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Improve your protein sample prep workflow with fast, convenient digestion, improved sequence coverage and enhanced data from minimal sample material.
Use of ProteaseMAX™ for Cell Lysis Prior to Mass Spectrometry
View all Proteases for Mass Spec
Unique Applications of Alternative Proteases
Trypsin/Lys-C Mix, Mass Spec Grade
Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The mix is designed to improve digestion of proteins or protein mixtures in solution.
Recommended for use in standard in-solution protein digestion and in-solution digestion of proteolytically resistant proteins.
ProteaseMAX™ Surfactant, Trypsin Enhancer
Fast, high-quality protein digestion when used in combination with trypsin. Streamlined in-gel digestion protocol.
Trypsin Gold, Mass Spectrometry Grade
Manufactured for maximum specificity. Lysine residues in porcine trypsin have been modified by reductive methylation, yielding a highly active, stable molecule that is extremely resistant to autolytic digestion. Specificity is further improved by TPCK treatment. Each lot is qualified for in-gel digestion and mass spectrometric analysis.
rLys-C, Mass Spec Grade
A recombinant Lys-C expressed in E. coli and similar to native Lys-C, rLys-C cleaves at the carboxyl side of lysine residues with exceptional specificity. rLys-C retains proteolytic activity under protein denaturing conditions such as 8M urea, which is used to improve digestion of proteolytically resistant proteins. rLys-C activity is optimal in the pH range of 8–9.
An article describing the cleavage specificity of endoproteinases Asp-N and Glu-C, and showing an example of improved protein sequence coverage through the use of alternative digestion with these enzymes.
We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.
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