Technical Articles By Topic Area

This is a list of technical articles that were previously published in Promega Notes, Cell Notes or eNotes. The articles are organized by topic area. To find articles by issue, visit the magazine archives, which are linked in the left-hand column.

Automation

Maxwell® 16 Systems

Automate the Apo-ONE®: Homogeneous Caspase-3/7 Assay on the Eppendorf epMotion®: 5075 TMX


The Rise of Personal Automation

Automated DNA Purification from Oragene•DNA Saliva Samples Using the Maxwell® 16 System

Have You Seen the Maxwell® 16 Applications Database?

Search the New Maxwell® 16 Applications Database for Specific Sample Information

Personal Automation™ for Increased Productivity


New Maxwell® 16 Instrument Accessories Increase the Versatility of Your Maxwell® 16 System

Maxwell® 16 Buffy Coat Genomic DNA Purification Application

Maxwell® 16 Tissue and Cell Total RNA LEV Systems

The Versatility of the Maxwell® 16 System for Genomic DNA Extraction

Purification of Genomic DNA from Mouse Feces Using the Maxwell® 16 System


Introducing the Maxwell™ 16 Instrument: A Simple, Robust and Flexible Tool for DNA Purification

Other Systems

Perform Automated Nucleic Acid Purification Using the SV Systems and the epMotion® 5075 VAC Workstation

High-Throughput Compound Profiling Using Tecan Instrumentation

Ultrahigh-Throughput Compound Profiling Using Next-Generation Dispensing Technology

MagneSil® Genomic, Large Volume System, for Large-Sample Genomic DNA Isolation

Automating Cell-Based Assays in Multiwell Formats


High-Throughput DNA Fragment Purification Using the MagneSil® Automated 384-Well Clean-Up Systems

Automated 96-Well Purification of Genomic DNA from Whole Blood

Automation of Genomic DNA Purification Using the MagneSil® KF, Genomic System

Automating Promega Cell-Based Assays in Multiwell Formats

PolyATtract® Automated System for Isolation of Poly(A)+ mRNA


Automated Polyhistidine-Tagged Protein Purification Using the MagneHis™ Protein Purification Sys

Automating the Wizard® Magnetic 96 DNA Plant System on Thermo Labsystems’ Kingfisher®

Automated Purification of PCR Products Using MagneSil™ Paramagnetic Particles

Automated Genomic DNA Purification Using the Wizard® Magnetic 96 DNA Plant System

High-Throughput Purification Using the SV 96 Total RNA Isolation System


Automated Poly(A)+ mRNA Isolation From Plant Tissues Using the PolyATtract® System 1000

Automated Isolation of Genomic DNA Using Promega’s Wizard® SV 96 DNA Binding Plates on the Beckman Biomek® 2000

Automated Isolation of Genomic DNA Using Promega's DNA Binding Plates on the Beckman Biomek® 2000

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Cellular Health

Apoptosis

Automate the Apo-ONE®: Homogeneous Caspase-3/7 Assay on the Eppendorf epMotion®: 5075 TMX

Determining the Predictive Mechanism of Toxicity Using a Single-Well Multiplexed Assay

Sensitive and Flexible Protease Assay without the Need for Chemical Synthesis


Using the GloMax®-Multi Detection System for Measuring Signal from Multiplexed Fluorescent and Luminescent Assays

Choosing the Right Assay to Monitor Your Signal Transduction Pathway

Citation Note: Measuring Caspase-3/7 Activity in Serum Using a Luminescent Assay

New, Sensitive Bioluminescent Caspase-Glo® 2 and 6 Assays: Using Inhibitors to Achieve Specificity in Cells

Using Caspase-Glo® Assays to Perform Whole Organism Screening for Apoptosis in Zebrafish Embryos


Improve the Quality and Specificity of Cell-Based Caspase-Glo® 8 and 9 Assays with a Protease Inhibitor

Custom Enzyme Substrates for Luciferase-Based Assays

Timing Your Apoptosis Assays

Multiplexed Viability, Cytotoxicity And Apoptosis Assays For Cell-Based Screening

High-Throughput Compound Profiling Using Tecan Instrumentation


Ultrahigh-Throughput Compound Profiling Using Next-Generation Dispensing Technology

Citation Note: Development of a Homogeneous Bioluminescent Caspase Assay

Citation Note: Detecting Caspase Activities in Mouse Liver

Multiplexing Homogeneous Cell-Based Assays

Miniaturizing and Automating Cell Viability and Reporter Assays for High-Throughput and Ultrahigh-Throughput Screening


Correlation of Caspase Activity and Chemo-Response in Epithelial Ovarian Cancer Cell Lines

Technically Speaking: Cell-Based Caspase Assays: Analyzing the Data

Automating Promega Cell-Based Assays in Multiwell Formats

Detecting UV Irradiation-Induced Apoptosis with the Caspase-Glo™ 3/7 Assay

Characterizing Responses to Treatments Using Homogeneous Caspase Activity and Cell Viability Assays


Citation Note: Using the Apo-ONE® Assay in Tissue Extracts and Using Luciferase Reporters to Study Hypoxia-Induced Gene Expression

Detect Caspase-8 and -9 Activities Using the Caspase-Glo™ Assays

Detecting Caspase Activity in Staurosporine-Treated Human Neuroblastoma Cells Using Fluorescent and Luminescent Methods

Caspase-Glo® 3/7 Assay: Use Fewer Cells and Spend Less Time with this Homogeneous Assay

Apoptosis Detection Using the Acumen Explorer™ ADS and Promega's Anti-PARP p85 Fragment Antibody


Detecting Caspase Activities in Mouse Liver

Promega's Anti-PARP p85 Fragment Antibody for Apoptosis Detection

Using Terminal Deoxynucleotidyl Transferase (TdT) Enzyme to Detect TUNEL-Positive, GFP-Expressing Apoptotic Cells

Human ΔNp73 Regulates a Dominant Negative Feedback Loop for TAp73 and p53

Apo-ONE™ Homogeneous Caspase-3/7 Assay: Robust, High-Throughput Apoptosis Detection


Technically Speaking: Cell Viability and Apoptosis Assays

Demonstration of Immunohistochemical Staining Using Promega's Anti-ACTIVE® and Apoptosis Antibodies

The Apo-ONE® Homogeneous Caspase-3/7 Assay: A Simplified "Solution" for Apoptosis Detection

An Integrated Approach to Studying Apoptosis: From Gene Expression to Cellular Events

A Herpes Simplex Virus Type 2 Protein (ICP10 PK) Inhibits Caspase-3 Activation in Hippocampal Neurons


An Alternative, Non-Apoptotic Form of Programmed Cell Death

Polycystin-1, the Gene Product of PKD1, Induces Resistance to Apoptosis and Spontaneous Tubulogenesis in MDCK Cells

Immunohistochemical Staining Using Promega Anti-ACTIVE® and Apoptosis Antibodies

Promega Apoptosis Products

Citation Note: Akt-Mediated Survival of Oligodendrocytes Induced by Neuregulins


Anti-ACTIVE® Caspase-3 pAb for the Detection of Apoptosis

CaspACE™ FITC-VAD-FMK In Situ Marker for Apoptosis: Applications for Flow Cytometry

Sensitivity of the Fluorometric and Colorimetric CaspACE™ Assay Systems and Purification of Fragmented DNA from Apoptotic Cells

Viability and Cytotoxicity

Doing Good Science: Authenticating Cell Line Identity (2009)

Expanding Applications of Bioluminescence Technology: Predictive Bioassays for TNFα Biologicals Potency and Dose-Standardization Studies

Homogeneous Luminescence-Based Assay for Quantifying the Glutathione Content in Mammalian Cells

Doing Good Science: Authenticating Cell Line Identity (2008)

Use of the BacTiter-Glo™ Microbial Cell Viability Assay to Study Bacterial Attachment in Biofilm Formation


NIH Chemical Genomics Center: Small-Molecule Screening for Investigating Fundamental Biological Questions

