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Promega Corporation

ReliaPrep™ FFPE Miniprep Systems

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ReliaPrep™ FFPE Miniprep Systems provide complete, all-inclusive methods for purification of gDNA and total RNA from formalin-fixed paraffin-embedded tissues.

  • Isolate quality, intact nucleic acids: Optimized lysis and binding conditions reverse modifications introduced by the fixation process, resulting in intact, amplifiable nucleic acids.
  • Safely deparaffinize your sample: Deparaffinization step occurs without harsh organic solvents.
  • Save time: Purify gDNA and total RNA from FFPE tissue in less than two and one half hours with minimal hands on time. No overnight digestion required.
  • Easy to use: Minimal preparation time; simply add ethanol and go!
Figure 10139CA

Samples were analyzed using GoTaq® qPCR System. Earlier Cq values were obtained with the ReliaPrep™ FFPE Miniprep System.

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Genomic DNA purified from a single 10µm mouse brain section using either the ReliaPrep™ FFPE gDNA Miniprep System or competitor kit.
Figure 10140TA

More large-fragment RNA was purified with the ReliaPrep™ FFPE Total RNA Miniprep System than with competitor kits.

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Total RNA purified from sequential 10µm mouse liver FFPE sections analyzed on an Agilent Bioanalyzer.
Figure 10142TB

Panel A. 10μl purified gDNA used as template in multiplex PCR (GoTaq® Hot Start). Fragments ranging from 100-400bp detected.
Panel B. 5μl purified RNA used as template in GoScript™ Reverse Transcription System. 5μl of cDNA product used as template for the multiplex PCR (GoTaq® Hot Start). Fragments from 80bp-290bp detected.
All reactions performed in triplicate from sequential slices. 10μl PCR product run on a 2% agarose gel.

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Multiplex PCR of variable length, end-point PCR fragments of GAPDH gene from nucleic acid purified from 10μm slices of FFPE human liver.
Figure 10187TA

STR analysis was performed using Promega's Microsatellite Instability (MSI) Analysis System v. 1.2 (Cat.# MD1641). This system is compatible with STR amplification and MSI detection.

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Genomic DNA was purified from 10µm human liver FFPE sections using the ReliaPrep™ FFPE gDNA Miniprep System.
Figure 10397TA

Sample Type: Human cervical cancer, 3 years old
Description: Genomic DNA was extracted using Qiagen (lane 1) and Promega ReliaPrep FFPE kits (lanes 2-5). Lane M = DNA marker, Lane N = blank.

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ReliaPrep™ FFPE gDNA Miniprep – Customer Data
Figure 10398TA

Sample Type: Rat kidney, 2 years old
Description: Four 10µm FFPE sections were purified together (total amount = 40µm) and diluted in 40µl. Eight microliters were used for gel electrophoresis.

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ReliaPrep™ FFPE Total RNA Miniprep – Customer Data

Sample Type: Human liver tumor, fixed less than 1 year
Description: One microliter out of 40µl total dilution of RNA was used for the gel.

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ReliaPrep™ FFPE Total RNA Miniprep – Customer Data
Jinpeng He

Sample Type: Human kidney tumor, 2 months old
Description: Two samples were extracted using the total RNA kit. The total dilution volume was 30µl, and the loading volume for agarose gel electrophoresis was 15µl.
Purity: A260 / A280 ~2.0
A260 / A230 ~2.1
Concentration: 200ng/µl

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ReliaPrep™ FFPE Total RNA Miniprep – Customer Data

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