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The HaloLink™ Resin provides a method for covalent and oriented attachment of HaloTag® fusion proteins onto a solid surface. The resin consists of a HaloTag® ligand bound to Sepharose® beads that specifically and rapidly binds HaloTag® fusion proteins. HaloLink™ Resin has high binding capacity. Due to covalent linkage, HaloTag® fusion proteins cannot be eluted from the resin, allowing extensive washing to remove nonspecifically bound protein without the danger of eluting HaloTag® fusion proteins. The binding rate is very rapid and equivalent to biotin-streptavidin.
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The HaloLink™ Resin can be used in a variety of applications including: detection and analysis of protein:protein interactions (in vivo and in vitro), detection of enzymatic activity of immobilized HaloTag® fusions and one-step purification of fusion protein in conjunction with proteolytic cleavage. A variety of vectors for the expression of HaloTag® fusion proteins in bacterial, mammalian or cell-free systems are available. Please see the HaloTag® Technology Products page for more information.
The HaloTag® technology offers a quick and convenient way to test protein expression of HaloTag® fusion proteins as well as monitor the efficiency of immobilization to HaloLink™ Resin by labeling with fluorescent HaloTag® TMR Ligand followed by SDS-PAGE analysis. Guidelines can be found in the HaloLink™ Resin Technical Manual #TM250.
Note: Size listed indicates amount of settled resin.
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This product is available through the Promega Helix onsite stocking program in a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs. The Helix Refrigerator is 5 cubic ft.
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Panels A and B illustrate two approaches to characterizing protein:protein interactions. Panel C shows an analysis of protein:protein interaction.
G1912, G1913, G1914, G1915 For Research Use Only. Not for Use in Diagnostic Procedures.
G1912, G1913, G1914, G1915 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
G1912, G1913, G1914, G1915 U.S. Pat. Nos. 7,429,472, 7,888,086, 8,202,700 and other patents pending.
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