Choose the system that best meets your recombinant protein capture needs.
HaloTag® Purification from Mammalian Cells
This system offers strong improvements in recovery, purity, cost and protein functionality over FLAG® and polyhistidine tag purification strategies.
HaloTag® Purification from E. coli
A highly efficient system for soluble, tag-free purified protein—even when expressed at low levels.
HaloTag® Pull-Down for Protein:Protein Interactions
Capture novel in vivo protein interactions and complexes with ultra-low background.
HaloTag® Pull-Down for Protein:DNA Interactions
No antibody required! Identify protein:DNA interactions in 24-48 hours with a simple, proven ChIP system.
His Spin and Vacuum Purification
A robust, high-capacity, single-step system with optimized vacuum and centrifugation protocols.
Biotinylated Column and Batch Purification
A complete system for purifying in vivo biotinylated proteins under gentle, non-denaturing conditions.
A protocol that provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels.
This article describes how purification using HaloTag® technology gives high protein yield and purity, with no protein tag carryover.