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Promega Corporation

pF4A CMV Flexi® Vector Product Information

  • Instructions for Use of Product(s)
  • C8481

Literature # 9PIC848

The pF4A CMV Flexi® Vector is designed for use with the Flexi® System, Entry/Transfer (Cat.# C8640), and the Flexi® System, Transfer (Cat.# C8820). The vector contains a CMV immediate early enhancer/promoter plus a chimeric intron for mammalian expression of the protein-coding region and a T7 promoter for in vitro protein expression of the protein-coding region. The vector also contains the lethal barnase gene for positive selection of the insert, an ampicillin-resistance gene for selection of the plasmid and unique SgfI and PmeI sites that allow easy insertion or transfer of the sequence of interest. Inserts containing a protein-coding region can easily be transferred from the pF4A CMV Flexi® Vector to other Flexi® Vectors with different expression options.

Printed in USA. Revised 9/13.

 

PDF Icon Complete Protocol PDF (198kb)

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