Application of the BacTiter-Glo™ Assay for Rapid Enumeration and Screening of Antimicrobial Compounds for Mycobacterium avium Complex Bacteria

Choosing the Right Assay to Monitor Your Signal Transduction Pathway

High-Throughput Automation of Multiplexed Cell-Based Assays for Viability and Cytotoxicity

Detecting Toxicological Responses in Cells with the Bioluminescent GSH-Glo™ Glutathione Assay


Monitoring Viability During Gene-Directed Enzyme Prodrug Therapy with the CellTiter-Glo® Assay and Human Neural Progenitor Cells

Application of Bioluminescence and Cell-Based Assays in High-Throughput Screening Studies

Assessing Cell Viability in Primary Cultures Using a Luminescent ATP-Based Assay: Applications for the CellTiter-Glo® Assay

Using Protease Biomarkers to Measure Viability and Cytotoxicity

Measure Relative Numbers of Live and Dead Cells and Normalize Assay Data to Cell Number


CellTiter-Glo® Luminescent Cell Viability Assay: Application for Viability Studies of Cells Grown in Agarose

Multiplexed Viability, Cytotoxicity And Apoptosis Assays For Cell-Based Screening

MultiTox-Fluor Multiplex Cytotoxicity Assay Technology

A Complete Solution for Measuring Biological and Cellular Activities: GloMax® Luminometers and Promega Bioluminescent Assays

BacTiter-Glo™ Assay for Antimicrobial Drug Discovery and General Microbiology


Selecting Cell-Based Assays for Drug Discovery Screening

Perform Multiplexed Cell-Based Assays on Automated Platforms

Quantitate Microbial Cells Using a Rapid and Sensitive ATP-Based Luminescent Assay

Multiplexing Homogeneous Cell-Based Assays

Miniaturizing and Automating Cell Viability and Reporter Assays for High-Throughput and Ultrahigh-Throughput Screening


Determining Microbial Viability Using a Homogeneous Luminescent Assay

Automating Promega Cell-Based Assays in Multiwell Formats

Choosing the Right Cell-Based Assay for Your Research

Frequently Asked Questions: CytoTox-ONE™ Homogeneous Membrane Integrity Assay

CellTiter-Glo® Assay: Application for Assessing Direct Cytotoxicity and for Determining Cell Proliferation


The CellTiter™-Blue Cell Viability Assay: Monitoring Cell Viability Using a Fluorescent Redox Indicator Dye

Introducing the CellTiter-Blue™ Cell Viability Assay

CellTiter-Glo® Luminescent Cell Viability Assay for Cytotoxicity and Cell Proliferation Studies

ATCC Verifies Cell Line Identity Using Promega Reagents

CytoTox-ONE™ Homogeneous Membrane Integrity Assay: A Tool for Automated Cytotoxicity Research


Introducing the CytoTox-ONE™ Homogeneous Membrane Integrity Assay

In Vitro Toxicology and Cellular Fate Determination Using Promega's Cell-Based Assays

CellTiter-Glo® Luminescent Cell Viability Assay: Fast, Sensitive and Flexible

Technically Speaking: Cell Viability and Apoptosis Assays

Bringing Automation to the Cytotoxicity Researcher: An ADME/Tox Application


The Predictive Nature of High-Throughput Toxicity Screening Using a Human Hepatocyte Cell Line

CellTiter-Glo® Luminescent Cell Viability Assay: A Sensitive and Rapid Method for Determining Cell Viability

High-Throughput Screening with the CellTiter-Glo® Luminescent Cell Viability Assay

A High-Throughput System for Screening Potential Antiviral Compounds Using the CellTiter 96® AQueous One Solution Assay

Improved Non-Radioactive Assay to Measure Cellular Proliferation or Toxicity: The CellTiter 96® AQueous One Solution Cell Proliferation Assay


Technically Speaking CellTiter 96® Non-Radioactive Cell Proliferation Assays and CytoTox 96® Non-Radioactive Cytotoxicity Assay

Use of Promega's CytoTox 96® Non-Radioactive Cytotoxicity Assay for the Study of MHC Class I Presentation of an Exogenous Antigen

Using Promega's CytoTox 96® Non-Radioactive Cytotoxicity Assay to Measure Cell Death Mediated by NMDA Receptor Subunits

Use of the CytoTox 96® Assay in Routine Biocompatibility Testing In Vitro

Total Cell Quantitation Using the CytoTox 96® Non-Radioactive Cytotoxicity Assay


CellTiter 96® and CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assays

Comparison of attachment factors using the CellTiter 96® Assay

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Cloning

Value-Added Human Flexi® ORF Clones with HaloTag® Labeling Technology


How to Construct and Use a HaloTag® Coding Region Control Expression Vector

Comparing the Cloning Efficiency of the pGEM®-T and pGEM®-T Easy Vectors to the TOPO TA Cloning® Vectors

Recombinant Clone Screening Using the GoTaq® Hot Start Green Master Mix

Sequencing Primers for Flexi® Vector Inserts

TSAP Thermosensitive Alkaline Phosphatase Activity in Restriction Enzyme Buffers from New England Biolabs


Rapid DNA Digestion Using Promega Restriction Enzymes

Comparison of the Flexi® Vector Systems to the Gateway® Cloning System
From the article: Blommel, P.G. et al. (2006) Protein Expr. Purif. 47, 562–70.

TSAP:A New Thermosensitive Alkaline Phosphatase

The Flexi® Vector Systems: The Easy Way to Clone

Detecting Pituitary Adenylate Cyclase-Activating Polypeptide in Cartilaginous Fish Brain


Clone and Express Protein-Coding Regions Using the Flexi® Vector Systems

A New System for Cloning and Expressing Protein-Coding Regions

Technically Speaking: T-Vector Cloning

Work Smarter Using Isoschizomers and Neoschizomers

Technically Speaking: Subcloning Plasmid DNA Constructs


pGEM®-T Easy Vector System is an Easy Tool for Preparing Gel Shift Probes

Constructing Genomic Libraries Using the pGEM®-T Vector

Rapid Ligation for the pGEM®-T and pGEM®-T Easy Vector Systems

Cloning Blunt-End Pfu DNA Polymerase-Generated PCR Fragments into pGEM®-T Vector Systems

Technically Speaking: Optimized Cloning with T Vectors


Stability of pGEM®-T Vectors

Cloning Blunt-End DNA Fragments into the pGEM®-T Vector Systems

Amplification of Flanking Regions: New Applications and Performance Optimization of Single Specific Primer PCR and T-Vector Cloning

pTARGET™ Vector: A New Mammalian Expression T-Vector

pGEM®-T Vector: Cloning of Modified Blunt-Ended DNA Fragments


Technically Speaking: pGEM®-T Vector System

Technically Speaking: RiboClone® cDNA Synthesis Systems and Related Cloning Products

pGEM®-T Vector Systems Troubleshooting Guide

Genomic Cloning Troubleshooting: Evaluating the Success of Partial Fill-In Reactions with the XhoI Half-Site Arms Cloning Strategy


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DNA Purification

Genomic

Automated Purification of DNA from Mouse Tail Clippings Using the Maxwell® 16 Instrument

Automated DNA Purification from Oragene•DNA/Saliva Samples Using the Maxwell® 16 System

A Modified Wizard® SV Genomic DNA Purification System Protocol to Purify Genomic DNA from Shed Reptile Skin

Perform Automated Nucleic Acid Purification Using the SV Systems and the epMotion® 5075 VAC Workstation

Search the New Maxwell® 16 Applications Database for Specific Sample Information


Maxwell® 16 Buffy Coat Genomic DNA Purification Application

The Versatility of the Maxwell® 16 System for Genomic DNA Extraction

Using the Wizard® Plus SV Minipreps DNA Purification System to isolate Rikettsia DNA
From the article: Giammanco, G.M. et al. (2005) J. Clin. Microbiol. 43, 6027–31.

