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Promega Corporation

Proteases and Surfactants

accessWebinarMS Compatible Protein Extracts Reference Material for Instrument Monitoring

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Enhance Your Sample Prep Research

Improve your protein sample prep workflow with fast, convenient digestion, improved sequence coverage and enhanced data from minimal sample material.

Use of ProteaseMAX™ for Cell Lysis Prior to Mass Spectrometry

View all Proteases for Mass Spec

Unique Applications of Alternative Proteases

Featured Products

Trypsin/Lys-C Mix, Mass Spec Grade

Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The mix is designed to improve digestion of proteins or protein mixtures in solution.

Recommended for use in standard in-solution protein digestion and in-solution digestion of proteolytically resistant proteins.

ProteaseMAX™ Surfactant, Trypsin Enhancer

Fast, high-quality protein digestion when used in combination with trypsin. Streamlined in-gel digestion protocol.

Trypsin Gold, Mass Spectrometry Grade

Manufactured for maximum specificity. Lysine residues in porcine trypsin have been modified by reductive methylation, yielding a highly active, stable molecule that is extremely resistant to autolytic digestion. Specificity is further improved by TPCK treatment. Each lot is qualified for in-gel digestion and mass spectrometric analysis.

rLys-C, Mass Spec Grade

A recombinant Lys-C expressed in E. coli and similar to native Lys-C, rLys-C cleaves at the carboxyl side of lysine residues with exceptional specificity. rLys-C retains proteolytic activity under protein denaturing conditions such as 8M urea, which is used to improve digestion of proteolytically resistant proteins. rLys-C activity is optimal in the pH range of 8–9.

Featured Resources

pubhub_fr Using Endoproteinases Asp-N and Glu-C to Improve Protein Characterization

An article describing the cleavage specificity of endoproteinases Asp-N and Glu-C, and showing an example of improved protein sequence coverage through the use of alternative digestion with these enzymes.

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