We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

HaloLink™ Resin: High Capacity, Specificity and Scalable Throughput for Protein Analysis

  • Print
  • Email
  • Download PDF


HaloLink™ Resin provides a new method for specific, covalent and oriented immobilization of proteins onto surfaces. The strategy is based on the HaloTag® Protein, which is derived from a catalytically inactive hydrolase engineered to form a covalent bond with a specific ligand. Here we demonstrate the high binding capacity and minimal nonspecific binding of the HaloLink™ Resin. We also show that the covalent HaloTag® bond provides stability during dilution and stringent washing, minimizing the loss of the HaloTag® fusion proteins from the surface. By analyzing protein:protein interactions and enzyme activity for several fusion proteins, we also demonstrate that fusion proteins bound to the HaloLink™ Resin maintain functionality. HaloLink™ Resin is not a purification resin; however, in conjunction with protease cleavage, it can be used to isolate a protein of interest.

Promega Notes 92, 24–29.

Marjeta Urh1, Dan Simpson1, Jacqui Sankbeil1, Danette Hartzell1, Natasha Karassina1, Natalie Betz1, Nadine Nassif1, Jami English1, Ji Zhu2, Poncho Meisenheimer2, Dieter Klaubert2, Bob Bulleit1, Keith Wood1

1Promega Corporation, 2Promega Biosciences, Inc.
Publication Date: 2006

Download Article (310 KB)

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.