Purification of Genomic DNA from Mouse Feces Using the Maxwell® 16 System

Introducing the Maxwell™ 16 Instrument: A Simple, Robust and Flexible Tool for DNA Purification


DNA Isolation From Lambda Lysates Using the Wizard® DNA Clean-Up System

Isolation of DNA from Bacillus subtilis Using the Wizard® Plus SV Miniprep DNA Purification System

MagneSil® Genomic, Large Volume System, for Large-Sample Genomic DNA Isolation

Citations Isolating Bacterial DNA with the Wizard® Genomic DNA Purification Kit

Wizard® Genomic DNA Purification Kit Provides High-Quality Genomic DNA Template for Molecular Phylogenetic Studies on Copepod Crustaceans


Automated 96-Well Purification of Genomic DNA from Whole Blood

Automation of Genomic DNA Purification Using the MagneSil® KF, Genomic System

PCR-Ready Genomic DNA Isolation From Hair Samples Using the ReadyAmp™ Genomic DNA Purification System

Isolation of Genomic DNA from Bacterial Cells using the Wizard® SV Genomic DNA Purification System

Promega's SV Membrane Technology: The Evolution of an Indispensable Laboratory Tool


Introducing the Wizard® SV and SV 96 Genomic DNA Purification Systems

Isolation of Genomic DNA from Agricultural Bacteria Using the Wizard® MagneSil™ Plasmid Purification System

Automated Isolation of Genomic DNA Using Promega’s Wizard® SV 96 DNA Binding Plates on the Beckman Biomek® 2000

Wizard® Genomic DNA Purification Kit and the Isolation of Plant Genomic DNA

Automated Genomic DNA Purification Using the Wizard® Magnetic 96 DNA Plant System


Using the Wizard® Genomic DNA Purification Kit with 96 Well Plates

Isolation of Genomic DNA from Small Volumes of Whole Blood Using the Wizard® Genomic DNA Purification Kit

Extraction and Amplification of DNA from an Ancient Moss

Rapid Isolation of Genomic DNA from Human Samples: Forensics and Molecular Diagnostics Applications

Postmortem Isolation and Analysis of Human Genomic DNA from HIV-Infected Tissues: A Molecular Diagnostics Application


Separate Isolation of Genomic DNA and Total RNA from Single Samples Using the SV Total RNA Isolation System

Molecular Diagnostics: Isolation and Analysis of Human Genomic DNA

Isolation of Spirochete DNA Using the Wizard® Genomic DNA Purification Kit

Rapid Isolation of High Quality Genomic DNA from Various Sources using the Wizard® Genomic DNA Purification Kit


Plasmid

The effects of decreasing elution volumes on plasmid DNA concentration and yield using Eluator™ Vacuum Elution Device

PureYield™ Plasmid Purification Systems Comparison: Excellent Speed, Yield, Purity and Performance


Remove the High-Speed Spin from PureYield™ Plasmid Preps

Transfection-Quality Plasmid DNA in as Little as Ten Minutes Using the PureYield™ Plasmid Miniprep System

Fast, Reliable, High-Quality Midiprep Plasmid Purification Using the PureYield™ Plasmid Midiprep System

Yeast Plasmid Isolation Using the Wizard® Plus SV Minipreps DNA Purification System

Wizard MagneSil Tfx™ System for the Purification of Transfection-Grade DNA


Automated Plasmid Purification Using MagneSil™ Paramagnetic Particles

High-Throughput Solutions for DNA Purification Using MagneSil™ Paramagnetic Particles

A Simple and Reproducible Method to Isolate Plasmid DNA from Yeast after a Two-Hybrid Screening

Wizard® SV 96 Plasmid DNA Purification System: High Quality Plasmid DNA for Use in Fluorescent Sequencing Methods

Wizard® Plus Minipreps for the Isolation of Binary Plasmids from Agrobacterium tumefaciens


MagneSil™ Paramagnetic Particles: Novel Magnetics for DNA Purification

Wizard® Plus SV Minipreps DNA Purification System: The Next Generation in Miniprep Purification

Technically Speaking: Wizard® Plus DNA Purification System

Wizard® Series 9600™ DNA Purification System Enhancements

Effects of Bacterial Strains Carrying the endA1 Genotype on DNA Quality Isolated with Wizard® Plasmid Purification Systems


Fragment

High-Throughput DNA Fragment Purification Using the MagneSil® Automated 384-Well Clean-Up Systems

Wizard® SV Gel and PCR Clean-Up System

Introducing the Wizard® SV 96 PCR Clean-Up System

Technically Speaking: Microarrays

High-Throughput Solutions for DNA Purification Using MagneSil™ Paramagnetic Particles


MagneSil™ Paramagnetic Particles: A High-Throughput PCR Purification System for Microarrays

Automated Purification of PCR Products Using MagneSil™ Paramagnetic Particles

Removal of Ethidium Bromide and Calf Intestinal Alkaline Phosphatase Using the Wizard® SV Gel and PCR Clean-Up System

Removal of Ethidium Bromide from DNA Fragments Using Wizard® DNA Clean-Up or Wizard® PCR Preps DNA Purification Systems

Purification of Fragmented Apoptotic DNA Using the Wizard® Plus Series 9600™ DNA Purification System


Technically Speaking: Wizard® Plus DNA Purification Systems

Efficient Purification of Multiple PCR Reactions or DNA Fragments from Agarose

Large-scale Gel Purification of DNA Fragments using Wizard™ PCR Preps Resin


Mitochondrial (mtDNA)

Purification of Mitochondrial DNA with Wizard® Minipreps DNA Purification System


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Drug Targets

Cytochromes P450

Six Bioluminescent Substrates for P450 Enzyme Assays

CYP3A4 P450-Glo™ Assays with Luciferin-IPA: The Most Sensitive and Selective Bioluminescent CYP3A4 Assay

Luminogenic Enzyme Substrates: The Basis for a New Paradigm in Assay Design (for Proteases and Cytochrome P450 Enzymes)

Selective Cytochrome P450 4A11 Enzyme Assay Using a Novel Bioluminescent Probe Substrate

Cytochrome P450 4F2 and 4F3B Enzyme Assays Using a Novel Bioluminescent Probe Substrate


Cytochrome P450 4F12 Enzyme Assay Using a Novel Bioluminescent Probe Substrate

Selective Cytochrome P450 3A7 Enzyme Assay Using a Novel Bioluminescent Probe Substrate

Cytochrome P450 2J2 Enzyme Assay Using a Novel Bioluminescent Probe Substrate

Nonselective Cytochrome P450 Enzyme Assay Using a Novel Bioluminescent Probe Substrate that Cross-reacts with Multiple P450 Enzymes

Rat Hepatocyte Culture Physiology Shows Enhanced Cytochrome P450 Activity on a Synthetic Extracellular Matrix


P450-Glo™ CYP3A4 Biochemical and Cell-Based Assays

Custom Enzyme Substrates for Luciferase-Based Assays

In Vitro Compound Profiling in 384- and 1536-Well Formats: Using Bioluminescent ADME Assays to Understand In Vivo Biology

High-Throughput Compound Profiling Using Tecan Instrumentation

Screen For Cytochrome P450 Activity Using a Luminescent Assay


Miniaturizing Luminescent P450-Glo™ Assays for HTS

Screen for CYP450 Inhibitors Using P450-Glo™ Luminescent Cytochrome P450 Assays

GPCR

Monitor GPCR Modulation of Cellular cAMP with an HTS, Bioluminescence-Based Assay

Choosing the Right Assay to Monitor Your Signal Transduction Pathway

NIH Chemical Genomics Center: Small-Molecule Screening for Investigating Fundamental Biological Questions

Using Luciferase Reporter Assays to Screen for GPCR Modulators

Using Luciferase Assays to Study G-Protein Coupled Receptor Pathways and Screen for GPCR Modulators

Kinases (and phosphatases)

Novel Biosensors to Monitor Cellular Events in Live Cells

Studying MAP Kinase Signaling with a Small-Molecule Inhibitor, U0126

NIH Chemical Genomics Center: Small-Molecule Screening for Investigating Fundamental Biological Questions

