We offer a complete range of firefly and Renilla luciferase reporter vectors for promoter analysis, nuclear receptor studies, protein interaction analysis and signaling pathway studies. We also offer CAT, Beta-Galactosidase, and GFP control vectors.
pGL4 Luciferase Reporter Vectors
Optimized luciferase reporter vectors with both firefly and Renilla luciferases in a variety of configurations. Now also available with response elements to study pathways of interest.
miRNA Target Expression Vector
The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene (luc2).
pRL Renilla Luciferase Control Vectors
Provide constitutive expression of Renilla luciferase. Expression of Renilla luciferase provides an internal control value to which expression of the experimental firefly luciferase reporter gene may be normalized.
Beta-Galactosidase Control Vector
Positive control vector for monitoring transfection efficiencies of mammalian cells. Co-transfection with firefly luciferase vectors provides cell extracts that can be assayed for both luciferase and β-galactosidase activities.
This animation introduces the concept of reporter gene assays, using the Dual-Luciferase® Assay as a model.
This chapter of the Protocols and Applications Guide discusses the use of bioluminescent reporters to study eukarytoic gene expression and regulation including the use of single-reporter assays, dual-reporter assays, and live-cell substrates.