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Promega Corporation

β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

The β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer is a convenient method for assaying β-galactosidase activity in lysates prepared from cells transfected with β-galactosidase reporter vectors such as the pSV-β-Galactosidase Control Vector.

The standard assay is performed by adding a dilute sample to an equal volume of Assay 2X Buffer that contains the substrate ONPG (o-nitrophenyl-β-d-galactopyranoside). Samples are incubated for at least 30 minutes, during which time the β-Galactosidase hydrolyzes the colorless substrate to o-nitrophenyl, which is yellow. The reaction may be terminated by addi...

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β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

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β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

 Components

  • Sodium Carbonate

    E202A1 x 35ml
  • Assay 2X Buffer

    E203A1 x 10ml
  • Reporter Lysis 5X Buffer

    E397A1 x 30ml
  • β-Galactosidase

    E801A1 x 100u
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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10ml
- E2000 $ 152.00 Add to cart


Available Separately

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Reporter Lysis 5X Buffer

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Reporter Lysis 5X Buffer

  Components

  • Reporter Lysis 5X Buffer

    E397A1 x 30ml
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  • This product can be added to a

  • Helix Cabinet

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.


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30ml
- E3971 $ 55.00 Add to cart

Storage Conditions

Reporter Lysis Buffer may be stored at room temperature. Store other system components at –20°C.

For product intended use please see Patents & Disclaimers tab.

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