herbison, A.E.

Notes: The SV Total RNA Isolation System was used to isolated RNA from various dissected mouse brain areas. Ten microliters of the isolated RNA were used for RT-PCR analysis of gene expression patterns. (2186)

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Proc. Natl. Acad. Sci. USA 98, 1182–1187. From the Cover: Polycystin-2, the protein mutated in autosomal dominant polycystic kidney disease (ADPKD), is a Ca2+-permeable nonselective cation channel 2001

Gonzalez-Perrett, S., Kim, K., Ibarra, C., Damiano, A.E., Zotta, E., Batelli, M., Harris, P.C., Reisin, I.L., Arnaout, M.A. and Cantiello, H.F.

Notes: The SV Total RNA Isolation System was used to isolated RNA from the syncytiotrophoblast of human term placentas. The isolated RNA was used for RT-PCR with M-MLV Reverse Transcriptase used for the RT reaction. The 110 kDa PKD2 protein was expressed in vitro with the TNT® T7 Coupled Reticulocyte Lysate System with and without Canine Pancreatic Microsomal Membranes. The channel activity of the expressed protein was examined and the functional channel could be inhibited by known inhibitors. Expressing the luciferase control with the membranes did not produce the same effect. (2187)

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Physiol. Genomics 5, 187–192. Microarray analysis of nicotine-induced changes in gene expression in endothelial cells. 2001

Zhang, S., Day, I.N.M. and Ye, S.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from primary human coronary artery endothelial cells. The isolated RNA was used to make gene array 33P-labeled targets on nylon cDNA microarray filters using Oligo(dT) and M-MLV Reverse Transcriptase, RNase H-. The same material was used for first-strand synthesis in RT-PCR. (2697)

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Am. J. Pathol. 158, 373–379. Molecular analysis of mutant and wild-type Tau deposited in the brain affected by the FTDP-17 R406W mutation. 2001

Miyasaka, T., Morishima-Kawashima, M., Ravid, R., Heutink, P., van Swieten, J.C., Nagashima, K. and Ihara, Y.

Notes: The SV Total RNA Isolation System was used to isolated RNA from autopsy samples of human brain with FTDP-17 disorder. The isolated RNA was used for semi-quantitative RT-PCR (2162)

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Antimicrob. Agents Chemother. 45, 1151–1161. Novel Carbapenem-Hydrolyzing β-Lactamase, KPC-1, from a Carbapenem-Resistant Strain of Klebsiella pneumoniae. 2001

Yigit, H., Queenan, A.M., Anderson, G.J., Domenech-Sanchez, A., Biddle, J.W., Steward, C.D., Alberti, S., Bush, K. and Tenover, F.C.

Notes: The study of a carbapenem resistant strain of Klebsiella pneumoniae lead to the description of a novel form of β-lactamase, which confers resistance to imipenem, meropenem, extended-spectrum cepahlosporins, and aztreonam. Total RNA used in a primer extension assay was isolated with the SV Total RNA Isolation System . Primer extension reactions were performed with Promega's Primer Extension System- AMV Reverse Transcriptase to determine the transcriptional start site of the KPC-1 β-lactamase gene. The KPC-1 β-lactamase gene was then sequenced with the fmol® DNA Sequencing System (2300)

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J. Bacteriol. 183, 6801–6806. Transcriptional Regulation of furA and katG upon Oxidative Stress in Mycobacterium smegmatis. 2001

Milano, A., Forti, F., Sala, C., Riccardi, G. and Ghisotti, D.

Notes: Total RNA was isolated from Mycobacterium smegmatis for Northern blot analysis and RT-PCR. Transcripts for furA and katG were amplified by RT-PCR using Promega's MMLV Reverse Transcriptase and cloned into the pGEM®-T Easy vector. Northern blot probes for furA and katG were synthesized by in vitro transcription from the pGEM®-T Easy vector. (2310)

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J. Bacteriol. 182, 2753–2760. 2-Hydroxycyclohexanecarboxyl coenzyme A dehydrogenase, an enzyme characteristic of the anaerobic benzoate degradation pathway used by Rhodopseudomonas palustris. 2000

Pelletier, D.A. and Harwood, C.S.

Notes: Total RNA was isolated from Rhodopseudomonas palustris using the SV Total RNA Isolation System. The Access RT-PCR System was used to determine the transcriptional organization of the badHIaliBA and badK genes, genes involved in anaerobic benzoate degradation. (2306)

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Appl. Environ. Microbiol. 66, 3778–3783. A Serine Protease-Encoding Gene (aprII) of Altermonas sp. Strain O-7 Is Regulated by the Iron Uptake Regulator (Fur) Protein. 2000

Tsujibo, H., Miyamoto, K., Okamoto, T., Orikoshi, H., and Inamori, Y.