Choosing the Right Assay to Monitor Your Signal Transduction Pathway

Screen for Kinase Modulators in a High-Throughput Format with Promega Kinase Reagents


Optimizing Kinase Assays for Ultrahigh-Throughput Profiling Using the Kinase-Glo® Plus Assay

Small-Molecule Kinase Inhibitors: From Lab Bench to Clinic

Choosing the Best Kinase Assay to Meet Your Research Needs

Assay Virtually Any Kinase With Kinase-Glo® Plus Luminescent Kinase Assay,  A Homogeneous High-Throughput Assay

Citation Note: Measuring LPS-Induced PKC Activity in U937 Cells


High-Throughput Kinase Screening Using a Universal Luminescent Kinase Assay

Assay Protein Tyrosine Kinase and Protein Tyrosine Phosphatase Activity in a Homogeneous, Non-Radioactive High-Throughput Format

Monitor Purified Phosphatase Activity with a Homogeneous, Non-Radioactive High-Throughput Fluorogenic Assay

ProFluor™ PKA Assay: Excellent Z´-Factor Values Mean Reliable Results

Introducing the Kinase-Glo® Luminescent Kinase Assay


Kinase-Glo® Luminescent Kinase Assay: Detect Virtually Any Kinase

From One to 9,000 Samples: Using High-Density Streptavidin-Coated Membranes for Kinase Detection

Scaffold Proteins in Mammalian JNK Signaling

Citation Note: The p44/42 Mitogen-Activated Protein Kinase Cascade is Involved in the Induction and Maintenance of Astrocyte Stellation Mediated by Protein Kinase C

Tools to Study the Activation of CaM KII in Neuronal Functions


Technically Speaking: Anti-ACTIVE® Antibodies and MAPK Signaling Pathways

Using InCELLect™ Cell-Permeable, Stearated Peptides to Probe cAMP-Dependent Protein Kinase-Mediated Cellular Signaling Reactions In Vivo

Protein Kinases as Drug Targets in High-Throughput Systems

A Strategy for Survival: The Akt Signaling Cascade

PC12 Cell Extracts for Use with the New Anti-pT183 MAPK and Anti-ACTIVE® Antibodies


U0126: An Inhibitor of MKK/ERK Signal Transduction in Mammalian Cells

U0126: A Novel, Selective and Potent Inhibitor of MAP Kinase Kinase (MEK)

New Anti-ACTIVE® MAPK & 'pan ERK 1/2' Antibodies for Western Analysis

Anti-ACTIVE® Antibody for Specific Detection of Phosphorylated CaM KII Protein Kinase

Inhibition of EGF-Induced ERK1 and ERK2 Enzyme Activation in NIH3T3 Cells by in situ Electroporation of a Nonpermeant Inhibitor


Advances in the SAM2® Membrane Technology: High Throughput Biotin Capture Systems for Use in Rapid Screening

Signal Integration and Activation of MAP, JNK and p38 Kinase Pathways in 293 Cells

A Comparison of Western Blotting Techniques Using Phospho-Specific Antibody Detection of Protein Kinase Activation

Technically Speaking Anti-ACTIVE® Antibodies to MAPK, JNK and p38

SignaTECT® DNA-Dependent Protein Kinase Assay System


Detection of JNK, p38 and MAPK Enzyme Activation Using Anti-Dual-Phosphopeptide Antibodies: Coordinated Signaling of the Extracellular Signal-Regulated Protein Kinase (ERK) Superfamily

Detection and Quantitation of Protein Tyrosine Kinases

MAP Kinase Signaling Pathways

Detection of MAP Kinase Activation Using Anti-ACTIVE® MAPK pAb

SAM2® Biotin Capture Membrane and SignaTECT® Protein Kinase Assay Systems


Specific Detection of Biologically Active MAP Kinase using Anti-ACTIVE® MAPK pAb

A Novel Method for Non-Radioactive Assays of Specific Protein Kinases

Monoamine Oxidase (MAO)

The MAO-Glo™ Assay: A Bioluminescent-Coupled Assay for Monoamine Oxidase Activity

In Vitro Compound Profiling in 384- and 1536-Well Formats: Using Bioluminescent ADME Assays to Understand In Vivo Biology

Custom Enzyme Substrates for Luciferase-Based Assays

The MAO-Glo™ Assay: A Bioluminescent-Coupled Assay for Monoamine Oxidase Activity

Nuclear Receptors and Transcription Factors

Expanding Applications of Bioluminescence Technology: Predictive Bioassays for TNFα Biologicals Potency and Dose-Standardization Studies

Citation Note: Measuring NF-κB Activity Using Two-Color, Dual-Luciferase Reporters

Screening Traditional Chinese Medicines Against Estrogen Receptors α and β

NIH Chemical Genomics Center: Small-Molecule Screening for Investigating Fundamental Biological Questions

Other Enzymes and Transporters

Detecting the Deubiquitin Activity of SARS-CoV PLpro: Turning on the Light with the DUB-Glo™ Protease Assay

Identify P-glycoprotein Substrates and Inhibitors with the Rapid, HTS Pgp-Glo™ Assay System

Bioluminescent N-Acetyl Transferase-2 Enzyme Assay Using a Novel Luciferin Derivative as a Substrate

Proteases

Detecting the Deubiquitin Activity of SARS-CoV PLpro: Turning on the Light with the DUB-Glo™ Protease Assay

Sensitive and Flexible Protease Assay without the Need for Chemical Synthesis

Detection of Dipeptidyl Peptidase with DPPIV-Glo™ Assay

New Bioluminescent Cell-Based Assays to Measure All Three Proteasome Protease Activities

Screen for Calpain Inhibitors Using a Cell-Based, High-Throughput Assay

Custom Enzyme Substrates for Luciferase-Based Assays

DPPIV-Glo™ Protease Assay: A More Sensitive Method For Measuring Gly-Pro Cleaving Activity In Serum


Monitoring Proteasome Activity with a Cell-Based Assay Using a Single-Addition Luminescent Method

Assay for DPPIV Activity Using a Homogeneous, Luminescent Method

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Food & GMO Testing

ATP Measurement as a Means for Directly Estimating Active Biomass

Quantitate Microbial Cells Using a Rapid and Sensitive ATP-Based Luminescent Assay

Wizard® Magnetic DNA Purification System for Food: Part I. DNA Isolation and Analysis of GMO Foods by PCR

Wizard® Magnetic DNA Purification System for Food: Part II. Semi-Automated DNA Isolation and Analysis of GMO Foods

Screening for Genetically Modified Organisms in Food Using Promega’s Wizard® Resin

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Gene Expression & Reporters

Review: David C. Montefiori (2009) Measuring HIV Neutralization in a Luciferase Reporter Gene Assay. HIV Protocols, Second Edition, Vol. 485, V.R. Prasad and G.V. Kalpana, eds.

HaloTag® Technology: Convenient, Simple and Reliable Labeling from Single Wells to High-Content Screens

Expression of Fusion Proteins: How to Get Started with the HaloTag® Technology

Achieve the Protein Expression Level You Need with the Mammalian HaloTag® 7 Flexi® Vectors

Measuring Cell Health and Viability Sequentially by Same-Well Multiplexing Using the GloMax®-Multi Detection System

GloMax®-Multi Detection System: Luminescence, Fluorescence and Absorbance in One Instrument


Using the GloMax®-Multi Detection System for Measuring Signal from Multiplexed Fluorescent and Luminescent Assays

Luciferase Reporter Assays: Powerful, Adaptable Tools for Cell Biology Research

Bioluminescent Reporter Genes

Improved Response Ratio for NF-κB Inhibition Analysis Using New Luciferase Reporter Vector (pGL4.32[Luc2P/NF-κB-RE/Hygro])

ONE-Glo™ Luciferase Assay System: New Substrate, Better Reagent


The Bioluminescence Advantage

Deciphering the pGL4 Vector Code

New High-Throughput Technology for Screening Transcriptional Regulatory Elements and Gene Networks

