ADP-Glo Kinase Assay Work Flow
The New Gold Standard in Kinase Assays
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Sensitive down to 20nM ADP: Less enzyme needed per reaction thus reducing costs; easily miniaturizable.
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Strong signal at low % conversion of substrate: Obtain meaningful results under initial reaction conditions; also means less enzyme is required.
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Simple to use: No time wasted optimizing or learning to perform assays; antibody-free.
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Works with broad range of kinases: Assay more kinases in-house— reduces dependency on outsourcing.
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Accurate IC50s— results comparable to radio-labeled assays: Allows researchers to switch to a safer method with the peace of mind that their results are comparable to the "gold standard".
Sensitivity and linearity of ADP-Glo Kinase Assay
Each graph represents the total concentration of nucleotide (ATP + ADP) in the reaction.
Strong signal to background at low % conversion of substrate
| Enzyme |
SB5* |
% ATP to ADP** conversion at SB5 |
| EGFR |
0.3ng |
2.2 |
| PDGFRα |
21ng |
4.7 |
| VEGFR2 (KDR) |
1ng |
4.2 |
| DNA-PK |
0.4ng |
2.7 |
| AKT2 |
7ng |
4.3 |
| IKKβ |
4.5ng |
2.9 |
| MAPK (ERK2) |
1ng |
5.6 |
| PKA |
0.013 units |
0.35 |
| Hexoklnase |
0.1 units |
4.6 |
| PI3 Kinase γ |
0.15ng |
2 |
| Sphingosine Kinase 1 |
0.8ng |
2.9 |
| MDR1 (P-gp) |
25μg (SB20) |
50 |
| Na+/K+ ATPase |
1ng |
3 |
* Amount of enzyme required to produce a signal to background ratio of 5 using ADP-Glo.
** Enzyme reactions were performed at different ATP Concentrations.
Works with broad range of kinases
Accurate IC50 Values
