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J. Biol. Chem. 274, 20671-20678. Phenotypic analysis of seizure-prone mice lacking L-isoaspartate (D-aspartate) O-methyltransferase 1999

Kim, E., Lowenson, J.D., Clarke, S., Young, S.G.

Notes: The authors created knock out mice, deficient in the gene encoding L-isoaspartate O-methyltransferase in order to determine the biological function of this enzyme. Fibroblasts were obtained from knockout and wildtype mouse embryos and cultured. The cell viability of the cultured fibroblasts was determined using the CellTiter 96® AQueous One Solution Cell Proliferation Assay. (2490)

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J. Biol. Chem. 6, 150-155. Secretory group IIA phospholipase A2 generates anti-apoptotic survival signals in kidney fibroblasts 1999

Zhang, Y., Lemasters, J., Herman, B.

Notes: This paper investigated the role of  group IIA phospholipase A2 (PLA2) in apoptosis and sought to identify its subcellular target. Baby hamster kidney fibroblasts (BHK) were transfected with wildtype or mutant rat PLA2, subjected to growth factor deprivation, and were assayed for survival.  Cells expressing either no PLA2 or mutant PLA2 underwent dramatic apoptosis; cells expressing the wildtype PLA2 showed resistance to apoptosis. The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to determine cell viability of the BHK cells. (2481)

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Proc. Natl. Acad. Sci. USA 96, 669-673. Selective induction of apoptosis by the cytotoxic analog AN-207 in cells expressing recombinant receptor for leuteinizing hormone-releasing hormone. 1999

Danila, D.C., Schally, A.V., Nagy, A., Alexander, J.M.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to assess the survival of COS-7 cells transfected with the leutinizing hormone-releasing hormone receptor. Seventy-two hours after exposure to cytotoxic compounds the cell viability was measured in relation to control cultures. (1253)

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J. Invest. Dermatol. 113, 1090-1098. The function of nitric oxide in wound repair: inhibition of inducible nitric oxide-synthase severely impairs wound reepithelialization. 1999

Stallmeyer, B., Kampfer, H., Kolb, N., Pfeilschifter, J., Frank, S.

Notes: In this paper, the CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to assess the effects of chemicals that donate nitric oxide (NO) on proliferation of the keratinocyte cell line (HaCaT). Cells were cultured for 24hr with the chemicals prior to analysis. (0329)

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Am. J. Physiol. 277, H2038-H2049. VEGF stimulates tyrosine phosphorylation of β-catenin and small-pore endothelial barrier dysfunction. 1999

Cohen, A. W. , Carbajal, J. M. , Schaeffer, R. C. Jr

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to measure the proliferative response of BAEC cells to various concentrations of vascular endothelial cell growth factor (VEGF). The cells were also cultured in the presence of the tyrosine kinase inhibitor herbimycin A or the PKC inhibitor bisindolylmaleimide. Both inhibitors blocked the VEGF-induced proliferation. (1313)

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Blood 91(5), 1625-1632. A proteasome inhibitor, an antioxidant, or a salicylate, but not a glucocorticoid, blocks constitutive and cytokine-inducible expression of P-selectin in human endothelial cells. 1998

Xia, L., Pan, J., Yao, L., and McEver, R. P.

Notes: The MTS-based CellTiter 96® AQueous One Solution Assay was used to monitor the viability of human umbilical vein epithelial cells and bovine aortic endothelial cells following treatment with various pharmacological reagents. (1728)

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Biol. Reprod. 59, 522-526. Epidermal growth factor in the germinal disc and its potential role in follicular development in the chicken. 1998

Volentine, K.K., Yao, H.H.-C., Bahr, J.M.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to assess the proliferation of chicken granulosa layer explants in response to EGF. (0229)

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Mol. Pharmacol. 53 (1), 97-104. Exposure of human vascular smooth muscle cells to Raf-1 antisense oligodeoxynucleotides: cellular responses and pharmacodynamic implications. 1998

Schumacher, C., Cioffi, C. L., Sharif, H., Haston, W., Monia, B. P., and Wennogle, L.

Notes: The MTS-based CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to quantify the anti-proliferative properties of a Raf-1 antisense oligonucleotide. Human coronary artery smooth muscle cells were grown to 80% confluency in 96 well plates and growth-arrested with medium containing no serum or growth factors. The cells were transfected with the antisense oligonucleotides and then media containing 5% serum added. Cells were assayed for various times (24-96 hours) and with various concentrations of antisense oligonucleotide. Promega’s Prime-A-Gene® Labeling System also was used in this publication. (1707)

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Am. J. Respir. Cell Mol. Biol. 17(5), 541-51. Interleukin-4 alters epithelial cell differentiation and surfactant homeostasis in the postnatal mouse lung. 1997

Jain-Vora, S., Wert, S.E., Temann, U.A., Rankin, J.A. and Whitsett, J.A.

Notes: The MTS-based CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to assess the proliferative response of T-cells (20,000 per well) to mitomycin C-treated splenocyte antigen-presenting cells (10,000 per well) and Concanavalin A. T-cells isolated from the lung and spleen of normal mice were compared to transgenic mice expressing IL-4 constitutively in lung tissue. The lung T-cells from the transgenic mice proliferated in response to the antigen-presenting cells. (2091)

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J. Interferon and Cytokine Res. 16(1), 31-33. MTS interferon assay: A simplified cellular dehydrogenase assay for interferon activity using a water-soluble tetrazolium salt. 1996

Khabar, K.S.A., Al-Zoghaibi, F., Dzimiri, M., Taha, M., Al-Tuwaijri, A. and Al-Ahdal, M.N.

Notes: The CellTiter 96® AQueous MTS Reagent Powder was used for assays to measure interferon alpha2a (IFN-alpha2a) inhibition of cytopathic effects (CPE) caused by a challenge of encephalomyocarditis (EMC) virus on WISH cells. (2097)

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