HaloTag® Protein: A Novel Reporter Protein for Human Neural Stem Cells

Using the Beta-Glo® Assay System to Demonstrate Transport of β-Galactosidase Across the Blood-Brain Barrier


Introducing the Rapid Response™ Reporter Vectors

Introducing Chroma-Luc™ Technology

Increased Renilla Luciferase Sensitivity in the Dual-Luciferase® Reporter Assay System

Monster Green™ Protein: A Brighter, Longer-Expressing Green Fluorescent Protein

Introducing the Beta-Glo™ Assay System


Enhanced Response Dynamics for Transcription Analysis Using New pGL4 Luciferase Reporter Vectors

Normalizing Genetic Reporter Assays: Approaches and Considerations for Increasing Consistency and Statistical Significance

PIASy Regulation of STAT5 Activity: Different Reporter Vectors, Different Responses

Sensitive Detection of Reporter Gene Assay Results

Sensitive Homogeneous CEDIA® Analysis Using the Beta-Glo® Assay System

Using Luciferase Reporter Assays to Screen for GPCR Modulators


Quantitating Protein in Promega Lysis Buffers and Steady-Glo® and Bright-Glo™ Luciferase Reagents

Protein Determination in Promega Cell Lysis Buffers Using a Noninterfering Protein Assay

Choosing the Right Luciferase to Light Your Way

Beta-Glo® Assay System: A Luminescent β-Galactosidase Assay for Multiple Cell Types and Media

Dual-Glo™ Luciferase Assay System: A Homogeneous Dual-Reporter System


Dual-Glo™ Luciferase Assay System: Convenient Dual Reporter Measurements in 96- and 384-Well Plates

New Synthetic Renilla Gene and Assay System Increase Expression, Reliability and Sensitivity

Bright-Glo™ and Steady-Glo™ Luciferase Assay Systems: Reagents for Academic and Industrial Applications

Design of Homogeneous Luciferase Assays

A Steady-Glo™ Luciferase Assay System for High-Throughput Screening Applications


Technically Speaking: Eukaryotic Expression: Transfection and Reporter Vectors

Rapid Luciferase Reporter Assay Systems for High Throughput Studies

The Chemistry of Bioluminescent Reporter Assays

Alpha-Melanocyte-Stimulating Hormone Induction of Luciferase Expression under the Control of Human Tyrosinase Promoter/Enhancer

Compatibility of the CellTiter 96® AQueous One Solution with the Luciferase Assay System


Dual-Luciferase™ Reporter Assay: An Advanced Co-Reporter Technology Integrating Firefly and Renilla Luciferase Assays

CAT Reporter Systems: New pCAT®3 Reporter Vectors and Antibodies Provide Increased Expression and Detection Capabilities

Luciferase Assay System Vendor Comparison

Increased Expression and Convenience with the pGL3 Luciferase Reporter Vectors

Firefly Luciferase Engineered for Improved Genetic Reporting


Video Imaging of Regulated Firefly Luciferase Activity in Transgenic Plants and Drosophila

Firefly Luciferase as a Reporter for Plant Gene Expression Studies

A New Lysis Buffer for Luciferase, CAT and β-galactosidase Reporter Gene Co-Transfections


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Genetic Identity

Advances in DNA Typing: What is DNA Typing?

New Approaches to DNA Fingerprint Analysis

For more Genetic Identity Articles visit Profiles in DNA


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Genotype Analysis

Doing Good Science: Authenticating Cell Line Identity

Reviewed: The Challenge of Using SNPs in the Understanding and Treatment of Disease

ATCC Verifies Cell Line Identity Using Promega Reagents

Use of the READIT® SNP Genotyping System to Screen Mutations Generated Using the GeneEditor™ in vitro Site-Directed Mutagenesis System

READIT® SNP Genotyping System: Accurate, Flexible and Easy

The Y Chromosome Deletion Detection System, Version 1.1



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Imaging

In Vivo Evaluation of Regulatory Sequences by Analysis of Luciferase Expression

HaloTag® Technology: Convenient, Simple and Reliable Labeling from Single Wells to High-Content Screens

Cell Surface HaloTag® Technology: Spatial Separation and Bidirectional Trafficking of Proteins

Bioluminescence Imaging of Live Trout for Virus Detection using EnduRen™ Live Cell Substrate

Innovations in Fluorescence Imaging of Live Cells

HaloTag® Technology: Cell Imaging and Protein Analysis


Perform Multicolor Live- and Fixed-Cell Imaging Applications with the HaloTag® Interchangeable Labeling Technology

HaloTag® Interchangeable Labeling Technology for Cell Imaging and Protein Capture

Transfecting a Human Neuroblastoma Cell Line with Monster Green™ Fluorescent Protein

Using Promega Antibodies for Neuroscience Research

Immunohistochemical Staining Using Promega Anti-ACTIVE® and Apoptosis Antibodies


Specific Labeling of Neurons and Glia in Mixed Cerebrocortical Cultures


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Immunological Detection

Neuroprotection: Understanding the Modus Operandum of Glutamate Excitotoxicity

In the Literature: Anti β-galactosidase mAb

ELISA In Situ: A Sensitive Tool for Detecting Release of Endogenous BDNF

Immunostaining with the New Anti-ACTIVE® p38 Antibody

AttoPhos® AP Fluorescent Substrate and Western Blue® Stabilized Substrate Detect Cytoplasmic Alkaline Phosphatase in NIH3T3 Cells


Specific Labeling of Neurons and Glia in Mixed Cerebrocortical Cultures

Development of a Rapid Capture ELISA Using PCR Products and the PinPoint™ System

Technically Speaking: TGFβ1 ELISA System

Technically Speaking: EGGstract® IgY Purification System

Immunohistochemistry Using Anti-TGFβ1 Polyclonal Antibody


LPS-Stimulated MCAF Production by Bovine Parenchyma In Vitro

TGFβ1 ELISA System: A Unique Format That Adds Value and Flexibility

Development of Antibodies Reactive with the gamma-c (Common gamma) Chain of the Human Hematopoietic Receptor Superfamily

Transforming Growth Factor β1 (TGFβ1): A Biological Paradox

Control and Modulation of Neonatal Myocyte Calcium Movements by Cytokines


Why IgY? Chicken Polyclonal Antibody, An Appealing Alternative

Immunocytochemistry for Specific Growth Factors

The Chemokine Network: Coordinate Mediators of Early Inflammatory Events

Stabilized TMB Substrate for Horseradish Peroxidase vs. 4-chloro-1-naphthol: A Comparison on Western Blots

Western Blotting Using the ProtoBlot® II AP System with Stabilized Substrate


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Interviews and Thoughts on Science

Editor's Pick: The Future is Bright: Imaging with Luciferase in Whole Animals

Pharmaceutical Research and Development in India


Screening for New Chemical Entities in Academic Settings

High-Througput Screening and Drug Discovery at St. Jude Children's Research Hospital

Bird Flu: The Next Pandemic?

A New Paradigm for Life Science Research (Interview with Jeremy Caldwell, Ph.D.)

Luciferase: From Bugs to Biotech and Beyond


Capitalizing on China-Based Research and Development

NIH Forms Network of Molecular Screening Centers to Identify Novel Chemical Probes for Life Science Research

Monsters or Research Tools? The Use of Chimeras in Biomedical Research

Getting More Personalized Through Technology

Peepers and Creekers and Snorers, Oh My!


Scientists and Entropy: Lessons from Literature

The Biological Basis of Absolute Pitch

Prions: The Unfolding Story of a Good Protein Gone Bad, Part I: Introduction to Prion Diseases and Biology

Prions: The Unfolding Story of a Good Protein Gone Bad, Part II: Neuroinvasion and Replication

Prions: The Unfolding Story of a Good Protein Gone Bad, Part III Research: Transmission and Diagnostics


The Last Great Invention of the 20th Century

The Presenilins: A Review

Molecular Techniques and Conservation Biology

Recent Developments in Longevity (Today Could Be The Day series)

Recent Developments in Breast Cancer (Today Could Be The Day series)


Recent Developments in HIV (Today Could Be The Day series)

A Stinging Issue: Declining Pollinator Populations

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In Vitro Transcription

A New System for Cloning and Expressing Protein-Coding Regions

Purification of In Vitro Transcribed RNA Using the SV Total RNA Isolation System

Fast & Efficient Production of RNA with the T7 RiboMAX™ Express System

Use of MagneHis™ Particles and His-Tagged RNA Polymerase to Study Stalled Elongation Complexes at DNA Lesions 
From the article Jung, Y. and Lippard, S.J. (2003) J. Biol. Chem. 278, 52084–92.

