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Promega Corporation

Experience the Power of a Single Tag

The HaloTag® technology is a comprehensive platform for functional protein analysis. It is based on the formation of a covalent bond between a protein fusion tag (HaloTag®) and synthetic chemical ligands. By interchanging different synthetic ligands researchers ultimately control the function and properties of the HaloTag® fusion protein. The HaloTag® system is designed to provide broad experimental flexibility and superb performance in both cell-based and biochemical assays without switching fusion tags, or recloning. Available HaloTag® ligands include magnetic and non-magnetic beads, glass slides, cell-permeable and impermeable fluorescent ligands, biotin, and reactive ligands to enable researchers to build their own custom HaloTag® ligands and surfaces. The diversity of HaloTag® ligands allows a large number of different applications including, protein purification, protein interaction discovery, protein arrays, cell-based assays for protein translocation, receptor internalization, protein stability.

HaloTag® Solutions

2013 Breakthrough Discoveries in Epigenetics Using HaloTag® Technology

"KDM4A Lysine Demethylase Induces Site-Specific Copy Gain and Rereplication of Regions Amplified in Tumors"

Black, J. et al. (2013) Cell, 154 (3), 541-55.

Click to read article »

"HIF1A Employs CDK8-Mediator to Stimulate RNAPII Elongation in Response to Hypoxia"

Galbraith et al. (2013) Cell, 153 (6), 1327-39.

Click to read article »

"TET2 and TET3 regulate GlcNAcylation and H3K4 methylation through OGT and SET1/COMPASS"

Deplus, R. et al. (2013). EMBO J. 32, 645–50.

Click to read article »

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Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.