Paroni, G., Mizzau, M., Henderson, C., Del Sal, G., Schneider, C. and Brancolini, C.
Notes: In this study, the Anti-PARP p85 Fragment polyclonal antibody was used to confirm apoptosis in human IMR90 and MCF-7 cells by Western blotting lysates from UV- treated cells. The Anti-Cytochrome c monoclonal antibody was also used to immunocytochemically stain IMR90 cells that were transiently transfected with various mutant histone deacetylase-4 (HDAC4)-expressing constructs. Data from these experiments was expressed as the percent of cells showing cytochrome c staining in the cytosol. The researchers also mention using a TNT® Coupled Reticulocyte Lysate System to express and label various histone deacetylases (HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, and HDAC6). The translation products were then used in in vitro cleavage assays with recombinant caspase-2 or caspase-3. (3174)