T7 RiboMAX™ Express: Generation of 27kb in vitro Transcripts in Minutes


Characterization of TNT® T7 Quick for PCR DNA

Using ImProm-II™ Reverse Transcription System to Generate Fluorescent Probes

A Simple and Efficient Method to Produce RNAs with Homogenous 3´ Ends

Ribo m7G Cap Analog: A Reagent for Preparing In Vitro Capped Transcripts

In Vitro Transcription on DNA Templates Immobilized to Streptavidin MagneSphere® Paramagnetic Particles


A Comparative Study of T7 RNA Polymerase Quality

Vendor Comparison: in vitro Transcription Systems

In Vitro Transcription from Biotinylated DNA Immobilized on Streptavidin MagneSphere® Paramagnetic Particles

cDNA Synthesis Using the Universal RiboClone® System

DNA Excision Repair Catalyzed in vitro by the HeLaScribe® Nuclear Extract


Technically Speaking: Riboprobe® and RiboMAX™ in vitro Transcription Systems

Technically Speaking: RiboClone™ cDNA Synthesis Systems and Related Cloning Products

Production of Milligram Amounts of Highly Translatable RNA Using the RiboMAX™ System

Transcriptional Activation Assays of Recombinant Transcription Factors


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Nucleic Acid Microarrays

Synthesizing Fluorescent Target cDNA for Microarray-Based Transcriptional Profiling: Considerations Regarding Primers and Template RNA

The ChipShot™ Membrane Clean-Up System: A Fast Simple Technique for Efficient Recovery of Highly Purified cDNA

Pronto!™ Plus Systems: New Integrated Reagent Systems for Optimal Microarray Performance

The Pronto!™ Plus System: Increasing Microarray Reproducibility, Reliability and Ease of Use

Indirect Fluorescent Labeling of Microarray Targets Using ImProm-II™ Reverse Transcriptase


MagneSil™ Paramagnetic Particles: A High-Throughput PCR Purification System for Microarrays

Technically Speaking: Microarrays

Microarray Applications Using Promega Products

Using ImProm-II™ Reverse Transcription System to Generate Fluorescently Labeled Probes

Isolating High-Quality mRNA From Total RNA with the PolyATtract® mRNA Isolation System For Use in Expression Profiling with the Pronto!™ Plus System


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Mutagenesis

Options for the Protein Truncation Test Protocol: A Molecular Diagnostics Application

Technically Speaking: GeneEditor™ in vitro Site-Directed Mutagenesis System

The Protein Truncation Test (PTT)

The GeneEditor™ in vitro Mutagenesis System: Site-Directed Mutagenesis Using Altered Beta-Lactamase Specificity

Site-Directed Mutagenesis



Altered Sites® II in vitro Mutagenesis Systems

Mutagenesis and Mammalian Expression Using the Altered Sites® II Mammalian Mutagenesis System

Generation of Nested Deletions using the Erase-A-Base® System

Altered Sites® II In Vitro Mutagenesis Systems

Site-directed Mutagenesis Using the Altered Sites® II Systems



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PCR

Introducing GoTaq® qPCR Master Mix: The Bright Choice for Dye-Based qPCR

Ensuring Successful PCR Using Online Resources

Get the Convenience of Hot-Start PCR with the New GoTaq® Hot Start Polymerase

GoTaq® Hot Start Polymerase for Mouse Genotyping

GoTaq® Hot Start Polymerase Successfully Amplified Allele C of Polymorphism 311 (Cys→Ser) on the PON2 Gene

The Plexor™ Systems Provide Accurate Quantitation in Multiplex qPCR and qRT-PCR


Activity of Promega Restriction Enzymes in GoTaq® Green Master Mix and PCR Master Mix

GoTaq® Green Master Mix: From Amplification to Analysis

Plexor™ Technology: A New Chemistry for Real-Time PCR

Analyses of Gene Disruption by Whole-Cell PCR Using the GoTaq® Green Master Mix

GoTaq® Flexi DNA Polymerase: Robust Performance with Magnesium Optimization


Quantitative, Real-Time RT-PCR Expression Using the SV 96 Total RNA Isolation System

Introducing GoTaq™ DNA Polymerase: Improved Amplification With a Choice of Buffers

Wizard® SV Gel and PCR Clean-Up System

Introducing the Wizard® SV 96 PCR Clean-Up System

Compatibility of GoTaq™ DNA Polymerase-Amplified PCR Products with TnT® T7 Quick for PCR DNA


An Integrated Approach to Studying Apoptosis: From Gene Expression to Cellular Events

Automated Purification of PCR Products Using MagneSil™ Paramagnetic Particles

Performance Advantages Designed into Promega's PCR Master Mix (Promega Notes 2001)

Performance Advantages Designed into Promega's PCR Master Mix (eNotes 2001)

Characterization of TNT® T7 Quick for PCR DNA


Long PCR Amplification of p300 cDNA Using a Blend of Taq DNA Polymerase and Proofreading Pfu DNA Polymerase

A New TNT® System for Enhanced Expression of PCR DNA

Rapid Partial Digestion of DNA Fragments by PCR with 5-methyl-dCTP

Screening of the Gamµ-7 Microsatellite Locus to Determine the Sex of Captive Whooping Cranes

Extraction and Amplification of DNA from an Ancient Moss


Pfu DNA Polymerase: A High Fidelity Enzyme for Nucleic Acid Amplification

Primer Pairs for Mediator and Receptor mRNA Analysis

Betaine and DMSO: Enhancing Agents for PCR

Primer Pairs for Neurotrophic Factor mRNA Analysis

PCR Core Systems: Complete Reagent Systems for DNA Amplification


Technically Speaking: DNA Amplification: Preparation of Template and Recovery of Product

Amplification of Flanking Regions: New Applications and Performance Optimization of Single Specific Primer PCR and T-Vector Cloning

Improved PCR Amplification Using TaqBead™ Hot Start Polymerase

Digestion of PCR and RT-PCR Products with Restriction Endonucleases Without Prior Purification or Precipitation

Large-Scale Gel Purification of DNA Fragments Using Wizard® PCR Preps Resin


Direct Sequencing of PCR Products with Degenerate Primers


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Plant Biotechnology

Expanding the Capabilities of Plant Genomic DNA Purification

Automating the Wizard® Magnetic 96 DNA Plant System on Thermo Labsystems' Kingfisher®

Isolation of Genomic DNA from Agricultural Bacteria Using the Wizard® MagneSil™ Plasmid Purification System

Automated Genomic DNA Purification Using the Wizard® Magnetic 96 DNA Plant System

Wizard® Genomic DNA Purification Kit and the Isolation of Plant Genomic DNA


Titration of Tomato Leaf Material Used as Starting Material for SV Total RNA Isolation

Automated Isolation of Genomic DNA Using Promega’s Wizard® SV 96 DNA Binding Plates on the Beckman Biomek® 2000

Automated Poly(A)+ mRNA Isolation From Plant Tissues Using the PolyATtract® System 1000


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Protein Interactions & Microarrays

Protein Micorarrays

Multiplexed Protein-Interaction Studies Using HaloLink™ Protein Arrays



Protein Interactions

Expression of Fusion Proteins: How to Get Started with the HaloTag® Technology

Achieve the Protein Expression Level You Need with the Mammalian HaloTag® 7 Flexi® Vectors

ProTEV Protease for Fusion Protein Processing

HaloCHIP™ System: Mapping Intracellular Protein:DNA Interactions using HaloTag® Technology

