Canto, P., Vilchis, F., Soderlund, D., Reyes, E. and Mendez, J.P.
Notes: In this study, coding sequence abnormalities in desert hedgehog (DHH), a gene involved in male sex differentiation, were investigated. Genomic DNA was isolated from paraffin-embedded gonadal tissue from individuals with mixed gonadal dysgenesis and from three controls using the MagneSil® Genomic, Fixed Tissue System. The purified DNA was then analyzed by exon-specific PCR, single-stranded conformation polymorphism (SSCP) analysis, and sequencing. (3447)