Promega's Caspase-Glo® Assays produce a linear response with caspase concentration and have signal windows that are several orders of magnitude greater than fluorescent-based substrate assays that require extended incubations to achieve significant signal and suffer from high intrinsic background fluorescence that limits signal to noise ratios.
Use less enzyme and reduce assay costs.
The Caspase-Glo 3/7 Assay provides superior sensitivity and linearity over 4 orders of magnitude of caspase concentration.
Caspase-Glo 8 and 9
>Caspase-Glo 8 and 9 Assays are more sensitive than comparable fluorescent assays, generating large signal windows with broad and linear dynamic ranges.
Caspase-Glo 2 and 6
>Human recombinant caspase-2 and -6 (BIOMOL) were titrated in 10 mM HEPES (pH 7.0), 2 mM DTT and assayed in 96-well plates.
>Panel A. The Caspase-Glo® 2 Assay (Z-VDVAD-Glo™ Substrate) or comparable fluorogenic substrates, Z-VDVAD-AFC and Ac-VDVAD-AMC, were tested with caspase-2. Luminescence and fluorescence were measured at 30 and 60 minutes on a GloMax® 96 Microplate Luminometer or a Labsystems Fluoroskan Ascent plate reader, respectively. AFC fluorescence was also measured at 18 hours.
>Panel B. The Caspase-Glo® 6 Assay (Z-VEID-Glo™ Substrate) or an Ac-VEID-AFC fluorescent substrate were tested with caspase-6. Luminescence and fluorescence were monitored after 60 minutes on a BMG FLUOstar multimode reader. For both graphs, the results are plotted as signal-to-noise ratios. The limit of detection is defined as the amount of caspase giving a signal-to-noise ratio >3 (dashed lines). The signal-to-noise ratio is greater, and the limit of detection is significantly lower for the luminescence assays compared to the fluorescence assays.
User fewer cells and reduce cell culture costs.
>The Caspase-Glo 3/7 Assay provides superior sensitivity and linearity over a broad range of cell numbers.
Caspase-Glo 8 and 9
The Caspase-Glo 8 Assay (A) or Caspase-Glo 9 (B) Assay provides superior sensitivity and linearity over a broad range of cell numbers.
Use less compound and save on synthesis costs.