Compatibility of Single Step (KRX) Competent Cells with the MagneGST™ Pull-Down System



Detecting DNA-Binding Protein Activity Using a Membrane-Based Approach

HaloTag™ Interchangeable Labeling Technology for Cell Imaging, Protein Capture and Immobilization

In Vitro His-Tag Pull-Down Assay Using MagZ™ Particles

Detection of Protein:Protein Interactions Using the MagneGST™ Pull-Down System

Applications of Promega's In Vitro Expression Systems



A General Method for Isolating Targets of RNA and DNA Binding Proteins

The CheckMate™ Mammalian Two-Hybrid System

The TNT® T7 Quick Coupled Transcription/Translation System

Application of Promega's TNT® T7 Coupled Reticulocyte Lysate System in the Study of Fas-Associated Apoptosis

Applications of the TNT® Coupled Transcription/Translation Systems



Gel Shift Assay System

Applications of TNT® Coupled Transcription/Translation Systems

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Protein Expression and Analysis

Cell-Free Expression

TNT® T7 Quick Coupled Transcription/Translation System—Quick Screening of Protein Expression Using Common T7-Promoter-Containing Vectors

A Guide to Optimizing Protein Synthesis in the S30 T7 High-Yield Protein Expression System

Expressing Mammalian Proteins Using Insect and Rabbit Cell-Free Lysates

Cell-Free Protein Expression with the TNT® T7 Insect Cell Extract Protein Expression System

The S30 T7 High-Yield Protein Expression System



Selecting the Cell-Free Protein Expression System that Meets Your Experimental Goals

TNT® SP6 High-Yield Protein Expression System: More Protein from a Coupled Transcription/Translation System

Expression of Soluble Native Human Proteins in Cell-Free Extracts

Wheat Germ Extract Plus For Higher Protein Yields

Technically Speaking: TNT® Rabbit Reticulocyte Lysate Systems — Easy Protein Expression



Optimize Your TNT® Reticulocyte Lysate Systems Reaction

Express More Functional Protein: TNT® Quick Coupled Transcription/Translation Systems

Compatibility of GoTaq™ DNA Polymerase-Amplified PCR Products with TNT® T7 Quick for PCR DNA

Optimized Gene Expression with the T7 Sample System

Characterization of TNT® T7 Quick for PCR DNA



Characterization of TNT® T7 Quick for PCR DNA

A New TNT® System for Enhanced Expression of PCR DNA

Applications of Promega's In Vitro Expression Systems

In vitro Expression Cloning Using the TNT® Coupled Reticulocyte Lysate System

Applications of the TNT® T7 Quick Coupled Transcription/Translation System



The TNT® T7 Quick Coupled Transcription/Translation System

Application of Promega's TNT® T7 Coupled Reticulocyte Lysate System in the Study of Fas-Associated Apoptosis

TNT® Lysate Coupled Transcription/Translation: Comparison of the T3, T7 and SP6 Systems

Post-Translational Processing: Use of the TNT® Lysate Systems with Canine Microsomal Membranes

 

 



Cell-Based Expression

KRX Autoinduction Protocol: A Convenient Method for Protein Expression

Protein Expression in Less Time: A Short Induction Protocol for KRX

A Novel System for Regulated Protein Expression in Mammalian Cells

Clone and Express Protein-Coding Regions using the Flexi® Vector Systems



pTARGET™ Vector: A New Mammalian Expression TVector

The pCI-neo Mammalian Expression Vector

pCI and pSI Mammalian Expression Vectors



Protein Purification

Compatibility of Single Step (KRX) Competent Cells with the MagneGST™ Pull-Down System

Maxwell® 16 Polyhistidine Protein Purification Kit: Automated Protein Purification with Maximum Performance and Convenience

Metal Affinity Tag for Protein Expression and Purification using the Flexi® Vectors

Automated High-Throughput Protein Purification with MagneHis™ Particles

Purification of Recombinant Polyhistidine-Tagged Proteins From Mammalian Cells Using the MagneHis™ Protein Purification System



HisLink™ 96 Protein Purification System: Fast Purification of Polyhistidine-Tagged Proteins

The MagZ™ System: His-Tagged Protein Purification without Hemoglobin Contamination

Efficient Purification of His-Tagged Proteins From Insect and Mammalian Cells

Purification of GST-Fusion Proteins by Magnetic Resin-Based MagneGST™ Particles

Introducing the HisLink™ Protein Purification Resin



Automated Polyhistidine-Tagged Protein Purification Using the MagneHis™ Protein Purification System

A One-Step, Nondenaturing Purification Method for Recombinant Proteins Produced in E. coli



Protein Labeling and Analysis

Conveniently perform in-solution digestions with immobilized trypsin

HaloTag® Technology: Convenient, Simple and Reliable Labeling from Single Wells to High-Content Screens

Improve Protein Analysis with the New, Mass Spectrometry-Compatible ProteasMAX™ Surfactant

Use of Anti-β-Galactosidase Antibody to Identify Transplanted Bone Marrow-Derived Cells Expressing Bacterial β-Galactosidase

[U-13C, 15N] Protein Labeling Using Escherichia coli Strain KRX

Non-Radioactive Identification of Protein:Protein Interactions Using TNT® SP6 High-Yield Protein Expression System



ProTEV Protease for Fusion Protein Processing

15N Labeling of Proteins Overexpressed in the Escherichia coli Strain KRX

Compatibility of Single Step (KRX) Competent Cells with the MagneGST™ Pull-Down System

Selenomethionine Protein Labeling Using the Escherichia coli Strain KRX

Alternative Elution Conditions for the Mass Spectrometry Analysis of Polyhistidine-Tagged Proteins



A Polyclonal HaloTag® Antibody for Western Blotting and Immunocytochemistry

HaloTag® Protein: A Novel Reporter Protein for Human Neural Stem Cells

Dephosphorylation of Proteins with Shrimp Alkaline Phosphatase

Detecting DNA-Binding Protein Activity Using a Membrane-Based Approach

HaloLink™ Resin: High Capacity, Specificity and Scalable Throughput for Protein Analysis



Clone and Express Protien-Coding Regions Using the Flexi® Vector Systems

HaloTag™ Interchangeable Labeling Technology for Cell Imaging, Protein Capture and Immobilization

In Vitro His-Tag Pull-Down Assay Using MagZ™ Particles

Detection of Protein:Protein Interactions Using the MagneGST™ Pull-Down System

AttoPhos® AP Fluorescent Substrate and Western Blue® Stabilized Substrate Detect Cytoplasmic Alkaline Phosphatase in NIH3T3 Cells



FluoroTect™ GreenLys in vitro Translation Labeling System

Mass Spectrometric Analysis of MagneHis™ Purified Proteins

Compatibility of the Pierce BCA Protein Assay with Promega Lysis Buffers and Lytic Assay Reagents

Applications of Promega's In Vitro Expression Systems

Helpful Hints for Analyzing in vitro Transcription/Translation Reactions on Polyacrylamide Gels



The Protein Truncation Test (PTT)

The ISOQUANT™ Protein Deamidation Detection Kit

Detection of RNA Polymerase II in Mammalian Species by Western Blot Analysis

Post-Translational Processing: Use of the TNT® Lysate Systems with Canine Microsomal Membranes

Improved Staining of Small PVDF-Immobilized Proteins

Selection of Protein Stains for SDS Gel Electrophoresis

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RT-PCR & cDNA Synthesis

Detecting PMCA Transcripts in Single Hippocampal Neurons


Detecting Pituitary Adenylate Cyclase-Activating Polypeptide in Cartilaginous Fish Brain

The Plexor™ Systems Provide Accurate Quantitation in Multiplex qPCR and qRT-PCR

GoTaq® Green Master Mix for Quick and Easy Two-Step RT-PCR

RNasin® Ribonuclease Inhibitors: Superior Performance for All of Your RNA Analysis Needs

RNasin® Plus RNase Inhibitor: New Protein for High Temperature RNase Inhibition



Quantitative, Real-Time RT-PCR Expression Using the SV 96 Total RNA Isolation System

