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Promega Corporation

CheckMate™/Flexi® Vector Mammalian Two-Hybrid System

The CheckMate™/Flexi® Vector Mammalian Two-Hybrid System provides a means to confirm, validate and study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays. Developed primarily for mammalian proteins of interest, the system can allow protein expression and post-translational modifications in an environment mimicking the native cell milieu. It is patterned on the yeast two-hybrid system with one protein of interest ("X") fused to a DNA-binding domain and the other protein ("Y") fused to a transcriptional activation domain (Figure 1).

The sy...

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  • Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart

CheckMate™/Flexi® Vector Mammalian Two-Hybrid System

1 each
- C9360 $ 809.00 Add to cart


Available Separately

Product Size Conc. Catalog # *List Price QTY Add to Cart

pFN10A (ACT) Flexi® Vector

20μg
- C9331 $ 322.00 Add to cart

pFN11A (BIND) Flexi® Vector

20μg
- C9341 $ 322.00 Add to cart

pGL4.31[luc2P/GAL4UAS/Hygro] Vector

20μg
- C9351 $ 402.00 Add to cart

CheckMate™ Positive Control Vectors

1 set
- C9370 $ 220.00 Add to cart

CheckMate™ Negative Control Vectors

1 set
- C9380 $ 146.00 Add to cart

Flexi® System, Entry/Transfer

5 entry and 20 transfer reactions
- C8640 $ 285.00 Add to cart

JM109 Competent Cells, >107cfu/μg

1ml
(5 × 200μl)
- L1001 $ 105.00 Add to cart

GenBank®/EMBL Accession Number

pGL4.31[luc2P/GAL4UAS/Hygro], DQ487213.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

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