STR Systems for Cell Line Authentication
Protect Your Scientific Conclusions
- Misidentification or cross-contamination of cells and cell lines is a common problem
- 15-20% of ALL cells are misidentified or contaminated
- The implications of misidentification are far-reaching
- Invalidation of published data
- Wasted time, effort and money
- STR analysis plays a critical role in cell authentication
- Recommended in ANSI/ATCC guidelines
- Recommended by NIH and many journals
STR Cell Line Authentication Workflow
Options for Authenticating Your Cell Lines
- Set up the STR analysis workflow in your lab.
- Establish an STR core facility at your institution to process samples for many researchers.
- Use an already established service provider such as ATCC to process and analyze your samples.
Promega offers a choice of STR analysis kits for cell line authentication. The PowerPlex® 18D System offers direct amplification and four-color fluorescent detection of seventeen STR loci and Amelogenin for highest power of discrimination. The StemElite™ ID and Cell ID™ Systems amplify 9 STR loci plus amelogenin; the StemElite™ ID System also enables identification of contaminating mouse feeder cells in a cultured cell population.
Example Data
An example StemElite™ ID multiplex amplification reaction performed with 2ng of H9 hESC DNA. Amplification products were analyzed by capillary electrophoresis and products for each fluorescent channel graphed as base pair size versus fluorescence. Sizes were determined for each sample peak using the Internal Lane Standard 600 (Cat.# DG2611). An allelic ladder was analyzed along with the unknown samples and, like the samples, a size assigned for each peak.
Cell line authentication services are offered by these providers: