HisLink™ Protein Purification Resin

HisLink™ Protein Purification Resin is a macroporous silica resin modified to contain a high level of tetradentate chelated nickel (>20mmol Ni/ml settled resin). The resin is designed to efficiently capture and purify bacterially expressed polyhistidine- or HQ-tagged proteins. The HisLink™ Resin also may be used for general applications requiring an immobilized metal affinity chromatography (IMAC) matrix. HisLink™ Resin may be used in either column or batch purification formats. The lysate flows by gravity over a column of packed HisLink™ Resin at a rate sufficient for complete capture and efficient elution of ...

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HisLink™ Protein Purification Resin is a macroporous silica resin modified to contain a high level of tetradentate chelated nickel (>20mmol Ni/ml settled resin). The resin is designed to efficiently capture and purify bacterially expressed polyhistidine- or HQ-tagged proteins. The HisLink™ Resin also may be used for general applications requiring an immobilized metal affinity chromatography (IMAC) matrix. HisLink™ Resin may be used in either column or batch purification formats. The lysate flows by gravity over a column of packed HisLink™ Resin at a rate sufficient for complete capture and efficient elution of polyhistidine- or HQ-tagged proteins from cleared lysate. The resin may be used in vacuum filtration devices (e.g., Vac-Man® Vacuum Manifold [Cat.# A7231]), allowing simultaneous processing of multiple columns. HisLink™ Resin is also an excellent choice for affinity purification of polyhistidine- or HQ-tagged proteins with low- to medium-pressure liquid chromatography systems such as FPLC. In batch format, HisLink™ Resin may be separated easily from the lysate without filtration (or centrifugation), allowing processing of larger quantities of lysate and the ability to purify protein without clearing the lysate of insoluble cellular debris.

Features - Benefits

• Optimize Yields: High binding capacity (>15mg/ml).
• Save Time: Purify polyhistidine- or HQ-tagged proteins from cleared or crude cell lysates.
• Flexible: Use standard gravity column chromatography or automated applications such as FPLC.

Applications

• Protein purification of polyhistidine- or HQ-tagged, expressed proteins from a crude E. coli cell lysate.

For more information, see the Protocols & Applications Guide.

Notes

Product volumes represent total volume of a 50% resin slurry.

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