Notes: Total RNA was isolated from Alteromonas sp. strain O-7 for Northern blot analysis of the aprII gene to show that aprII is upregulated under iron rich growth conditions. The transcriptional start site of this gene was mapped in a primer extension reaction using Promega's AMV reverse transcriptase. (2304)

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J. Clin. Endocrinol. Metab. 85, 4455–4461. Allelic variants of the folistatin gene in polycystic ovary syndrome. 2000

Urbanek, M., Wu, X., Vickery, K.R., Kao, L.-C., Christenson, L.K., Schneyer, A., Legro, R.S., Driscoll, D.A., Strauss, J.F., III, Dunaif, A. and Spielman, R.S.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from cultured human fibroblasts. The isolated RNA was used for RT-PCR. (2184)

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Pediatr. Res. 48, 748–753. Analysis of exonic mutations leading to exon skipping in patients with pyruvate dehdrogenase E1alpha deficiency. 2000

Cardozo, A.K., de Meirleir, L., Liebaers, I. and Lissens, W.

Notes: The SV Total RNA Isolation System was used to isolate RNA from transiently transfected COS-7 cells bearing expression plasmid for exon 6 from a variety of patients. The isolated RNA was used for RT-PCR analysis. (2185)

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J. Bacteriol. 182, 6339–6346. BenR, A XylS homologue, regulates three different pathways of aromatic acid degradation in Pseudomonas putida. 2000

Cowles, C.E., Nichols, N.N., and Harwood, C.S.

Notes: The authors described a cluster of 8 genes from P. putida that is thought to be involved in benzoate metabolism: benA, benB, benC, benD, benE, benF, benK, and benR. The transcriptional start site of benA was determined using Promega's Primer Extension System-AMV Reverse Transcriptase. Total RNA used in these primer extension reactions was isolated from P. putida cells using the SV Total RNA Isolation System. The transcriptional organization of the benA, benB, and benC genes was determined by RT-PCR using the Access RT-PCR System. (2301)

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FASEB J. 14, 740–751. Cellular dedifferentiation of endothelium is linked to activation and silencing of certain nuclear transcription factors: Implications for endothelial dysfunction and vascular biology. 2000

Thum, T., Haverich, A. and Borlak, J.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from rat endothelial cells. The isolated RNA was used for RT-PCR and semi-quantitative RT-PCR with cDNA generated with AMV Reverse Transcriptase. (2175)

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J. Biol. Chem. 275, 31379–31386. COT Kinase proto-oncogene expression in T cells. Implication of the JNK/SAPK signal transduction pathway in Cot promoter activation. 2000

Sanchez-Gongora, E., Lisbona, C., de Gregorio, R., Ballester, A., Calvo, V., Perez-Jurado, L. and Alemany, S.

Notes: Jurkat cells were fractionated into polysomes, and the resulting polysomes were ethanol precipitated. Total RNA was isolated from the polysomes with the SV Total RNA Isolation System. The system was also used to isolate RNA directly from Jurkat cells without fractionation. The isolated RNA was used for Northern blots, dot blots, RT-PCR and RNase protection assays. (2182)

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Brain Res. Mol. Brain Res. 76, 25–35. Expression of the GDNF family members and their receptors in the mature rat cochlea. 2000

Stöver, T., Gong, T.L., Cho, Y., Altschuler, R.A. and Lomax, M.I.

Notes: Total RNA was isolated from various rat tissues with the SV Total RNA Isolation System. Yields are reported as 10µg from 16 whole cochlea, 8µg from 16 modiola, 10.4µg from 48 cochlear sensorineural epithelial/lateral walls and 50µg from the substantia nigra region of four brains. The isolated RNA was used for RT-PCR in the presence of RNasin® Ribonuclease Inhibitor. The resulting amplimer was subcloned into the pGEM®-T Easy Vector and clones were purified with the Wizard® Plus SV Minipreps System. (2176)

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J. Immunol. 164, 5805–5814. GRID: A novel Grb-2-related adapter protein that interacts with the activated T cell costimulatory receptor CD28. 2000

Ellis, J.H., Ashman, C., Burden, M.N., Kilpatrick, K.E., Morse, M.A. and Hamblin, P.A.

Notes: Total RNA was isolated from 106 actively growing Jurkat, MAW, OZZ or Thp1 cells with the SV Total RNA Isolation System. The equivalent RNA from 8 x 104 cells was used for RT-PCR with the Access RT-PCR System. (2180)

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J. Biol. Chem. 275, 4159–4165. HIV-1 Tat-mediated inhibition of the tyrosine hydroxylase gene expression in dopaminergic neuronal cells. 2000

Zauli, G., Secchiero, P., Rodella, L., Gibellini, D., Mirandola, P., Mazzoni, M., Milani, D., Dowd, D.R., Capitani, S. and Vitale, M.

Notes: The SV RNA Total RNA Isolation System was used to isolate total RNA from rat PC12 cells. The isolated RNA was used for RT-PCR in the Access RT-PCR System. (0083)

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J. Clin. Endocrinol. Metab. 85, 781–792. Human somatostatin receptor subtypes in acromegaly: Distinct patterns of messenger ribonucleic acid expression and hormone suppression identify different tumoral phenotypes. 2000

Jaquet, P., Saveanu, A., Gunz, G., Fina, F., Zamora, A.J., Grino, M., Culler, M.D., Moreau, J.P., Enjalbert, A. and Ouafik, L.H.

Notes: The SV Total RNA Isolation System was used to purify total RNA from 30–60mg of human adenoma tumor tissue. The isolated RNA was used for RT-PCR and a Taqman assay. (2177)

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