ProteaseMax™ Surfactant, Trypsin Enhancer

ProteaseMax™ Surfactant, Trypsin Enhancer is designed to enhance the performance of trypsin or chymotrypsin in any digestion reaction performed with the objective of characterizing the products by mass spectrometry or liquid chromatography.

Improved recovery from gels

Increase protein sequence coverage increasing confidence of correct protein identification.

Improved protein digestion

Enable cleavage at sites that were not accessible due to secondary structure.
Reduce standard digestion time from overnight to 3 hours.

Self-degradable

Use directly for mass spec analysis without additional inactivation steps such as heating or acid treatment.

TNT® Quick Coupled Transcription/Translation System

The TNT Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression. To use these systems, 0.2–2.0μg of circular plasmid DNA containing a T7, T3 or SP6 promoter, or a PCR generated fragment containing a T7 promoter, is added to an aliquot of the TNT Quick Master Mix and incubated in a 50μl reaction volume for 60 minutes at 30°C.

Obtain data faster

Functional protein is expressed in only one hour, not days as with cell based expression systems.

Consistent reliable results

This mammalian based system expresses soluble, functional proteins that are post-translationally modified, unlike E. coli based systems.

Few steps

Expressed proteins can be used directly after expression, no requirement for additional purification.