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Promega Corporation

CheckMate™/Flexi® Vector Mammalian Two-Hybrid System

The CheckMate™/Flexi® Vector Mammalian Two-Hybrid System provides a means to confirm, validate and study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays. Developed primarily for mammalian proteins of interest, the system can allow protein expression and post-translational modifications in an environment mimicking the native cell milieu. It is patterned on the yeast two-hybrid system with one protein of interest ("X") fused to a DNA-binding domain and the other protein ("Y") fused to a transcriptional activation domain (Figure 1).

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Product Size Conc. Catalog # *List Price QTY Add to Cart

CheckMate™/Flexi® Vector Mammalian Two-Hybrid System

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CheckMate™/Flexi® Vector Mammalian Two-Hybrid System

 Components

  • pFN10A (ACT) Flexi® Vector

    C933B1 x 5μg
  • pFN11A (BIND) Flexi® Vector

    C934B1 x 5μg
  • pGL4.31[luc2P/GAL4UAS/Hygro] Vector

    C935A1 x 20μg
  • pBIND Vector

    E245A1 x 20μg
  • pACT Vector

    E246A1 x 20μg
  • pBIND-Id Control Vector

    E247A1 x 20μg
  • pACT-MyoD Control Vector

    E248A1 x 20μg
1 each
- C9360 $ 748.00 Add to cart


Available Separately

Product Size Conc. Catalog # *List Price QTY Add to Cart

pFN10A (ACT) Flexi® Vector

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pFN10A (ACT) Flexi® Vector

  Components

  • pFN10A (ACT) Flexi® Vector

    C933A1 x 20μg
20μg
- C9331 $ 298.00 Add to cart

pFN11A (BIND) Flexi® Vector

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pFN11A (BIND) Flexi® Vector

  Components

  • pFN11A (BIND) Flexi® Vector

    C934A1 x 20μg
20μg
- C9341 $ 298.00 Add to cart

pGL4.31[luc2P/GAL4UAS/Hygro] Vector

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pGL4.31[luc2P/GAL4UAS/Hygro] Vector

  Components

  • pGL4.31[luc2P/GAL4UAS/Hygro] Vector

    C935A1 x 20μg
20μg
- C9351 $ 372.00 Add to cart

CheckMate™ Positive Control Vectors

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CheckMate™ Positive Control Vectors

  Components

  • pBIND-Id Control Vector

    E247A1 x 20μg
  • pACT-MyoD Control Vector

    E248A1 x 20μg
1 set
- C9370 $ 204.00 Add to cart

CheckMate™ Negative Control Vectors

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CheckMate™ Negative Control Vectors

  Components

  • pBIND Vector

    E245A1 x 20μg
  • pACT Vector

    E246A1 x 20μg
1 set
- C9380 $ 135.00 Add to cart

GenBank®/EMBL Accession Number

pFN10A(ACT) Flexi® Vector Accession Number DQ487211; pFN10A(ACT) Flexi® Vector sequence text file.
pFN11A(BIND) Flexi® Vector Accession Number DQ487212; pFN11A(BIND) Flexi® Vector sequence text file.
pGL4.31[luc2P/GAL4UAS/Hygro] Accession Number DQ487213; pGL4.31[luc2P/GAL4UAS/Hygro] sequence text file.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

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