Promega Corporation

Polyhistidine His-Tag Protein Purification

FMG-expression-validated-orf-clones

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Efficient. Optimized. Affordable.

We offer high-capacity, affordable metal-affinity reagents for capture of His-tagged proteins. Systems are available for a variety of throughput needs. 

Featured Products

His Spin and Vacuum Purification

A robust, high-capacity, single-step system with optimized vacuum and centrifugation protocols.

His Magnetic Purification

A flexible, paramagnetic particle-based system for automated protein purification on Beckman Coulter or Tecan robotic workstations.

His 96-Well Protein Purification

Capture 1mg of polyhistidine proteins per well in either manual or automated format. No centrifugation!

Protein G Magnetic Antibody Purification

High-yield, robust purification of antibodies from serum, ascites and cell media.

BenchTop, Automated His Purification

Maxwell® 16 provides an easy method for fast, convenient purification of polyhistidine-tagged proteins from bacterial, mammalian or insect cells in about 40 minutes.

Magnetic Stands and Spacers

A wide range of magnetic platforms from 50ml conicals to microcentrifuge tubes to 96- and 384-well plates for all your magnetic biomolecule separation needs.

Featured Resources

pubhub_fr HisLink™ Spin Protein Purification System: Maximum Versatility in a Small Package

In this article we describe the HisLink™ Spin Protein Purification System, which is capable of purifying 1mg of polyhistidine- or HQ-tagged proteins using a single spin column.

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