Proteases and Surfactants

Trypsin/Lys-C Mix, Mass Spec Grade

Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The mix is designed to improve digestion of proteins or protein mixtures in solution.

Recommended for use in standard in-solution protein digestion and in-solution digestion of proteolytically resistant proteins.

ProteaseMAX™ Surfactant, Trypsin Enhancer

Fast, high-quality protein digestion when used in combination with trypsin. Streamlined in-gel digestion protocol.

Trypsin Gold, Mass Spectrometry Grade

Manufactured for maximum specificity. Lysine residues in porcine trypsin have been modified by reductive methylation, yielding a highly active, stable molecule that is extremely resistant to autolytic digestion. Specificity is further improved by TPCK treatment. Each lot is qualified for in-gel digestion and mass spectrometric analysis.

rLys-C, Mass Spec Grade

A recombinant Lys-C expressed in E. coli and similar to native Lys-C, rLys-C cleaves at the carboxyl side of lysine residues with exceptional specificity. rLys-C retains proteolytic activity under protein denaturing conditions such as 8M urea, which is used to improve digestion of proteolytically resistant proteins. rLys-C activity is optimal in the pH range of 8–9.

Alternative Proteases

Digestion with alternative proteases alone or with trypsin can result in a unique peptide map that may include sequences not seen with trypsin digestion alone.

Trypsin for Mass Spectrometry

We provide a variety of trypsins in which lysine residues are modified by reductive methylation, resulting in a highly active, stable molecule that is extremely resistant to autolytic digestion.

Featured Resources

Using Endoproteinases Asp-N and Glu-C to Improve Protein Characterization