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Promega Corporation

pmirGLO Dual-Luciferase miRNA Target Expression Vector

The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene (luc2). Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with firefly luciferase (luc2) used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization and selection.

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pmirGLO Dual-Luciferase miRNA Target Expression Vector

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pmirGLO Dual-Luciferase miRNA Target Expression Vector

 Components

  • Oligo Annealing Buffer

    C838A1 x 1ml
  • pmirGLO Vector

    E133A1 x 20μg
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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20μg
- E1330 $ 360.00 Add to cart


GenBank®/EMBL Accession Number

pmirGLO Vector Accession Number FJ376737; pmirGLO Vector sequence text file.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

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