Binder, D.K., Routbort, M.J., Ryan, T.E., Yancopoulos, G.D. and McNamara, J.O.
Notes: To test the efficacy of TrkA, B and C-IgG fusions to their respective ligands, PC12 cells were stimulated Nerve Growth Factor (NGF) and TrkA-IgG, TrkB-IgG, or TrkC-IgG. Only the TrkA-IgG blocked tyrosine phosphorylation as judged by a pan phosphoTrk antibody. The TrkB-IgG blocked the activity of Brain-Derived Neurotrophic Factor (BDNF) on cortical cells and TrkC-IgG blocked Neurotrophin-3 (NT-3) action. Promega's BDNF, NT-3 and NGF, 2.5S, were used. (1452)