Procollagen C-proteinase enhancer-1 (PCPE-1) interacts with β2-microglobulin (β2-m) and may help initiate β2-m amyloid fibril formation in connective tissues.
Morimoto, H., Wada, J., Font, B., Mott, J.D., Hulmes, D.J., Ookoshi, T., Naiki, H., Yasuhara, A., Nakatsuka, A., Fukuoka, K., Takatori, Y., Ichikawa, H., Akagi, S., Nakao, K. and Makino, H.
Notes: To identify proteins that may initiate the formation of β2-microglobulin (β2-m) amyloid fibrils in dialysis related amyloidoisis (DRA), the authors screened a human synovial cDNA library using β2-m as bait in a yeast two-hybrid system. Procollagen C-proteinase enhancer-1 (PCPE-1) was shown to interact with β2-m. This interaction was confirmed by immunoprecipitation, solid-phase binding and pull-down assays. Full-length PCPE-1 cDNA was cloned into the pTNT™ Vector and expressed using a TNT® Transcription/Translation System. Pull-down assays were performed by immobilizing the GST-β2-m fusion protein using the MagneGST Ni Particles, adding the in vitro translated PCPE-1 protein, washing the particles, then performing SDS-PAGE and Western blotting using anti-PCPE-1 antibodies. (3966)
Expand Full Notes »