Instructions for Use of Product(s)
Literature # TB127
The Flexi® Rabbit Reticulocyte Lysate System is widely used to identify mRNA species and characterize their products. This eukaryotic system has been reported to post-translationally modify proteins via phosphorylation, acetylation and isoprenylation. Signal peptide cleavage and core glycosylation also can be achieved by the Flexi® Rabbit Reticulocyte Lysate System with addition of Canine Pancreatic Microsomal Membranes. The system provides greater flexibility of reaction conditions than standard rabbit reticulocyte lysate systems. As with the standard Rabbit Reticulocyte Lysate, the Flexi® Rabbit Reticulocyte Lysate System is optimized for translation by addition of:
- Hemin, to prevent inhibition of initiation factor eIF-2α
- An energy-generating system consisting of pretested phosphocreatine kinase and phosphocreatine
- Calf liver tRNAs, to balance the accepting tRNA populations, thus optimizing codon usage and expanding the range of mRNAs that can be translated efficiently
- Micrococcal nuclease, to eliminate endogenous mRNA, thus reducing background translation
Summary of Changes
The 4/15 version of this Technical Bulletin was revised to update patent and disclaimer statements.
Printed in USA. Revised 4/15.
Experienced User Protocols