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Promega Corporation

β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

The β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer is a convenient method for assaying β-galactosidase activity in lysates prepared from cells transfected with β-galactosidase reporter vectors such as the pSV-β-Galactosidase Control Vector.

The standard assay is performed by adding a dilute sample to an equal volume of Assay 2X Buffer that contains the substrate ONPG (o-nitrophenyl-β-d-galactopyranoside). Samples are incubated for at least 30 minutes, during which time the β-Galactosidase hydrolyzes the colorless substrate to o-nitrophenyl, which is yellow. The reaction may be terminated by addi...

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Product Size Conc. Catalog # *List Price QTY Add to Cart

β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

10ml
- E2000 $ 152.00 Add to cart


Available Separately

Product Size Conc. Catalog # *List Price QTY Add to Cart

Reporter Lysis 5X Buffer

30ml
- E3971 $ 55.00 Add to cart

Storage Conditions

Reporter Lysis Buffer may be stored at room temperature. Store other system components at –20°C.

For product intended use please see Patents & Disclaimers tab.

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