Debacq-Chainiaux,F., Borlon, C., Pascal, T., Royer, V., Eliaers,F., Ninane, N., Carrard, G., Friguet, B., de Longueville, F., Boffe, S., Remacle, J. and Toussaint, O.
Notes: The authors of this study developed a model for UVB-induced premature senescence of skin human diploid fibroblasts. Markers of senescence were expressed in the system, and the authors were able to detect a common mitochondrial DNA 4,977-bp deletion that is associated with oxidative damage. After a series of ten UVB stresses, total RNA was prepared from the cells using the RNAgents® Total RNA Isolation System and used for RT-PCR to detect differentially expressed genes. Forty-four stress or senescence-associated genes were identified that were differentially expressed between UVB irradiated and untreated cells including c-fos, c-jun, insulin-like growth factor binding protein 3, several HSPs, genes involved in protection from oxidative stress, and the type II receptor of TGF-β. The Anti-ACTIVE® Caspase-3 pAb was used to assess whether the UVB treatment or incubation with TGF-β1 led to apoptosis in this system. (3670)