Bose, R.N., Maurmann, L., Mishur, R.J., Yasui, L., Gupta, S., Grayburn, W.S., Hofstetter, H. and Salley, T.
Notes: The authors were interested in testing platinum (Pt) anticancer drugs that do not bind to DNA to see how they worked in cisplatin- and carboplatin-resistant cells. The human ovarian cancer cells, A2780 and A2780/C30, were seeded in T75 cm2 flasks with 1.0 × 107 cells. After 24 hours, the cells were treated with 0, 10, 20, 30 and 50µM Pt compounds for 24 hours. Medium was removed, the cells washed, trypsinized and centrifuged. Genomic DNA was isolated using the Wizard® SV Genomic DNA Purification System and quantitated using absorbance at 260nm. This DNA was using in DNA-Pt binding assessment. (4019)