Using ImProm-II™ Reverse Transcriptase to Construct cDNA Libraries

Using ImProm-II™ Reverse Transcription System for Two-Step RT-PCR

Using ImProm-II™ Reverse Transcription System for Coupled RT-PCR

Technically Speaking: Promega RT-PCR Systems Explained

Using ImProm-II™ Reverse Transcription System to Generate Fluorescently Labeled Probes



Cloning Differential Display-PCR Products with pGEM®-T Easy Vector System

Detection of Enteric Pathogenic Viruses in Shellfish by RT-PCR

Single-Cell RT-PCR using RNasin® Ribonuclease Inhibitor

Use of M-MLV RT, RNase H-, Point Mutant, for mRNA-Differential Display Analysis of Parathyroid Hormone-Related Peptide (PTHrP)-Treated Breast Carcinoma Cells

Single-Cell RT-PCR of Laser Capture-Microdissected Purkinje Cells



Technically Speaking: M-MLV Reverse Transcriptase, RNase H Minus

Using the Access RT-PCR System: Reaction Parameters that Affect Efficient Amplification

Measuring Gene Expression from Mammalian Brain Tissue

Using the Access RT-PCR System: Reaction Parameters That Affect Efficient Amplification

Quantitative RT-PCR: Rapid Construction of Templates for Competitive Amplification

Rapid Isolation of RNA from Small Quantities of Human Whole Blood for use in RT-PCR Analysis



Digestion of PCR and RT-PCR Products With Restriction Endonucleases Without Prior Purification or Precipitation

Technically Speaking: Access RT-PCR System

cDNA Synthesis using the Universal RiboClone® System

A Rapid Protocol for the Isolation of Total RNA Suitable for RT-PCR

PCR ACCESS! A Sensitive Single-tube, Two-enzyme System for RT-PCR



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RNA Interference

MicroRNA Biosensors: Application for the psiCHECK™-2 Vector

RNAi Knockdown in Primary Cells: Suppression of Target Gene with psiSTRIKE™ and psiCHECK™ Vectors and amaxa's Nucleofector® Technology

microRNA Biosensors: Application for the psiCHECK™-2 Vector

Human RNAi Promoters Work Effectively in Mouse NIH/3T3 Cells

Using Bioluminescent Reporter Genes to Optimize shRNA Target Sites for RNAi of the bcr/abl Gene


RNA Interference in Drosophila S2 Cells: Effect of dsRNA Size, Concentration and Exposure Time

GeneClip™ U1 Hairpin Cloning Systems for Expression of Short Hairpin RNAs in vivo

Suppression of Caspase-3 Expression Using the psiSTRIKE™ hMGFP Vector

The Use of Bioluminescent Reporter Genes for RNAi Optimization

Produce Functional siRNAs and Hairpin siRNAs using the T7 RiboMAX™ Express RNAi System


The T7 RiboMAX™ Express RNAi System: Efficient Synthesis of dsRNA for RNA Interference

RNAi: Shutting Down the Messenger

Citation Note: ProInflammatory Phenotype of Coronary Arteries Promotes Endothelial Apoptosis in Aging

Knocking Down p53 Expression Using RNA Interference

RNAi in Drosophila S2 Cells: Effect of dsRNA Size, Concentration, and Exposure Time

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RNA Purification and Analysis

RNA Purification Kit Comparison: Yield, Quality and Real-Time RT-PCR Performance

Perform Automated Nucleic Acid Purification Using the SV Systems and the epMotion® 5075 VAC Workstation

Maxwell® 16 Tissue and Cell Total RNA LEV Systems

PureYield™ RNA Midiprep System: Isolating Pure Total RNA without DNase

Purification of In Vitro Transcribed RNA Using the SV Total RNA Isolation System


MagneSil® Total RNA mini-Isolation System

Quantitative, Real-Time RT-PCR Expression Using the SV 96 Total RNA Isolation System

Promega's SV Membrane Technology: The Evolution of an Indispensable Laboratory Tool

High-Throughput Purification Using the SV 96 Total RNA Isolation System

RNasin® Ribonuclease Inhibitor Part I: Characterization of the Protein


RNasin® Ribonuclease Inhibitor Part II: A Tale of Two Proteins

Isolation and Analysis of Nucleic Acids from Human Blood: A Molecular Diagnostics Application

Automated Poly(A)+ mRNA Isolation From Plant Tissues Using the PolyATtract® System 1000

A Simple and Efficient Method to Produce RNAs with Homogenous 3´ Ends

Titration of Tomato Leaf Material Used as Starting Material for SV Total RNA Isolation


Measuring Gene Expression from Mammalian Brain Tissue

Can the SV RNA Isolation System be Used to Purify RNA from Frozen, Unprocessed Whole Blood?

Isolation of RNA from Pacific Oyster Larvae Using the SV Total RNA Isolation System

Isolating Nucleic Acids from Small Amounts of Brain Lysates Using the SV Total RNA Isolation System

Separate Isolation of Genomic DNA and Total RNA from Single Samples Using the SV Total RNA Isolation System


Molecular Diagnostics: Isolation and Analysis of RNA from Human Blood

Isolation of RNA from Plant, Yeast and Bacteria

Technically Speaking: SV Total RNA Isolation System

RNA Purification: A Rapid and Versatile Protocol for the Isolation of Total RNA


Technically Speaking: Tips for Working with RNA and Troubleshooting Downstream Applications

Rapid Isolation of RNA from Small Quantities of Human Whole Blood for use in RT-PCR Analysis

PolyATtract® Systems for mRNA Purification

Technically Speaking: PolyATtract® mRNA Isolation Systems

Isolation of Total RNA and mRNA from Plant Tissues


Use of a 96 Well Format for Small Scale mRNA Isolation and cDNA Synthesis

RNase Protection Assay System: A Versatile Technique for the Analysis of RNA

Staining Nucleic Acids with Silver: An Alternative to Radioisotopic and Fluorescent Labeling

Isolation of mRNA from Plant Tissues Using the PolyATtract® System 1000

Rapid, High Yield Isolation of Yeast mRNA


30-Minute poly(A)+ RNA Isolation Directly from Tissue

RNase ONE™: Advantages for Nuclease Protection Assays

Magnetic Isolation of mRNA from Small Samples


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Sequencing

Wizard® SV 96 Plasmid DNA Purification System: High Quality Plasmid DNA for Use in Fluorescent Sequencing Methods

T4 RNA Ligase: A Molecular Tool for RNA and DNA Manipulations

SILVER SEQUENCE™ DNA Sequencing System Duplicating Films

Effects of Template Preparation on the SILVER SEQUENCE™ DNA Sequencing System

Rapid PCR Sequencing of Plasmid DNA Directly from Colonies of Saccharomyces cerevisiae



SILVER SEQUENCE™ DNA Sequencing System

Direct Sequencing of PCR Products with Degenerate Primers



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Transfection

Getting the Most from Your Transfections: Increasing Throughput and Sensitivity

Transfecting a Human Neuroblastoma Cell Line with Monster Green™ Fluorescent Protein

Wizard MagneSil Tfx™ System for the Purification of Transfection-Grade DNA

TransFast™ Transfection Reagent Update

Technically Speaking Eukaryotic Expression: Transfection and Reporter Vectors


An Efficient New Transfection Reagent for Eukaryotic Cells: TransFast™ Transfection Reagent

Tfx™-50 Reagent and High Efficiency Transfection of Vascular Tissue in vitro and in vivo

Transfection of Insect Cells with Tfx™-20 Reagent

Tfx™-50 Reagent Increases the Uptake of Oligonucleotides by Leukemic Cells

Tfx™-50 Reagent: A New Transfection Reagent For Eukaryotic Cells


Increased Gene Expression in Mammalian Cell Lines using pAdVAntage™ DNA as a Co-Transfectant

A New Lysis Buffer for Luciferase, CAT and β-Galactosidase Reporter Gene Co-Transfections

Optimization of Transfectam®-Mediated Transfection Using a Luciferase Reporter System