Reporter Vectors and Cell Lines

pmirGLO Dual-Luciferase miRNA Target Expression Vector

Quantitatively measure microRNA activity with a luciferase reporter. The firefly luciferase (luc2) vector is the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acts as a control reporter for normalization and selection.

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pGL4 Luciferase Reporter Vectors

These next generation, luciferase reporter vectors offer more efficient expression of both firefly and Renilla luciferases in mammalian cells. Use the pGL4 Vector Selector to find the ideal vector for your application.

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GloResponse™ Luciferase Reporter Cell Lines

Experience state-of-the-art luciferase reporter technology integrated into a cell line. Develop a reporter assay based on the pathway of interest regulating the luciferase gene.

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• Technical Article

pGL3 and pGL2 Luciferase Reporter Vectors

Find tools for the quantitative analysis of cis- or trans-factors regulating mammalian gene expression. Redesigned vectors increase expression and contain a modifed luciferase region.

• pGL3 Vector Information
• pGL2 Vector Information

This chapter of the Protocols and Applications Guide discusses the use of bioluminescent reporters to study eukarytoic gene expression and regulation including the use of single-reporter assays, dual-reporter assays, and live-cell substrates.

This animation introduces the concept of reporter gene assays, using the Dual-Luciferase® Assay as a model.