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Promega Corporation

CheckMate™/Flexi® Vector Mammalian Two-Hybrid System

The CheckMate™/Flexi® Vector Mammalian Two-Hybrid System provides a means to confirm, validate and study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays. Developed primarily for mammalian proteins of interest, the system can allow protein expression and post-translational modifications in an environment mimicking the native cell milieu. It is patterned on the yeast two-hybrid system with one protein of interest ("X") fused to a DNA-binding domain and the other protein ("Y") fused to a transcriptional activation domain (Figure 1).

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

CheckMate™/Flexi® Vector Mammalian Two-Hybrid System

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CheckMate™/Flexi® Vector Mammalian Two-Hybrid System

 Components

  • pFN10A (ACT) Flexi® Vector

    C933B1 x 5μg
  • pFN11A (BIND) Flexi® Vector

    C934B1 x 5μg
  • pGL4.31[luc2P/GAL4UAS/Hygro] Vector

    C935A1 x 20μg
  • pBIND Vector

    E245A1 x 20μg
  • pACT Vector

    E246A1 x 20μg
  • pBIND-Id Control Vector

    E247A1 x 20μg
  • pACT-MyoD Control Vector

    E248A1 x 20μg
1 each
- C9360 $ 748.00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

pFN10A (ACT) Flexi® Vector

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pFN10A (ACT) Flexi® Vector

 Components

  • pFN10A (ACT) Flexi® Vector

    C933A1 x 20μg
20μg
- C9331 $ 298.00 Add to cart

pFN11A (BIND) Flexi® Vector

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pFN11A (BIND) Flexi® Vector

 Components

  • pFN11A (BIND) Flexi® Vector

    C934A1 x 20μg
20μg
- C9341 $ 298.00 Add to cart

pGL4.31[luc2P/GAL4UAS/Hygro] Vector

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pGL4.31[luc2P/GAL4UAS/Hygro] Vector

 Components

  • pGL4.31[luc2P/GAL4UAS/Hygro] Vector

    C935A1 x 20μg
20μg
- C9351 $ 372.00 Add to cart

CheckMate™ Positive Control Vectors

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CheckMate™ Positive Control Vectors

 Components

  • pBIND-Id Control Vector

    E247A1 x 20μg
  • pACT-MyoD Control Vector

    E248A1 x 20μg
1 set
- C9370 $ 204.00 Add to cart

CheckMate™ Negative Control Vectors

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CheckMate™ Negative Control Vectors

 Components

  • pBIND Vector

    E245A1 x 20μg
  • pACT Vector

    E246A1 x 20μg
1 set
- C9380 $ 135.00 Add to cart

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GenBank®/EMBL Accession Number

pFN10A(ACT) Flexi® Vector Accession Number DQ487211; pFN10A(ACT) Flexi® Vector sequence text file.
pFN11A(BIND) Flexi® Vector Accession Number DQ487212; pFN11A(BIND) Flexi® Vector sequence text file.
pGL4.31[luc2P/GAL4UAS/Hygro] Accession Number DQ487213; pGL4.31[luc2P/GAL4UAS/Hygro] sequence text file.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

Representation of the CheckMate/Flexi Vector Mammalian Two-Hybrid System protocol.Figure 1. Representation of the CheckMate™/Flexi® Vector Mammalian Two-Hybrid System.

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pFN10A (ACT) Flexi Vector.Figure 2. pFN10A (ACT) Flexi® Vector.
pFN11A (BIND) Flexi Vector.Figure 3. pFN11A (BIND) Flexi® Vector.
pGL4.31[luc2P/GAL4UAS/Hygro] Vector.Figure 4. pGL4.31[luc2P/GAL4UAS/Hygro] Vector.
Cloning a protein-coding region into the Flexi Vectors.Figure 5. Cloning a protein-coding region into the Flexi® Vectors.

Use Restrictions

C9360, C9331, C9341, C9351, C9370, C9380 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

C9360, C9341, C9351, C9370, C9380 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

C9360, C9331, C9341 European Pat. No. 1685247 and other patents pending.

C9360, C9351 U.S. Pat. No. 5,670,356.

C9360, C9331, C9341 Patent Pending.

C9351 U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

C9351 U.S. Pat. No. 7,728,118.

C9360, C9331, C9341 For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

C9360, C9351 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

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