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The HaloTag® Protein Purification System is designed to purify proteins fused to the HaloTag® novel protein tag that enhances the expression and solubility of recombinant proteins. HaloTag® Technology enables the covalent, efficient and specific capture of a protein of interest onto HaloLink™ Resin, thus overcoming the equilibrium-based limitations associated with affinity tags (i.e., poor capture of proteins expressed at low levels and protein loss during washing of the purification resin.
The HaloTag® technology offers a quick and convenient way to test protein expression of HaloTag® fusio...
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The HaloTag® technology offers a quick and convenient way to test protein expression of HaloTag® fusion proteins as well as monitor the efficiency of immobilization to HaloLink™ Resin by labeling with fluorescent HaloTag® TMR Ligand followed by SDS-PAGE analysis. Guidelines can be found in the HaloLink™ Resin Technical Manual #TM250, the HaloLink™ Protein Array Technical Manual #TM310 and the HaloCHIP™ System Technical Manual #TM075.
Outline of Procedure
The HaloTag® protein, a 34kDa mutated hydrolase, covalently attaches to HaloLink™ Resin via an immobilized chloroalkane ligand. TEV Protease cleaves the target protein from the HaloLink™ Resin. The TEV Protease, which has an N-terminal (HQ) tag, is removed from the protein of interest using HisLink™ Resin, and the purified protein of interest is recovered. The appropriate vector that encodes the HaloTag® protein and expresses protein optimally in E. coli is pFN18A HaloTag® T7 Flexi® Vector (G2751) or pFN18K HaloTag® T7 Flexi® Vector (G2681). These vectors can be purchased separately.
Cat.# G6280 contains 100ml of a 25% slurry of HaloLink™ Resin, TEV Protease and HisLink™ Resin. Cat.# G6270 contains 10ml of a 25% slurry of HaloLink™ Resin, TEV Protease and HisLink™ Resin.
Competitive pricing is available for bulk quantities of HaloLink™ Resin (e.g., more than 100 ml). Please inquire.
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HaloTag® Protein Purification System
TEV Protease for use with HisLink™ Resin
HisLink™ Protein Purification Resin
HaloTag® Protein Purification System Sample Pack
Store the HaloLink™ Resin and HisLink™ Resin at 4°C. Do not freeze the resins. Store the TEV Protease at –20°C.
For product intended use please see Patents & Disclaimers tab.
Covalent, irreversible binding of HaloTag® fusion tag to the HaloLink™ Resin maximizes purification yields when compared to other fusion tags.
G6280, G6270 For Research Use Only. Not for Use in Diagnostic Procedures.
G6280, G6270 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may only use this product in their own research and may modify or link Promega HaloTag® ligands to Promega or customer-supplied moieties. No other use or transfer of this product is authorized without the express, written consent of Promega including, without limitation, commercial use. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
G6280, G6270 U.S. Pat. Nos. 7,112,552 and 7,354,750.
G6280, G6270 U.S. Pat. Nos. 7,429,472, 7,888,086, 8,202,700 and other patents pending.
G6280, G6270 Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.
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