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Promega Corporation

Pfu DNA PolymerasePCR Satisfaction Guaranteed

Pfu DNA Polymerase is a thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. Consequently, Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated ...

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Source

Pyrococcus furiosus strain Vc1 DSM3638.

Storage Buffer

50mM Tris-HCl (pH 8.2 at 25°C), 0.1mM EDTA, 1mM DTT, 50% glycerol and 0.05% CHAPS.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTPs into acid-insoluble material in 30 minutes at 75°C.

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