Burstein, E.S., Ott, T.R., Feddock, M., Ma, J.N., Fuhs, S., Wong, S., Schiffer, H.H., Brann, M.R. and Nash, N.R.
Notes: This study examined both the human and the macaque counterparts of the Mas-related genes (Mrg), a family of G-protein-coupled receptors, in assays to compare function and structure. Clones of adenylyl cyclase type II (AC2) and Gαo were subcloned into the pSI Mammalian Expression Vector and used in the co-transfection experiments with the Mrgs. Receptor Selection and Amplification Technology (R-SAT™) was used for this comparison of the human and macaque Mrgs. Cells were plated in 96-well plates and transiently transfected with 57ng of 4 different plasmids including one of the Mrg receptors, 2ng AC2 and 30ng pSV-β-galactosidase Vector. One day post-transfection, ligands were added and after 5 days, the β-galactosidase expression was assessed. (3551)